橄榄ISSR和RAPD遗传多样性分析和核心种质构建

ISSR and RAPD Genetic Diversity Analysis and Core Germplasms Construction of Canarium album

摘要:

为揭示中国橄榄（Canarium album）种质资源的遗传多样性，采用ISSR和RAPD标记对橄榄主要分布区的86份种质资源进行遗传多样性分析并构建核心种质。结果表明，基于UPGMA遗传相似系数，86份种质资源可分为3个大类；基于STRUCTURE模型聚类，可分为4个类群，这基本符合橄榄的地域性分布规律。采用ISSR和RAPD获得的中国橄榄种质资源的整体遗传多样性水平分别为0.284±0.169和0.244±0.163，多态性位点百分率分别为92.56%和100%，总遗传分化系数分别为0.127和0.142，基因流分别为3.423和3.025，群体间遗传相似系数分别为0.930和0.939，个体间遗传相似系数分别为0.736和0.732。因此，中国橄榄种质资源丰富的遗传多样性主要来源于个体间的遗传分化或变异，且这种遗传多样性存在明显的地域性差异。

关键词:橄榄;遗传多样性;核心种质;ISSR;RAPD

Abstract:

To reveal the genetic diversity of germplasm resources of Canarium album in China, the genetic diversity of 86 germplasms selected from the major distribution areas was studied by using ISSR and RAPD markers, and core germplasms were constructed. The results showed that 86 germplasms could be divided into 3 groups based on UPGMA genetic similarity, and 4 groups by using STRUCTURE cluster analysis, which basically conform to the regional distribution of C. album in China. The overall genetic diversity coefficient using ISSR and RAPD was 0.284±0.169 and 0.244±0.163, the percentage of polymorphism loci was 92.56% and 100%, the genetic differentiation coefficient was 0.127 and 0.142, the gene flow was 3.423 and 3.025, the average genetic similarity coefficient between populations was 0.930 and 0.939, and the average genetic similarity coefficient between individuals was 0.736 and 0.732. Therefore, it was suggested that the rich genetic diversity of C. album in China mainly caused by the genetic differentiation or variation among individuals, and there were obvious regional differences.

Key words:Canarium album;Genetic diversity;Core germplasm;ISSR;RAPD