东乡野生稻苗期响应低温胁迫的转录组分析

Transcriptome Analysis of Response to Low Temperature Stress in Dongxiang Wild Rice at Seedling Stage

摘要:

为了解东乡野生稻（Oryza rufipogon）对低温胁迫的响应机制，对苗期的RNA-seq转录表达谱进行了研究。结果表明，与对照相比，共检测到10 200个差异表达基因（DEGs），其中5 201个上调表达，4 999个下调表达，其中有426个DEGs位于已报道的水稻耐冷QTL区间，且37个为耐冷调控相关的家族基因。GO功能分类和KEGG代谢路径分析表明，核酸结合转录因子活性、氨基酸生物合成以及光合作用代谢等均参与响应低温胁迫过程。实时荧光定量分析表明，ABA响应蛋白基因、MYB转录因子和40S核糖体蛋白SA基因等12个可能与低温胁迫响应相关的DEGs表达模式与RNA-seq的一致。可见，植物激素传导途径和转录因子相关调控基因在东乡野生稻苗期响应低温胁迫过程中起重要作用。

Abstract:

In order to study the response mechanism of Dongxiang wild rice (Oryza rufipogon) to low temperature stress, its RNA-Seq transcriptional expression profile at seedling stage was studied. The results showed that a total of 10 200 differentially expressed genes (DEGs) under low temperature stress were detected compared with control under normal temperature, among which 5 201 DEGs were up-regulated and 4 999 were down-regulated. There were 426 DEGs located in the reported QTL interval of cold tolerance in rice, and 37 of them were family genes related to cold tolerance regulation. Go functional classification and KEGG metabolic pathway analysis of DEGs indicated that nucleic acid binding transcription factor activity, amino acid biosynthesis and photosynthetic metabolism were involved in the response to low temperature stress. The expression patterns of 12 DEGs, including ABA response protein gene, MYB transcription factor and 40S ribosomal protein SA gene, might be related to low temperature stress response by real-time fluorescence quantitative PCR analysis were consistent with those of RNA-Seq. So, it was suggested that plant hormone transduction pathways and transcription factor-related regulatory genes could play an important role in response to low temperature stress in Dongxiang wild rice seedling stage.