金边红苞凤梨叶片的超微观察和AbGLK1的克隆与表达分析
作者:
基金项目:

国家自然科学基金项目(31971704, 31770743)资助


Ultrastructure of Leaf and Cloning, Expression of AbGLK1 in Ananas comosus var. bracteatus 'Chiyan'
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [28]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为了解Golden2-like (GLK)转录因子在金边红苞凤梨(Ananas comosus var. bracteatus ‘Chiyan’)绿白嵌合叶片形成中的作用,采用RT-PCR技术克隆得到了AbGLK1基因, 其开放阅读框全长1 371 bp,编码456个氨基酸,含有1个GARP-DNA结合域和1个C末端结构域GCT box (GOLDEN2 C-terminal box),属于GLK转录因子家族,在酵母中具有转录因子的转录激活活性。烟草亚细胞定位表明AbGLK1蛋白定位在细胞核。RT-qPCR分析表明,AbGLK1基因在金边红苞凤梨的根、茎、叶中均有表达,但具有组织器官差异性,在叶片中的表达量显著高于根和茎(P < 0.05)。AbGLK1基因在叶片边缘白化组织中的表达量显著低于绿色组织,约为绿色组织的1/3 (P < 0.05)。叶片白化组织叶绿体内膜系统模糊,无类囊体存在,含有大量囊状小泡,质体小球数量多且体积较大。因此,推测AbGLK1基因可能参与了金边红苞凤梨中的叶绿体发育,其下调表达可能导致叶片白化组织中叶绿体发育不成熟。

    Abstract:

    In order to understand the function of Golden2-like (GLK) in green-white chimeric leaf formation of Ananas comosus var. bracteatus 'Chiyan', AbGLK1 gene was cloned by RT-PCR. The results showed that AbGLK1 contained an open reading frame with 1 371 bp, encoding a protein with 456 amino acids. AbGLK1 protein had a GOLDEN2 C-terminal box and a GAPR DNA-binding domain at the N terminal, belonged to GLK transcription factor family, which located in the nucleus. Transactivation analysis showed that AbGLK1 had transcriptional activation activity. And AbGLK1 was tissue-specific expressed in root, stem and leaf of 'Chiyan'. The expression of AbGLK1 in leaves was significantly higher than that in roots and stems (P < 0.05); which in albino tissues was about 1/3 in green tissues (P < 0.05). In albino tissue, there was no thylakoid in chloroplast, but large number of plastoglobulus and vesicles exist in chloroplast. Therefore, it is suggested that AbGLK1 might be involved in chloroplast development, and down expression of AbGLK1 might lead to immature chloroplast in albino tissue.

    参考文献
    [1] MA J, KANAKALA S, HE Y H, et al. Transcriptome sequence analysis of an ornamental plant, Ananas comosus var. bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosyn- thesis [J]. PLoS One, 2015, 10(3): e0119153. doi: 10.1371/journal. pone.0119153.
    [2] ZHU M K, HU Z L, ZHOU S, et al. Research progress of plant leaf albino[J]. Chin Bull Life Sci, 2012, 24(3): 255-261. doi: 10.13376/j. cbls/2012.03.011. 朱明库, 胡宗利, 周爽, 等. 植物叶色白化研究进展[J]. 生命科学, 2012, 24(3): 255-261. doi: 10.13376/j.cbls/2012.03.011.
    [3] XUE Y B, MA J, HE Y H, et al. Comparative transcriptomic and proteomic analyses of the green and white parts of chimeric leaves in Ananas comosus var. bracteatus [J]. PeerJ, 2019, 7: e7261. doi: 10. 7717/peerj.7261.
    [4] MAO M Q, XUE Y B, HE Y H, et al. Systematic identification and comparative analysis of lysine succinylation between the green and white parts of chimeric leaves of Ananas comosus var. bracteatus [J]. BMC Genom, 2020, 21(1): 383. doi: 10.1186/s12864-020-67 50-6.
    [5] DU W K, YUAN S X, HU F R. Research progress on molecular mechanisms of the leaf color mutation[J]. Mol Plant Breed, 2019, 17(6): 1888-1897. doi: 10.13271/j.mpb.017.001888. 杜文凯, 袁素霞, 胡凤荣. 植物叶色突变分子机制的研究进展[J]. 分子植物育种, 2019, 17(6): 1888-1897. doi: 10.13271/j.mpb.017.001 888.
    [6] YANG H Y. Study on mechanism of leaf color variation of Pseudosasa japonica f. akebonosuji[D]. Beijing: Beijing Forestry University, 2015: 39-56. 杨海芸. 花叶矢竹叶色变异机理研究[D]. 北京: 北京林业大学, 2015: 39-56.
    [7] LI N N. Physiological, biochemical characteristics and molecular albinism of the albino tea (Camellia sinensis) plant[D]. Hangzhou: Zhejiang University, 2015: 14-24. 李娜娜. 新梢白化茶树生理生化特征及白化分子机理研究[D]. 杭州: 浙江大学, 2015: 14-24.
    [8] FITTER D W, MARTIN D J, COPLEY M J, et al. GLK gene pairs regulate chloroplast development in diverse plant species[J]. Plant J, 2002, 31(6): 713-727. doi: 10.1046/j.1365-313X.2002.01390.x.
    [9] HALL L N, ROSSINI L, CRIBB L, et al. GOLDEN 2: A novel trans- criptional regulator of cellular differentiation in the maize leaf[J]. Plant Cell, 1998, 10(6): 925-936. doi: 10.1105/tpc.10.6.925.
    [10] ROSSINI L, CRIBB L, MARTIN D J, et al. The maize Golden2 gene defines a novel class of transcriptional regulators in plants[J]. Plant Cell, 2001, 13(5): 1231-1244. doi: 10.2307/3871376.
    [11] YASUMURA Y, MOYLAN E C, LANGDALE J A. A conserved transcription factor mediates nuclear control of organelle biogenesis in anciently diverged land plants[J]. Plant Cell, 2005, 17(7): 1894-1907. doi: 10.1105/tpc.105.033191.
    [12] POWELL A L T, NGUYEN C V, HILL T, et al. Uniform ripening encodes a Golden 2-like transcription factor regulating tomato fruit chloroplast development[J]. Science, 2012, 336(6089): 1711-1715. doi: 10.1126/science.1222218.
    [13] LI G W, CHEN D Y, TANG X F, et al. Heterologous expression of kiwifruit (Actinidia chinensis) GOLDEN2-LIKE homolog elevates chloroplast level and nutritional quality in tomato (Solanum lycoper- sicum)[J]. Planta, 2018, 247(6): 1351-1362. doi: 10.1007/s00425-018 2853-6.
    [14] LUPI A C D, LIRA B S, GRAMEGNA G, et al. Solanum lycopersicum GOLDEN 2-LIKE 2 transcription factor affects fruit quality in a light- and auxin-dependent manner[J]. PLoS One, 2019, 14(2): e0212224. doi: 10.1371/journal.pone.0212224.
    [15] LI C L, NI D J. Effects of aluminium on photosynthesis and ultra- structure in leaves of tea plant[J]. Hubei Agric Sci, 2014, 53(3): 604- 606. doi: 10.3969/j.issn.0439-8114.2014.03.028. 李春雷, 倪德江. 铝对茶树光合特性和叶片超微结构的影响[J]. 湖北农业科学, 2014, 53(3): 604-606. doi: 10.3969/j.issn.0439-8114. 2014.03.028.
    [16] YANG Z R, WANG X C, LI X M, et al. Advance on the study of transcription factors in higher plants[J]. Hereditas, 2004, 26(3): 403- 408. doi: 10.3321/j.issn:0253-9772.2004.03.027. 杨致荣, 王兴春, 李西明, 等. 高等植物转录因子的研究进展[J]. 遗传, 2004, 26(3): 403-408. doi: 10.3321/j.issn:0253-9772.2004.03. 027.
    [17] LI Y H, WANG B H, DAI Z Y, et al. Morphological structure and genetic mapping of a new leaf color mutant in rice[J]. Chin J Rice Sci, 2011, 25(6): 587-593. doi: 10.3969/j.issn.1001-7216.2011.06.004. 李育红, 王宝和, 戴正元, 等. 一个水稻新型叶色突变体的形态结构与遗传定位[J]. 中国水稻科学, 2011, 25(6): 587-593. doi: 10. 3969/j.issn.1001-7216.2011.06.004.
    [18] YANG L, GUO A G, GUAN X. Studies on the chloroplast ultra- structure of "stage albinism line the winter wheat" (SA) mutant during the albescent period[J]. Acta Agric Boreali-Occid Sin, 2003, 12(4): 64-67. doi: 10.3969/j.issn.1004-1389.2003.04.018. 杨莉, 郭蔼光, 关旭. 小麦突变体返白系返白阶段叶绿体超微结构变化研究[J]. 西北农业学报, 2003, 12(4): 64-67. doi: 10.3969/j.issn. 1004-1389.2003.04.018.
    [19] JANIK E, SZCZEPANIUK J, MAKSYMIEC W. Organization and functionality of chlorophyll-protein complexes in thylakoid membranes isolated from pb-treated secale cereale[J]. J Photobiol B Biol, 2013, 125: 98-104. doi: 10.1016/j.jphotobiol.2013.05.008.
    [20] WANG P, FOURACRE J, KELLY S, et al. Evolution of GOLDEN2- LIKE gene function in C3 and C4 plants[J]. Planta, 2013, 237(2): 481- 495. doi: 10.1007/s00425-012-1754-3.
    [21] WATERS M T, WANG P, KORKARIC M, et al. GLK transcription factors coordinate expression of the photosynthetic apparatus in Arabi- dopsis [J]. Plant Cell, 2009, 21(4): 1109-1128. doi: 10.1105/tpc.108. 065250.
    [22] TATUSOV R L, KOONIN E V, LIPMAN D J. A genomic perspective on protein families[J]. Science, 1997, 278(5338): 631-637. doi: 10. 1126/science.278.5338.631.
    [23] NAKAMURA H, MURAMATSU M, HAKATA M, et al. Ectopic overexpression of the transcription factor OsGLK1 induces chloroplast development in non-green rice cells [J]. Plant Cell Physiol, 2009, 50 (11): 1933-1949. doi: 10.1093/pcp/pcp138.
    [24] HAO X Y, ZHANG W F, LIU Y, et al. Pale green mutant analyses reveal the importance of CsGLKs in chloroplast developmental regu- lation and their effects on flavonoid biosynthesis in tea plant[J]. Plant Physiol Biochem, 2020, 146: 392-402. doi: 10.1016/j.plaphy.2019. 11.036.
    [25] LI W X, YANG S B, LU Z G, et al. Cytological, physiological, and transcriptomic analyses of golden leaf coloration in Ginkgo biloba L. [J]. Hort Res, 2018, 5(1): 12. doi: 10.1038/s41438-018-0015-4.
    [26] YANG Y X, CHEN X X, XU B, et al. Phenotype and transcriptome analysis reveals chloroplast development and pigment biosynthesis together influenced the leaf color formation in mutants of Anthurium andraeanum 'Sonate'[J]. Front Plant Sci, 2015, 6: 139. doi: 10.3389/ fpls.2015.00139.
    [27] LI Y, ZHANG Z Y, WANG P, et al. Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant[J]. Genes Genom, 2015, 37 (10): 851-863. doi: 10.1007/s13258-015-0317-y.
    [28] GANG H X, LI R H, ZHAO Y M, et al. Loss of GLK1 transcription factor function reveals new insights in chlorophyll biosynthesis and chloroplast development [J]. J Exp Bot, 2019, 70(12): 3125-3138. doi: 10.1093/jxb/erz128.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

毛美琴,薛彦斌,胡豪,周徐子鑫,马均.金边红苞凤梨叶片的超微观察和AbGLK1的克隆与表达分析[J].热带亚热带植物学报,2022,30(3):311~320

复制
分享
文章指标
  • 点击次数:206
  • 下载次数: 563
  • HTML阅读次数: 593
  • 引用次数: 0
历史
  • 收稿日期:2021-07-09
  • 在线发布日期: 2022-06-07
  • 出版日期: 2022-05-31
文章二维码