Abstract:Two newly naturalized species of Asteraceae, Acmella brachyglossa Cass. and Emilia praetermissa Milne-Redh. in Mainland China were reported. The former is native to Central and South America and the Caribbean, and naturalized in Taiwan. Recently a population of this species was found in Huangpu District of Guangzhou City. The previously reported new record of this species in Xiangshan County, Zhejiang Province was actually a misidentification of A. radicans var. debilis (Kunth) R. K. Jansen. Emilia praetermissa originates in West Africa, which also becomes naturalized in Taiwan. Many populations of this invasive species were discovered in Conghua District of Guangzhou City and Heshan City this year. The vouchers are housed in Herbarium of South China Botanical Garden, Chinese Academy of Sciences (IBSC).