NAA处理对艾纳香扦插生根生理生化特性的影响

Effects of NAA on Physiological and Biochemical Characteristic of Blumea balsamifera during Cutting Rooting

摘要:

为探讨NAA对艾纳香（Blumea balsamifera）扦插生根的影响，4 a生艾纳香健康枝条用500 mg/L NAA处理，对生根过程中的生理生化特征进行了研究。结果表明，艾纳香扦插生根率与内源IAA、GA含量和IAA/ABA呈正相关，而与ABA含量呈负相关。NAA处理能提高插穗的IAA含量，降低ABA含量，有助于不定根的产生。插穗生根率与多酚氧化酶（PPO）活性正相关，与吲哚乙酸氧化酶（IAAO）活性负相关。NAA处理能够提高插穗PPO活性，降低IAAO活性，对插穗生根有利。可溶性蛋白、可溶性总糖含量与生根率均呈正相关关系。NAA处理可促进插穗的可溶性总糖、可溶性蛋白的积累，提高插穗的细胞分化和代谢能力。因此，500 mg/L NAA处理插穗可促进不定根的生长发育。

Abstract:

In order to explore the effect of NAA on cutting rooting of Blumea balsamifera, the changes in physiological and biochemical characteristics of 4-year-old healthy branches as cuttings were studied treated with 500 mg/L NAA. The results showed that rooting rate was positively correlated with endogenous IAA, GA content and IAA/ABA, but negatively with ABA content. The IAA content of cuttings increased and ABA content decreased treated with NAA, showing that NAA had helped with adventitious roots. The rooting rate of cuttings was positively correlated with PPO activity and negatively with IAAO activity, and NAA could enhance PPO activity and reduce IAAO activity of cuttings, which is beneficial to rooting. The contents of soluble protein and total soluble sugar were positively correlated with rooting rate. NAA could promote the accumulation of total soluble sugar and soluble protein, improve the cell differentiation and metabolic ability of cuttings. Therefore, 500 mg/L NAA could promote the growth and development of adventitious roots.