秋石斛花青素提取液成分分析及其体外抗氧化活性和刺激性研究

Component Analysis of Dendrobium phalaenopsis Anthocyanin Extract and Its Antioxidant Activity and Irritation in vitro

摘要:

为了解热带兰秋石斛花青素的功能，用超声波辅助提取法提取秋石斛‘三亚阳光’（Dendrobium phalaenopsis ‘Sanya Sunny’）的花青素，用UPLC-QTOF-MS技术对其花青素提取液进行成分分析，并对其抗氧化活性进行了评价，采用家兔皮肤刺激和眼刺激实验对提取物的安全性进行了研究。结果表明，从花青素提取物中共检测出15个花青素成分，如苷元有飞燕草素、矢车菊素、锦葵素、矮牵牛素和芍药素等；提取物体外抗氧化活性较强，相同浓度下花青素提取物的DPPH·清除能力和总抗氧化能力均强于抗坏血酸；0.25~1.0 g/L的花青素提取物对家兔眼和皮肤均无刺激性。这说明‘三亚阳光’秋石斛除观赏价值外，在医药、食品和化妆品等领域也有广阔的应用空间。

Abstract:

In order to understand the function of anthocyanin from tropical orchid Dendrobium phalaenopsis, the anthocyanin of D. phalaenopsis ‘Sanya Sunny’ was extracted by ultrasonic-assisted method, the constituents of anthocyanin extract was analyzed by UPLC-QTOF-MS. The total antioxidant capacity (FRAP) and the scavenging capacity of diphenyl picroacyl (DPPH·) of the extract were determined, and ascorbic acid was used as a positive control to evaluate its antioxidant capacity. Rabbit skin irritation and eye irritation experiments were used to evaluate the irritation of extract. The results showed that a total of 15 anthocyanins were detected in the extract, such as aglycones include delphinium pigment, cyanidin, mallow pigment, petunia pigment and peony pigment. The extract has strong antioxidant activity in vitro. The scavenging ability of DPPH· and total antioxidant ability are both stronger than ascorbic acid at the same concentration. There were not irritation on rabbit eyes and skin treated with 0.25-1.0 g/L extract. Therefore, it was suggested that besides ornamental value, Dendrobium phalaenopsis ‘Sanya Sunny’ also had broad application space in medicine, food and cosmetics fields.