首页 > 过刊浏览>2022年第30卷第1期 >41-53. DOI:10.11926/jtsb.4437
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橄榄ISSR和RAPD遗传多样性分析和核心种质构建
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福建省自然科学基金项目（2019J01110）；农业农村部物种品种资源保护（热带作物）项目（151821301354052701）；福建省农业种质资源创新专项（[2021]53）资助


ISSR and RAPD Genetic Diversity Analysis and Core Germplasms Construction of Canarium album
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    摘要:

    为揭示中国橄榄（Canarium album）种质资源的遗传多样性，采用ISSR和RAPD标记对橄榄主要分布区的86份种质资源进行遗传多样性分析并构建核心种质。结果表明，基于UPGMA遗传相似系数，86份种质资源可分为3个大类；基于STRUCTURE模型聚类，可分为4个类群，这基本符合橄榄的地域性分布规律。采用ISSR和RAPD获得的中国橄榄种质资源的整体遗传多样性水平分别为0.284±0.169和0.244±0.163，多态性位点百分率分别为92.56%和100%，总遗传分化系数分别为0.127和0.142，基因流分别为3.423和3.025，群体间遗传相似系数分别为0.930和0.939，个体间遗传相似系数分别为0.736和0.732。因此，中国橄榄种质资源丰富的遗传多样性主要来源于个体间的遗传分化或变异，且这种遗传多样性存在明显的地域性差异。

    Abstract:

    To reveal the genetic diversity of germplasm resources of Canarium album in China, the genetic diversity of 86 germplasms selected from the major distribution areas was studied by using ISSR and RAPD markers, and core germplasms were constructed. The results showed that 86 germplasms could be divided into 3 groups based on UPGMA genetic similarity, and 4 groups by using STRUCTURE cluster analysis, which basically conform to the regional distribution of C. album in China. The overall genetic diversity coefficient using ISSR and RAPD was 0.284±0.169 and 0.244±0.163, the percentage of polymorphism loci was 92.56% and 100%, the genetic differentiation coefficient was 0.127 and 0.142, the gene flow was 3.423 and 3.025, the average genetic similarity coefficient between populations was 0.930 and 0.939, and the average genetic similarity coefficient between individuals was 0.736 and 0.732. Therefore, it was suggested that the rich genetic diversity of C. album in China mainly caused by the genetic differentiation or variation among individuals, and there were obvious regional differences.

    参考文献
    [1] CHANG Q, SU M H, CHEN Q X, et al. Physicochemical properties and antioxidant capacity of Chinese olive (Canarium album L.) cultivars[J]. J Food Sci, 2017, 82(6):1369-1377. doi:10.1111/1750-3841.13740.
    [2] ZENG H L, MIAO S, ZHENG B D, et al. Molecular structural characteristics of polysaccharide fractions from Canariumalbum (Lour.) Raeusch and their antioxidant activities[J]. J Food Sci, 2015, 80(11):H2585-H2596. doi:10.1111/1750-3841.13076.
    [3] CHEN F Z, YANG L P, HUANG Y N, et al. Isocorilagin, isolated from Canariumalbum (Lour.) Raeusch, as a potent neuraminidase inhibitor against influenza A virus[J]. Biochem Biophys Res Commun, 2020, 523(1):183-189. doi:10.1016/j.bbrc.2019.12.043.
    [4] KUO Y H, YEH Y T, PAN S Y, et al. Identification and structural elucidation of anti-inflammatory compounds from Chinese olive (Canariumalbum L.) fruit extracts[J]. Foods, 2019, 8(10):441. doi:10.3390/foods8100441.
    [5] YEH Y T, CHIANG A N, HSIEH S C. Chinese olive (Canariumalbum L.) fruit extract attenuates metabolic dysfunction in diabetic rats[J]. Nutrients, 2017, 9(10):1123. doi:10.3390/nu9101123.
    [6] LAI R L, CHEN J, FENG X, et al. Advances on germplasm resource evaluation and cold resistance research in Chinese olive of China[J]. Chin J Trop Crop, 2017, 38(11):2188-2194. doi:10.3969/j.issn.1000-2561.2017.11.031. 赖瑞联, 陈瑾, 冯新, 等. 中国橄榄种质资源评价与抗寒性研究进展[J]. 热带作物学报, 2017, 38(11):2188-2194. doi:10.3969/j.issn. 1000-2561.2017.11.031.
    [7] WANG M, PENG J Q, CAO J W, et al. Studies on genetic diversity of 48 wild species of magnoliaceae in Yunnan[J]. J Trop Subtrop Bot, 2020, 28(3):277-284. doi:10.11926/jtsb.4161. 王猛, 彭继庆, 曹基武, 等. 云南木兰科48种野生植物资源的遗传多样性研究[J]. 热带亚热带植物学报, 2020, 28(3):277-284. doi:10.11926/jtsb.4161.
    [8] PAN D R, ZENG H Y, CHEN G S, et al. Genetic diversity of chewing cane germplasms using RAPD and ISSR markers[J]. J Trop Subtrop Bot, 2007, 15(3):183-190. doi:10.3969/j.issn.1005-3395.2007.03.001. 潘大仁, 曾惠阳, 陈观水, 等. RAPD和ISSR分子标记对果蔗种质资源的遗传多样性研究[J]. 热带亚热带植物学报, 2007, 15(3):183-190. doi:10.3969/j.issn.1005-3395.2007.03.001.
    [9] YANG P K, ZHUANG D H, MA R J. Establishment of ISSR system for Canariumalbum using orthogonally designed experiments[J]. S China Fruits, 2010, 39(2):31-33. doi:10.13938/j.issn.1007-1431.2010. 02.020. 杨培奎, 庄东红, 马瑞君. 利用正交试验建立橄榄的ISSR-PCR反应体系[J]. 中国南方果树, 2010, 39(2):31-33. doi:10.13938/j.issn. 1007-1431.2010.02.020.
    [10] LIU T L, PAN D M, XU C T, et al. Optimization of ISSR-PCR reaction system in olive[J]. Biotechnol Bull, 2010(7):137-141. doi:10.13560/j. cnki.biotech.bull.1985.2010.07.033. 刘天亮, 潘东明, 许长同, 等. 橄榄ISSR-PCR反应体系的优化[J]. 生物技术通报, 2010(7):137-141. doi:10.13560/j.cnki.biotech.bull. 1985.2010.07.033.
    [11] NIE Z S, LAI Z X, PAN D M, et al. Genomic DNA extraction and optimization of RAPD amplifying conditions of Canarium album reausch[J]. Subtrop Agric Res, 2005, 1(2):6-8. doi:10.3969/j.issn. 1673-0925.2005.02.002. 聂珍素, 赖钟雄, 潘东明, 等. 橄榄基因组DNA提取及RAPD扩增条件优化[J]. 亚热带农业研究, 2005, 1(2):6-8. doi:10.3969/j.issn. 1673-0925.2005.02.002.
    [12] MEI Z Q, YANG L Q, ZHANG T D, et al. Improved RAPD analysis of Canarium album (Lour.) Raeusch from Sichuan Province along Yangtze Rriver in China[J]. Annu Res Rev Biol, 2014, 4(1):51-60. doi:10. 9734/ARRB/2014/5015.
    [13] MEI Z L, ZHANG X Q, LIU X Y, et al. Genetic analysis of Canarium album in different areas of China by improved RAPD and ISSR[J]. C R Biol, 2017, 340(11/12):558-564. doi:10.1016/j.crvi.2017.09.006.
    [14] CHENG J L, YIN Z C, MEI Z Q, et al. Development and significance of SCAR marker QG12-5 for Canarium album (Lour.) Raeusch by molecular cloning from improved RAPD amplification[J]. Genet Mol Res, 2016, 15(3):15038347. doi:10.4238/gmr.15038347.
    [15] YANG P K, ZHENG D X, MA R J, et al. Genetic diversity analysis of Canariumalbum L. landrances in Chaoshan area by ISSR[J]. Guang-dong Agric Sci, 2013, 40(23):129-132. doi:10.3969/j.issn.1004-874X. 2013.23.031. 杨培奎, 郑道序, 马瑞君, 等. 潮汕橄榄地方品种(系)遗传多样性的ISSR分析[J]. 广东农业科学, 2013, 40(23):129-132. doi:10.3969/j. issn.1004-874X.2013.23.031.
    [16] YANG P K, ZHUANG D H, MA R J, et al. The ISSR analysis of genetic diversity of Canariumalbum L. germplasm resources in eastern Guangdong[J]. Chin Agric Sci Bull, 2011, 27(24):86-92. 杨培奎, 庄东红, 马瑞君, 等. 粤东地区橄榄种质资源遗传多样性的ISSR分析[J]. 中国农学通报, 2011, 27(24):86-92.
    [17] LIU T L. ISSR analysis of germplasm resources of Fujian Canaviumalbum Raeusch[D]. Fuzhou:Fujian Agriculture and Forestry University, 2010:14-42. 刘天亮. 福建省橄榄(Canaviumalbum Raeusch)种质资源的ISSR分析[D]. 福州:福建农林大学, 2010:14-42.
    [18] CHEN M X, ZHANG Q J, CHEN Q X, et al. ISSR analysis of main cultivated and fresh-eating Canarium album (Lour.) Raeusch in Fuzhou City[J]. Mod Agric Sci Technol, 2011(21):129-130,133. doi:10.3969/j.issn.1007-5739.2011.21.078. 陈明贤, 张庆举, 陈清西, 等. 福州市主栽橄榄品种与若干鲜食橄榄优株的ISSR分析[J]. 现代农业科技, 2011(21):129-130,133. doi:10.3969/j.issn.1007-5739.2011.21.078.
    [19] NIE Z S. RAPD analysis of album (Canariumalbum) genetic resources in Fujian Province[D]. Fuzhou:Fujian Agriculture and Forestry University, 2005:24-48. 聂珍素. 福建橄榄(Canariumalbum L.)资源的RAPD分析[D]. 福州:福建农林大学, 2005:24-48.
    [20] WANG Y P, CHEN Q, ZHOU Z Q, et al. Optimization of AFLP reaction system in Canarium album[J]. J Fruit Sci, 2012, 29(3):505-511. doi:10.13925/j.cnki.gsxb.2012.03.033. 王燕平, 陈勤, 周志钦, 等. 橄榄基因组AFLP扩增体系的优化[J]. 果树学报, 2012, 29(3):505-511. doi:10.13925/j.cnki.gsxb.2012.03.033.
    [21] ZHOU J H, WANG Y P, CHEN Q, et al. Genetic diversity analysis with AFLP on fresh Chinese olive cultivars resources in Guangdong Province[J]. Guihaia, 2014, 34(1):4-9. doi:10.3969/j.issn.1000-3142. 2014.01.002. 周俊辉, 王燕平, 陈勤, 等. 用AFLP分析广东省鲜食橄榄品种资源遗传多样性[J]. 广西植物, 2014, 34(1):4-9. doi:10.3969/j.issn. 1000-3142.2014.01.002.
    [22] ODONG T L, JANSEN J, VAN EEUWIJK F A, et al. Quality of core collections for effective utilization of genetic resources:Review, discussion and interpretation[J]. Theor Appl Genet, 2013, 126(2):289-305. doi:10.1007/s00122-012-1971-y.
    [23] YONEZAWA K, NOMURA T, MORISHIMA H. Sampling strategies for use in stratified germplasm collections[M]//HODGKIN T, BROWN A H D, VAN HINTUM T J L, et al. Core Collections of Plant Genetic Resources. Chichester, UK:John Wiley & Sons, 1995:35-53.
    [24] YANG P K, ZHUANG D H, MA R J. Studies on sampling schemes of core collection of Canariumalbum L. in eastern Guangdong, China[J]. J Trop Subtrop Bot, 2012, 20(3):277-284. doi:10.3969/j.issn.1005-3395.2012.03.011. 杨培奎, 庄东红, 马瑞君. 粤东橄榄资源核心种质取样方案的研究[J]. 热带亚热带植物学报, 2012, 20(3):277-284. doi:10.3969/j.issn. 1005-3395.2012.03.011.
    [25] CHEN R Q. Analysis of genetic diversity of Chinese white olive (Canarium album) in Fujian Province using 18S-26SrDNA-PCR-ITS[D]. Fuzhou:Fujian Agriculture and Forestry University, 2005:12-24. 陈瑞庆. 福建橄榄(Canarium album)遗传多态性上的研究[D]. 福州:福建农林大学, 2005:12-24.
    [26] EVANNO G, REGNAUT S, GOUDET J. Detecting the number of clusters of individuals using the software STRUCTURE:A simulation study[J]. Mol Ecol, 2005, 14(8):2611-2620. doi:10.1111/j.1365-294X. 2005.02553.x.
    [27] YANG P K, ZHUANG D H, MA R J. Application of ITS sequences in the identification of local variety resources of olive[J]. S China Fruits, 2015, 44(2):23-26. doi:10.13938/j.issn.1007-1431.20140543. 杨培奎, 庄东红, 马瑞君. ITS序列在橄榄地方品种资源鉴定中的应用[J]. 中国南方果树, 2015, 44(2):23-26. doi:10.13938/j.issn. 1007-1431.20140543.
    [28] NYBOM H. Comparison of different nuclear DNA markers for esti-mating intraspecific genetic diversity in plants[J]. Mol Ecol, 2004, 13(5):1143-1155. doi:10.1111/j.1365-294X.2004.02141.x.
    [29] WRIGHT S. Evolution and the Genetics of Populations. A Treatise in Four Volumes. Volume 4. Variability within and Among Natural Popu-lations[M]. Chicago, London:University of Chicago Press, 1978.
    [30] IRWIN E D, BENSCH S, IRWIN J H, et al. Speciation by distance in a ring species[J]. Science, 2005, 307(5708):414-416. doi:10.1126/science.1105201.
    [31] JANSEN J, VAN HINTUM T. Genetic distance sampling:A novel sampling method for obtaining core collections using genetic distances with an application to cultivated lettuce[J]. Theor Appl Genet, 2007, 114(3):421-428. doi:10.1007/s00122-006-0433-9.
    [32] WAN J F, WU R J, WEI X X, et al. Diversity analysis of the leaf phenotypic characters of Chinese olive germplasm resources[J]. Chin J Trop Crop, 2012, 33(6):975-980. doi:10.3969/j.issn.1000-2561.2012. 06.002. 万继锋, 吴如健, 韦晓霞, 等. 橄榄种质资源叶片表型性状多样性分析[J]. 热带作物学报, 2012, 33(6):975-980. doi:10.3969/j.issn. 1000-2561.2012.06.002.
    [33] WEI X X, LAI R L, CHEN J, et al. Studies on genetic diversity on inflorescence phenotypic characteristics of Canariumalbum germ-plasm resource[J]. J Trop Subtrop Bot, 2019, 27(1):1-10. doi:10. 11926/jtsb.3940. 韦晓霞, 赖瑞联, 陈瑾, 等. 橄榄种质资源花序表型性状遗传多样性研究[J]. 热带亚热带植物学报, 2019, 27(1):1-10. doi:10.11926/jtsb.3940.
    [34] WU R J, WAN J F, WEI X X, et al. Fruit character diversity analysis and numerical classification of Chinese olive germplasm resources[J]. J Fruit Sci, 2015, 32(5):797-805. doi:10.13925/j.cnki.gsxb. 20150129. 吴如健, 万继锋, 韦晓霞, 等. 橄榄种质资源果实表型性状多样性分析及其数量分类研究[J]. 果树学报, 2015, 32(5):797-805. doi:10.13925/j.cnki.gsxb.20150129.
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赖瑞联,陈瑾,冯新,韦晓霞,陈义挺,沈朝贵,吴如健.橄榄ISSR和RAPD遗传多样性分析和核心种质构建[J].热带亚热带植物学报,2022,30(1):41~53

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  • 收稿日期:2021-04-29
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