铁皮石斛和重唇石斛及其杂交子代花的挥发性成分分析

Volatile Components in Flowers of Dendrobium officinale, D. hercoglossum and Their Hybrids

摘要:

为阐明铁皮石斛和重唇石斛及其杂交后代14L-3、14L-6、14L-7和14L-9花挥发性成分的变化，采用静态顶空气相色谱-质谱(GC-MS)联用技术对石斛花进行检测。结果表明，从石斛花中检测出81种挥发性成分，包括烯、酮、醛、烷几大类,铁皮石斛、重唇石斛、14L-3、14L-6、14L-7和14L-9分别有23、12、21、33、23和35种。铁皮石斛花的主成分是α-蒎烯,重唇石斛花是2-十五烷酮，4个子代花中均含有这2种来自亲本的特征成分，α-蒎烯是子代共同的主成分。亲本和子代石斛花共有成分是正己醛。子代14L-3、14L-6、14L-9均与母本铁皮石斛相似性高，相似性以14L-3>14L-6>14L-9，与父本差异性大。14L-7与亲本相似度最均衡。这为石斛育种研究提供了指导。

Abstract:

To clarify the changes in volatile components of Dendrobium officinale, D. hercoglossum and their hybrid strains (14L-3, 14L-6, 14L-7 and 14L-9), their flowers were detected by static headspace combined with gas chromatography-mass spectrometry (GC-MS) technology. The results showed that there were 81 volatile components detected, including olefins, ketones, aldehydes, and alkanes. Among them, there are 23 compounds in D. officinale, 12 in D. hercoglossum, 21 in 14L-3, 33 in14L-6, 23 in 14L-7, and 35 in 14L-9. The main component in D. officinale flower was α-pinene, and that in D. hercoglossum was 2-pentadecanone. α-Pinene was the main component common in the four hybrids. The parents and hybrids contain hexanal. The hybrids 14L-3, 14L-6, and 14L-9 had high similarity with the female parent D. officinale in the order of 14L-3>14L-6>14L-9, they are quite different from the male parent D. hercoglossum. 14L-7 has the most balanced similarity with the parents. Therefore, these would provide guidance for breeding of Dendrobium.