云南巍山地区广义虫草的物种多样性研究
作者:
基金项目:

国家自然科学基金面上项目(31870017);云南省科技厅项目[2018FY001(-006)]资助


Species Diversity of Cordyceps Sensu Lato in Weishan, Yunnan
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [30]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为了解云南巍山地区的广义虫草资源状况,通过资源调查,利用形态观察结合多基因(nrSSUnrLSUEF-1αRPB1RPB2)系统发育分析,对广义虫草的物种多样性进行了研究。结果表明,巍山地区共采集虫草341株,隶属于2科5属20种,其中有7种为疑似新物种,虫草寄主包含鳞翅目、鞘翅目、膜翅目和半翅目昆虫。巍山地区虫草优势属为虫草属(Cordyceps,7种215株),优势种为蛹虫草(C.militaris,93株)。巍山地区北部红河源样地的虫草物种多样性和丰富度最高,小鸡足山样地最低。物种累计曲线表明巍山地区绝大多数虫草种类已被采集研究。这为云南虫草资源保护与可持续利用提供依据。

    Abstract:

    In order to reveal the status of Cordyceps sensu lato resource in Weishan County, Yunnan Province, the species diversity of Cordyceps sensu lato was studied based on field survey, morphological observation and multigene (nrSSU, nrLSU, EF1-α, RPB1 and RPB2) phylogenetic analysis. The results showed that there were 341 strains of Cordyceps sensu lato collected from 5 plots, belonging to two families and five genera, including seven suspected new species. The hosts of Cordyceps sensu lato were Lepidoptera, Coleoptera, Hymenoptera and Dermaptera, respectively. The dominant genus was Cordyceps collected 7 species 215 strains, and C. militaris was dominant species collected 93 strains. The species diversity and richness of Cordyceps sensu lato were the highest in plot of Red River Source and the lowest in plot of Xiao Jizu Shan. Based on the accumulative curve, it was indicated that most of Cordyceps sensu lato had been collected in Weishan area. So, these would provide the basis for protection and sustainable utilization of Cordyceps sensu lato in Yunnan Province.

    参考文献
    [1] JIANG X, WANG C Y, LI L Z, et al. Activity comparison study on Paecilomyces hepiali, Cordyceps militaris and wild Cordyceps sinensis[J]. J Changchun Univ Trad Chin Med, 2017, 33(4):544-547. doi:10. 13463/j.cnki.cczyy.2017.04.010. 姜雪, 王春月, 李兰洲, 等. 蝙蝠蛾拟青霉及蛹虫草与野生冬虫夏草活性比较[J]. 长春中医药大学学报, 2017, 33(4):544-547. doi:10.13463/j.cnki.cczyy.2017.04.010.
    [2] LI Z Z. History, progress and current status of the application of fungi against pest insects in China[J]. Chin J Biol Control, 2015, 31(5):699-711. doi:10.16409/j.cnki.2095-039x.2015.05.010. 李增智. 我国利用真菌防治害虫的历史、进展及现状[J]. 中国生物防治学报, 2015, 31(5):699-711. doi:10.16409/j.cnki.2095-039x.2015. 05.010.
    [3] LIU Y J, ZHOU H B, DONG X R, et al. Species diversity and distribution characteristics of entomogenous fungi in Zipeng Mountain National Forest Park[J]. Chin J Biol Control, 2018, 34(4):568-573. doi:10.16409/j.cnki.2095-039x.2018.04.011. 刘玉军, 周海波, 董先茹, 等. 紫蓬山国家森林公园虫生真菌物种多样性与分布特点[J]. 中国生物防治学报, 2018, 34(4):568-573. doi:10.16409/j.cnki.2095-039x.2018.04.011.
    [4] WANG S B, HUANG Y P, FAN M Z, et al. Species diversity of entomogenous fungal flora of Mountains Dabie, Anhui[J]. Biodiv Sci, 2003, 11(6):475-479. doi:10.17520/biods.2003056. 王四宝, 黄勇平, 樊美珍, 等. 安徽大别山区虫生真菌区系的物种多样性研究[J]. 生物多样性, 2003, 11(6):475-479. doi:10.17520/biods.2003056.
    [5] SONG B, LI T H, WU X L, et al. Preliminary study of Cordyceps diversity in Yunnan, Guizhou and Guangxi Provinces, China[J]. Guizhou Sci, 2004, 22(4):41-44. 宋斌, 李泰辉, 吴兴亮, 等. 滇黔桂虫草资源多样性初步研究[J]. 贵州科学, 2004, 22(4):41-44.
    [6] TANG B H, MIAO W L, ZHANG Y Z. Study on Cordyceps resources in Henan Province[J]. J Anhui Agri Sci, 2008, 36(28):12311-12312, 12323. doi:10.3969/j.issn.0517-6611.2008.28.099. 唐保宏, 苗文莉, 张一折. 河南省虫草资源调查研究[J]. 安徽农业科学, 2008, 36(28):12311-12312,12323. doi:10.3969/j.issn.0517-6611. 2008.28.099.
    [7] PENG F, CAO S, ZHANG Q, et al. Investigation on resources of Cordyceps sensu lato in Wanfoshan Reserve[J]. J Anhui Agri Sci, 2011, 38(3):400-403. doi:10.13610/j.cnki.1672-352x.2011.03.019. 彭凡, 曹珊, 张琪, 等. 安徽省万佛山地区虫草资源的初步调查[J]. 安徽农业大学学报, 2011, 38(3):400-403. doi:10.13610/j.cnki.1672-352x.2011.03.019.
    [8] CHEN F J, XUE D J. Climate suitability zoning of walnut planting in Dali Bai Nationality Autonomous Prefecture[J]. J China W Norm Univ (Nat Sci), 2019, 40(4):407-412. doi:10.16246/j.issn.1673-5072.2019. 04.014. 陈凤娇, 薛东剑. 大理白族自治州核桃种植气候适宜性区划[J]. 西华师范大学学报(自然科学版), 2019, 40(4):407-412. doi:10. 16246/j.issn.1673-5072.2019.04.014.
    [9] ZHANG Y Z. Macrofungi resources in Weishan County[J]. Ed Fungi China, 1991, 10(1):28-29. doi:10.13629/j.cnki.53-1054.1991.01.012. 张一中. 巍山县大型真菌资源[J]. 中国食用菌, 1991, 10(1):28-29. doi:10.13629/j.cnki.53-1054.1991.01.012.
    [10] MA H L, XIONG J F, MA H, et al. Grassland resources and ecological status of Weishan County[J]. China Anim Ind, 2012(24):64-65. doi:10.3969/j.issn.2095-2473.2012.24.030. 马海轮, 熊景发, 马红, 等. 巍山县草原资源与生态状况[J]. 中国畜牧业, 2012(24):64-65. doi:10.3969/j.issn.2095-2473.2012.24.030.
    [11] WU Y H. Present status and management countermeasures of forest resources consumption in Weishan County[J]. For Invent Plann, 2017, 42(6):48-51. doi:10.3969/j.issn.1671-3168.2017.06.010. 吴永会. 巍山县森林资源消耗现状调查与管理对策[J]. 林业调查规划, 2017, 42(6):48-51. doi:10.3969/j.issn.1671-3168.2017.06.010.
    [12] LIANG Z Q, LIU A Y, HUANG J Z, et al. Some Cordyceps species and their allies from Suoluo Nature Preserve in Guizhou[J]. Mycosystema, 2002, 21(1):9-14. doi:10.3969/j.issn.1672-6472.2002.01.004. 梁宗琦, 刘爱英, 黄建忠, 等. 桫椤自然保护区的虫草及其相关真菌[J]. 菌物系统, 2002, 21(1):9-14. doi:10.3969/j.issn.1672-6472. 2002.01.004.
    [13] WANG Y B. Studies on phylogeny of Polycephalomycetaceae fam. nov., with microbial diversities of Polycephalomyces multiramosus and its host[D]. Kunming:Yunnan University, 2016:1-182. 王元兵. 多头霉新科系统发育研究——兼论多枝多头霉及其寄主微生物多样性[D]. 昆明:云南大学, 2016:1-182.
    [14] CHEN Z H, DAI Y D, YU H, et al. Systematic analyses of Ophiocordyceps lanpingensis sp. nov., a new species of Ophiocordyceps in China[J]. Microbiol Res, 2013, 168(8):525-532. doi:10.1016/j.micres.2013. 02.010.
    [15] SUNG G H, HYWEL-JONES N L, SUNG J M, et al. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi[J]. Stud Mycol, 2007, 57:5-59. doi:10.3114/sim.2007.57.01.
    [16] KEPLER R M, LUANGSA-ARD J J, HYWEL-JONES N L, et al. A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales)[J]. IMA Fungus, 2017, 8(2):335-353. doi:10.5598/imafungus.2017. 08.02.08.
    [17] DAI Y D, YU H, YANG K. Identifying a sporocarp-inhabiting fungus Isaria farinosa and analyzing its molecular phylogeny of ITS sequence[J]. Ed Fungi China, 2013, 32(5):46-52. doi:10.13629/j.cnki.53-1054. 2013.05.004. 代永东, 虞泓, 杨坤. 1株重寄生真菌粉棒束孢鉴定及系统发育分析[J]. 中国食用菌, 2013, 32(5):46-52. doi:10.13629/j.cnki.53-1054. 2013.05.004.
    [18] CHEN Z H, WANG Y B, DAI Y D, et al. Species diversity and seasonal fluctuation of entomogenous fungi of Ascomycota in Taibaoshan Forest Park in western Yunnan[J]. Biodiv Sci, 2019, 27(9):993-1001. doi:10.17520/biods.2019135. 陈自宏, 王元兵, 代永东, 等. 滇西太保山森林公园子囊菌门虫生真菌物种多样性及其消长动态[J]. 生物多样性, 2019, 27(9):993-1001. doi:10.17520/biods.2019135.
    [19] WANG Y B, NGUYEN T T, DAI Y D, et al. Molecular phylogeny and morphology of Ophiocordyceps unituberculata sp. nov. (Ophiocordy-cipitaceae), a pathogen of caterpillars (Noctuidae, Lepidoptera) from Yunnan, China[J]. Mycol Prog, 2018, 17(6):745-753. doi:10.1007/s11557-017-1370-5.
    [20] WANG Y B, YU H, DAI Y D, et al. Polycephalomyces yunnanensis (Hypocreales), a new species of Polycephalomyces parasitizing Ophiocordyceps nutans and stink bugs (hemipteran adults)[J]. Phytotaxa, 2015, 208(1):34-44. doi:10.11646/phytotaxa.208.1.3.
    [21] KONG F Z, YU R C, XU Z J, et al. Application of excel in calculation of biodiversity indices[J]. Marine Sci, 2012, 36(4):57-62. 孔凡洲, 于仁成, 徐子钧, 等. 应用Excel软件计算生物多样性指数[J]. 海洋科学, 2012, 36(4):57-62.
    [22] XU R J, HUANG C T, ZHOU X G, et al. Species diversity and dominate population dynamics of Triadica rotundifolia community in Qingxin and Yangshan Counties, Guangdong[J]. J Trop Subtrop Bot, 2013, 21(6):496-504. doi:10.3969/j.issn.1005-3395.2013.06.002. 徐瑞晶, 黄川腾, 周雪刚, 等. 清新和阳山县圆叶乌桕群落物种多样性和优势种群动态探讨[J]. 热带亚热带植物学报, 2013, 21(6):496-504. doi:10.3969/j.issn.1005-3395.2013.06.002.
    [23] HE B, LI Q, LIU Y. Community characteristics and species diversity of Pinus armandii in Caohai National Nature Reserve[J]. J Trop Subtrop Bot, 2020, 28(1):44-52. doi:10.11926/jtsb.4063. 何斌, 李青, 刘勇. 草海国家级自然保护区华山松群落特征及物种多样性研究[J]. 热带亚热带植物学报, 2020, 28(1):44-52. doi:10. 11926/jtsb.4063.
    [24] ZANG W, LIU C Y, XIE G F, et al. Resources and species diversity of the phyllosphere yeasts from the Yandang Mountains in Zhejiang Province[J]. Acta Ecol Sin, 2018, 38(11):3920-3930. doi:10.5846/stxb201705040827. 臧威, 刘春月, 谢广发, 等. 浙江雁荡山山脉叶栖酵母菌资源与物种多样性[J]. 生态学报, 2018, 38(11):3920-3930. doi:10.5846/stxb 201705040827.
    [25] LI Q. Species accumulation curves and its application[J]. Chin J Appl Entomol, 2011, 48(6):1882-1888. 李巧. 物种累积曲线及其应用[J]. 应用昆虫学报, 2011, 48(6):1882-1888.
    [26] ZHANG Q D, WEI W, CHEN L D, et al. Spatial variation of soil moisture and species diversity patterns along a precipitation gradient in the grasslands of the Loess Plateau[J]. J Nat Resour, 2018, 33(8):1351-1362. doi:10.31497/zrzyxb.20170726. 张钦弟, 卫伟, 陈利顶, 等. 黄土高原草地土壤水分和物种多样性沿降水梯度的分布格局[J]. 自然资源学报, 2018, 33(8):1351-1362. doi:10.31497/zrzyxb.20170726.
    [27] ZUO J H, GONG X Y, DONG Y M, et al. Research achievements in bioactive components, pharmacological effects and applications of Cordyceps militaris [J]. Food Sci, 2018, 39(21):330-339. 左锦辉, 贡晓燕, 董银卯, 等. 蛹虫草的活性成分和药理作用及其应用研究进展[J]. 食品科学, 2018, 39(21):330-339.
    [28] CHAI J P, RI X R, XIE D Y. Distribution and ecological factors of Cordyceps militaris in Yunnan[J]. Yunnan Agric Sci Technol, 2002(4):25. doi:10.3969/j.issn.1000-0488.2002.04.008. 柴建萍, 日兴荣, 谢道燕. 云南蛹虫草分布及发生生态因素浅析[J]. 云南农业科技, 2002(4):25. doi:10.3969/j.issn.1000-0488.2002. 04.008.
    [29] LIANG Z Q. Flora Fungorum Sinicorum, Vol. 32Cordyceps[M]. Beijing:Science Press, 2007:1-190. 梁宗琦. 中国真菌志, 第32卷虫草属[M]. 北京:科学出版社, 2007:1-190.
    [30] YANG Z L, YU H, CHEN Z H, et al. Study on the biological and ecological habits of populations of Cordyceps militaris in middle of Yunnan[J]. Ed Fungi China, 2011, 30(5):43-47. doi:10.3969/j.issn. 1003-8310.2011.05.016. 杨钟林, 虞泓, 陈自宏, 等. 滇中蛹虫草居群生物学及其生态学习性研究[J]. 中国食用菌, 2011, 30(5):43-47. doi:10.3969/j.issn. 1003-8310.2011.05.016.
    引证文献
引用本文

赵志远,王元兵,王志勤,汤德相,耿宇鹏,虞泓.云南巍山地区广义虫草的物种多样性研究[J].热带亚热带植物学报,2020,28(5):455~462

复制
分享
文章指标
  • 点击次数:533
  • 下载次数: 619
  • HTML阅读次数: 952
  • 引用次数: 0
历史
  • 收稿日期:2020-01-14
  • 最后修改日期:2020-03-30
  • 在线发布日期: 2020-09-18
文章二维码