白花蛇舌草的化学成分研究

Chemical Constituents from Hedyotis diffusa Willd.

摘要:

为研究白花蛇舌草（Hedyotis diffusa）的化学成分，采用硅胶柱色谱、制备HPLC色谱等方法，从白花蛇舌草全草的70%乙醇提取物中分离得到9个化合物。根据理化性质及其波谱或光谱数据，结构分别鉴定为：豆甾醇（1）、β-谷甾醇（2）、rubiadin （3）、2，6-二羟基-1-甲氧基蒽醌（4）、β-谷甾醇-3-O-β-d-葡萄糖苷（5）、对香豆酸（6）、山奈酚（7）、槲皮素（8）和2-羟基-3-甲氧基-7-甲基蒽醌（9）。化合物4为新化合物；氯仿提取部位的Fr.4、Fr.4-2及Fr.4-4流分具有较强体外抗人子宫内膜癌Ishikawa细胞活性，其IC₅₀值分别为52.8、78.1和27.5 μg/mL。

Abstract:

To understand the chemical composition of Hedyotis diffusa, nine compounds were isolated from the 70% ethanol extract by column chromatography on silica gel and preparative high-performance liquid chromatography. Based on physicochemical properties and spectral data, their structures were identified as stigmasterol (1), β-sitosterol (2), rubiadin (3), 2,6-dihydroxy-1-methoxyanthraquinone (4), β-sitosterol-3-O-β-d-glucoside (5), p-coumaric acid (6), kaempferol (7), quercetin (8) and 2-hydroxy-3-methoxy-7-methylanthraquinone (9). Compound 4 was a new compound. The Fr.4, Fr.4-2 and Fr.4-4 fractions in chloroform extract had strong activity against human endometrial carcinoma Ishikawa cells in vitro, with IC₅₀ of 52.8, 78.1 and 27.5 μg/mL, respectively.