基于SSR标记的广东栽培益智群体遗传多样性分析

Genetic Diversity of Cultivated Populations of Alpinia oxyphylla in Guangdong Based on SSR Marker

摘要:

为了解广东栽培益智(Alpinia oxyphylla)群体的遗传多样性，采用SSR分子标记技术对166份种质的遗传差异进行研究。结果表明，14对SSR引物共检测到88个等位基因，每对引物检测的有效等位基因为1.198~3.279，平均2.599；Shannon多样性信息指数为0.736~1.890，平均1.107。方差分析表明20.87%的变异来自组间，79.13%的变异来自组内。基于主成分分析和遗传结构分析表明，供试166份益智样本可分为4大类群，但没有反映出形态特征的规律性。因此，益智种质资源具有较高遗传多样性，且遗传变异主要发生在群体内，群体间的遗传分化较低，且基于表型性状的类型划分和基于SSR分子标记的聚类未能实现一致性。

Abstract:

In order to understand the genetic diversity of Alpinia oxyphylla in Guangdong, the genetic differences among 166 germplasms were studied by using SSR molecular markers. The results showed that a total of 88 alleles were detected by 14 pairs of SSR primers, and the effective alleles of each primer varied from 1.198 to 3.279 with an average of 2.599. The Shannon's information index ranged from 0.736 to 1.890 with an average of 1.107. The variance analysis showed that 20.87% of the variation came between groups. Based on principal component analysis and genetic structure analysis, the 166 germplasms could be divided into four groups, which did not show the regularity of morphological characteristics. Therefore, there were high genetic diversity mainly occurred within population, but the classification based on morphological traits and the clustering based on SSR molecular markers could not achieve the consistency.