丹参SmVS基因的克隆和表达分析

Cloning and Expression Analysis of SmVS Gene from Salvia miltiorrhiza

摘要:

为了解丹参(Salvia miltiorrhiza)的Vinorine合成酶基因(VS)的功能，从丹参中克隆了SmVS基因，并通过Ex PASy等在线分析软件对其进行生物信息学分析，同时利用RT-qPCR技术分析其表达模式。结果表明，SmVS基因全长726 bp，编码241个氨基酸，编码蛋白分子量为26 861.72，等电点PI为5.66，为膜外蛋白，推测定位于线粒体。系统进化分析表明，丹参SmVS与野生油橄榄(Olea europaea var. sylvestris)的VS亲缘关系较近。SmVS基因在丹参根中的表达比叶的高，叶中的转录水平在21:00最高。因此，推测SmVS基因与光应答有关。

Abstract:

In order to understand the function of vinorine synthase gene (VS) in Salvia miltiorrhiza, the SmVS gene were cloned from S. miltiorrhiza, and the bioinformation of SmVS was predicted by Ex PASy and other online analysis software, the expression pattern of SmVS was analyzed by RT-qPCR. The results showed that the full-length of SmVS was 726 bp, encoding 241 amino acids. The molecular weight of SmVS was 26 861.72 with isoelectric point of 5.66. It was an extramembrane protein, presumably located in mitochondria. Phylogenetic tree showed that SmVS had close relationship with VS in Olea europaea var. sylvestris. The expression of SmVS in roots was higher than that in leaves, and the transcription level of SmVS reached the highest at 21:00 pm in leaves. Therefore, it was suggested that SmVS could be involve in responsing to light.