云南巍山地区广义虫草的物种多样性研究

Species Diversity of Cordyceps Sensu Lato in Weishan, Yunnan

摘要:

为了解云南巍山地区的广义虫草资源状况，通过资源调查，利用形态观察结合多基因（nrSSU、nrLSU、EF-1α、RPB1和RPB2）系统发育分析，对广义虫草的物种多样性进行了研究。结果表明，巍山地区共采集虫草341株，隶属于2科5属20种，其中有7种为疑似新物种，虫草寄主包含鳞翅目、鞘翅目、膜翅目和半翅目昆虫。巍山地区虫草优势属为虫草属（Cordyceps,7种215株），优势种为蛹虫草（C.militaris,93株）。巍山地区北部红河源样地的虫草物种多样性和丰富度最高,小鸡足山样地最低。物种累计曲线表明巍山地区绝大多数虫草种类已被采集研究。这为云南虫草资源保护与可持续利用提供依据。

Abstract:

In order to reveal the status of Cordyceps sensu lato resource in Weishan County, Yunnan Province, the species diversity of Cordyceps sensu lato was studied based on field survey, morphological observation and multigene (nrSSU, nrLSU, EF1-α, RPB1 and RPB2) phylogenetic analysis. The results showed that there were 341 strains of Cordyceps sensu lato collected from 5 plots, belonging to two families and five genera, including seven suspected new species. The hosts of Cordyceps sensu lato were Lepidoptera, Coleoptera, Hymenoptera and Dermaptera, respectively. The dominant genus was Cordyceps collected 7 species 215 strains, and C. militaris was dominant species collected 93 strains. The species diversity and richness of Cordyceps sensu lato were the highest in plot of Red River Source and the lowest in plot of Xiao Jizu Shan. Based on the accumulative curve, it was indicated that most of Cordyceps sensu lato had been collected in Weishan area. So, these would provide the basis for protection and sustainable utilization of Cordyceps sensu lato in Yunnan Province.