黄兰叶乙醇提取物的活性研究和成分分析

Bioactivity and Chemical Composition of Ethanol Extract from Michelia champaca Leaves

摘要:

为挖掘黄兰（Michelia champaca）叶的开发潜力，采用抗氧化评价、细胞评价和仪器分析方法对超声提取的黄兰叶乙醇提取物进行活性研究和化学成分的定性分析。DPPH·自由基清除和铁离子还原力测定结果表明，黄兰叶的20%和80%乙醇提取物具有抗氧化潜能。黄兰叶20%乙醇提取物的质量浓度超过12.5 μg/mL，80%乙醇提取物的质量浓度超过6.25 μg/mL，对HacaT细胞具有细胞毒性。采用超高效液相-质谱联用方法结合软件谱库搜索对黄兰叶乙醇提取物进行成分分析，共检索到206个化合物，包括黄酮、生物碱、脂肪酸、醇等多种类型，通过标准品验证可明确鉴定出芦丁、槲皮素、山奈酚等黄酮类物质。不同月份的黄兰叶乙醇提取物的成分含量不相同，成分分布在春夏季与秋冬季存在明显差异，某些成分具有季节独特性。因此，黄兰叶在日化产品开发上存在潜力，可结合实际根据成分选择采收时间。

Abstract:

In order to excavate the development and utilization potential of Michelia champaca leaves, the bioactivity and chemical composition of ethanol extract from leaves obtained by ultrasonic extraction were studied. The results showed that 20% and 80% ethanol extracts had antioxidant potential ability by using DPPH∙ free radical scavenging assay and reducing power of iron ion. There was cytotoxicity to HacaT cells, when the concentration of 20% or 80% extracts was more than 12.5 or 6.25 μg/mL, respectively. A total of 206 compounds were obtained from ethanol extract from M. champaca leaves by UPLC-MS combined with software spectrum library, including flavonoids, alkaloids, fatty acids, alcohols and so on. Among the flavonoids, rutin, quercetin and kaempferol could be identified clearly by standard product verification. The contents of compounds in ethanol extracts of M. champaca leaves were different among different months, and the distribution of components in spring and summer were significantly different from that in autumn and winter. Besides, some compositions had seasonal characteristics. In conclusion, Michelia champaca leaves had potential in the development of daily chemical products, and the harvest time could be selected according to the actual composition.