首页 > 过刊浏览>2020年第28卷第4期 >356-366. DOI:10.11926/jtsb.4185
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基于表型性状和SSR标记的57份辣椒种质遗传多样性分析
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国家重点研发计划项目（2017YFD0101901）；国家大宗蔬菜产业技术体系合肥综合实验站项目（CARS-23-G39）；安徽省农业科学院创新团队项目（2020YL026）；安徽省重点科技攻关面上项目（1704g07020111）资助


Genetic Diversity Analysis of 57 Germplasms of Capsicum annuum Based on Phenotypic Traits and SSR Markers
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    摘要:

    为了解辣椒（Capsicum annuum）种质资源的遗传多样性，以57份辣椒种质资源为材料，对34个表型性状进行变异度、多样性及主成分分析，分别基于表型性状和SSR分子标记进行聚类分析。结果表明，种质间的34个表型性状存在差异，平均变异系数为40.67%，平均Shannon-Weiner多样性指数为1.20；提取的10个主成分可以代表辣椒种质表型性状75.972%的遗传信息，其中第1主成分占比22.317%，主要由果实横径、单果重、果肩形状和果顶性状所组成；19对SSR引物的平均Nei's基因多样性指数及香农信息指数分别为0.48和0.80；基于表型性状和SSR标记均将57份辣椒分为4类，但2种聚类结果间的相关性不显著（r=-0.175 9）。这为辣椒育种的亲本选配及种质资源评价提供理论依据。

    Abstract:

    In order to understand the genetic diversity of Capsicum annuum germplasms, the coefficient of variation, diversity and principal components analysis of 34 phenotypic traits of 57 germplasms were studied, and the cluster analysis of them based on phenotypic traits and SSR markers were carried out, respectively. The results showed that there were significant differences in 34 phenotypic traits among Capsicum germplasms, the mean coefficient of variation and Shannon-Weiner diversity index were 40.67% and 1.20, respectively, and the 10 principal components could represent 75.972% of genetic information of phenotypic traits, the first principal component accounted for 22.317%, which was mainly composed of fruit transverse diameter, single fruit weight, shoulder shape and top character; the average Nei's genetic diversity index and Shannon information index of 19 SSR primers were 0.48 and 0.80, respectively. Based on phenotypic traits and SSR markers, fifty-seven Capsicum germplasms could be divided into 4 groups, but the correlation between two clusters was not significant (r=-0.175 9). So, these would provide theoretical basis for selection of parents and evaluation of germplasm resources in Capsicum breeding.

    参考文献
    [1] GAO J X, WU X M, YANG A R, et al. Low temperature resistance evaluation of pepper germplasm at germination stage[J]. N Hort, 2018(9):19-24. doi:10.11937/bfyy.20174034. 高晶霞, 吴雪梅, 杨爱荣, 等. 辣椒不同种质资源种子萌发期耐低温性评价[J]. 北方园艺, 2018(9):19-24. doi:10.11937/bfyy.20174034.
    [2] SHAO Y J, WU W W, SHEN X X, et al. Genetic study on genic male sterility of pepper and its application in hybrid breeding[J]. J Trop Subtrop Bot, 2013, 21(1):93-99. doi:10.3969/j.issn.1005-3395.2013. 01.014. 邵元健, 吴雯雯, 沈素香, 等. 辣椒雄性核不育基因的遗传研究及其在杂交育种中的应用[J]. 热带亚热带植物学报, 2013, 21(1):93-99. doi:10.3969/j.issn.1005-3395.2013.01.014.
    [3] ZHANG B X, WANG L H, MAO S L, et al. Research progress on pepper genetic breeding during China's ‘Eleventh Five-year Plan’[J]. China Veget, 2010(24):1-9. 张宝玺, 王立浩, 毛胜利, 等. "十一五"我国辣椒遗传育种研究进展[J]. 中国蔬菜, 2010(24):1-9.
    [4] CHU P P, XIANG C P, ZHANG C X, et al. Genetic diversity of Cucurbita moschata genotypes revealed by RAPD markers and agro-nomic traits[J]. J Nucl Agric Sci, 2007, 21(5):441-446. doi:10.3969/j.issn.1000-8551.2007.05.004. 褚盼盼, 向长萍, 张称心, 等. 中国南瓜种质资源农艺性状与RAPD标记分析[J]. 核农学报, 2007, 21(5):441-446. doi:10.3969/j. issn.1000-8551.2007.05.004.
    [5] SHI J L, CHEN X Z, HUANG Z A, et al. Genetic diversity evaluation on main agronomic traits in south China ecotypic cucumber[J]. J Nucl Agric Sci, 2016, 30(10):1914-1924. doi:10.11869/j.issn.100-8551. 2016.10.1914. 史建磊, 陈先知, 黄宗安, 等. 华南型黄瓜主要农艺性状遗传多样性评价[J]. 核农学报, 2016, 30(10):1914-1924. doi:10.11869/j.issn. 100-8551.2016.10.1914.
    [6] WEBER J L, MAY P E. Abundant class of human DNA polymer-phisms which can be typed using the polymerase chain reaction[J]. Amer J Human Genet, 1989, 44(3):388-396.
    [7] YUAN W, ZHAO Y L, WANG L J. Development and application of SSR markers in peanut germplasm identification[J]. Mol Plant Breed, 2016, 14(12):3462-3468. doi:10.13271/j.mpb.014.003462. 苑旺, 赵永亮, 王丽洁. SSR分子标记在花生种质资源鉴定方面的应用研究[J]. 分子植物育种, 2016, 14(12):3462-3468. doi:10. 13271/j.mpb.014.003462.
    [8] YANG S J, YU W T, YUAN Y X, et al. Screening of SSR markers for purity identification of pepper variety ‘Yujiao No. 101’[J]. Mol Plant Breed, 2019, 17(22):7433-7437. doi:10.13271/j.mpb.017.007433. 杨双娟, 于文涛, 原玉香, 等. 辣椒品种‘豫椒101’纯度鉴定的SSR标记筛选[J]. 分子植物育种, 2019, 17(22):7433-7437. doi:10. 13271/j.mpb.017.007433.
    [9] LIU Z J, YANG Y, SUN J H, et al. Purity identification of Rela No. 2 pepper and genetic diversity analysis of excellent pepper inbred lines[J]. Chin J Trop Crops, 2014, 35(5):847-853. doi:10.3969/j.issn.1000-2561.2014.05.005. 刘子记, 杨衍, 孙继华, 等. 热辣2号辣椒纯度鉴定及优良自交系遗传多样性分析[J]. 热带作物学报, 2014, 35(5):847-853. doi:10. 3969/j.issn.1000-2561.2014.05.005.
    [10] JIA H, WEI X C, YAO Q J, et al. Genetic diversity analysis of Capsicum genus germplasm resources using SSR markers[J]. Mol Plant Breed, 2017, 15(1):353-363. doi:10.13271/j.mpb.015.000353. 贾豪, 魏小春, 姚秋菊, 等. 辣椒SSR标记种质资源遗传多样性的分析[J]. 分子植物育种, 2017, 15(1):353-363. doi:10.13271/j.mpb. 015.000353.
    [11] GUAN J J, YU Z H, YANG X H, et al. Study on the application of SSR markers in pepper (Capsicum annuum L.) DUS testing[J]. J Plant Genet Resour, 2019, 20(2):396-405. doi:10.13430/j.cnki.jpgr.20180808001. 管俊娇, 余志慧, 杨晓洪, 等. SSR标记在辣椒DUS测试中的应用研究[J]. 植物遗传资源学报, 2019, 20(2):396-405. doi:10.13430/j. cnki.jpgr.20180808001.
    [12] LI Y, ZHAO H X, WANG Y, et al. Screening and application of SSR markers' polymorphism of capsicum whole genome[J]. Chin Agric Sci Bull, 2018, 34(17):56-61. doi:10.11924/j.issn.1000-6850.casb17090062. 李艳, 赵红星, 王勇, 等. 辣椒全基因组SSR标记多态性的筛选及应用[J]. 中国农学通报, 2018, 34(17):56-61. doi:10.11924/j.issn. 1000-6850.casb17090062.
    [13] CHEN Z H, SHAN R Y, YOU X M, et al. Constructing fingerprints and analyzing genetic diversity of 43 tea cultivars in Fujian Province[J]. J Trop Subtrop Bot, 2017, 25(6):579-586. doi:10.11926/jtsb.3743. 陈志辉, 单睿阳, 游小妹, 等. 43个福建省茶树品种指纹图谱构建及遗传多样性分析[J]. 热带亚热带植物学报, 2017, 25(6):579-586. doi:10.11926/jtsb.3743.
    [14] LIANG Y S, YAN C, QIN X J, et al. Construction of three half-sib SSR linkage maps derived from overwintering cultivated rice and segre-gation distortion loci mapping[J]. Genome, 2020, 63(4):239-251. doi:10.1139/gen-2019-0160.
    [15] SHEN Z L, LI M Q, QIU T, et al. Analysis of main phenotypic traits and SSR marker diversity of F1 generation of rhododendron[J]. Jiangsu Agric Sci, 2019, 47(18):177-181. 沈梓力, 李美芹, 邱甜, 等. 杜鹃花F1代群体主要表型性状及SSR标记的多样性分析[J]. 江苏农业科学, 2019, 47(18):177-181.
    [16] ZHANG Y J, GOU Z W, WANG X R, et al. An analysis of genetic diversity and linked agronomic traits of Heshangtou wheat in northwest China[J]. Acta Pratac Sin, 2019, 28(2):142-155. doi:10.11686/cyxb 2018124. 张彦军, 苟作旺, 王兴荣, 等. 西北地区和尚头小麦遗传多样性及农艺性状的关联分析[J]. 草业学报, 2019, 28(2):142-155. doi:10. 11686/cyxb2018124.
    [17] GU J, LI L, ZONG X X, et al. Association analysis between morpho-logical traits of pea and its polymorphic SSR markers[J]. J Plant Genet Resour, 2011, 12(6):833-839. doi:10.13430/j.cnki.jpgr.2011.06.025. 顾竟, 李玲, 宗绪晓, 等. 豌豆种质表型性状SSR标记关联分析[J]. 植物遗传资源学报, 2011, 12(6):833-839. doi:10.13430/j.cnki.jpgr. 2011.06.025.
    [18] XUE D, XIAO L, AI X, et al. Genetic diversity of Miscanthus flori-dulus revealed by morphological characters and SSR markers[J]. Acta Pratac Sin, 2012, 21(5):96-106. 薛德, 肖亮, 艾辛, 等. 五节芒表型性状和SSR标记遗传多样性分析[J]. 草业学报, 2012, 21(5):96-106.
    [19] XU F R, DONG C, YANG W Y, et al. Genetic similarity based on SSR markers and phenotypies traits of major improved rice (Oryza sativa L.) varieties in Yunnan province[J]. J Plant Genet Resour, 2011, 12(5):700-708. doi:10.13430/j.cnki.jpgr.2011.05.012. 徐福荣, 董超, 杨文毅, 等. 基于表型性状和SSR分子标记的云南省水稻主要育成品种(系)的遗传相似性分析[J]. 植物遗传资源学报, 2011, 12(5):700-708. doi:10.13430/j.cnki.jpgr.2011.05.012.
    [20] FU H F, LYU X H, CEHN J Y, et al. Genetic diversity analysis of capsicum germplasm based on phenotypic traits with SSR markers[J]. J Nucl Agric Sci, 2018, 32(7):1309-1319. doi:10.11869/j.issn.100-8551.2018.07.1309. 傅鸿妃, 吕晓菡, 陈建瑛, 等. 辣椒种质表型性状与SSR分子标记的遗传多样性分析[J]. 核农学报, 2018, 32(7):1309-1319. doi:10. 11869/j.issn.100-8551.2018.07.1309.
    [21] LI X X, ZAHNG B X. Descriptors and Data Standard for Capsicum[M]. Beijing:China Agricultural Press, 2006:1-98. 李锡香, 张宝玺. 辣椒种质资源描述规范和数据标准[M]. 北京:中国农业出版社, 2006:1-98.
    [22] SUN M. Genetic diversity in three colonizing orchids with contrasting mating systems[J]. Amer J Bot, 1997, 84(2):224-232. doi:10.2307/2446084.
    [23] ZHANG J B, YAN J J, BAI S Q, et al. Genetic diversity of native Erianthus arundinaceus germplasm detected by SRAP markers[J]. J Agric Biotechnol, 2013, 21(10):1193-1202. doi:10.3969/j.issn.1674-7968.2013.10.008. 张建波, 鄢家俊, 白史且, 等. 斑茅野生种质资源SRAP遗传多样性研究[J]. 农业生物技术学报, 2013, 21(10):1193-1202. doi:10. 3969/j.issn.1674-7968.2013.10.008.
    [24] LEE J M, NAHM S H, KIM Y M, et al. Characterization and molecular genetic mapping of microsatellite loci in pepper[J]. Theor Appl Genet, 2004, 108(4):619-627. doi:10.1007/s00122-003-1467-x.
    [25] INAMIYAMA Y, TSURO M, HIRAI M. An SSR-based linkage map of Capsicum annuum[J]. Mol Breed, 2006, 18(2):157-169. doi:10.1007/s11032-006-9024-3.
    [26] PORTIS E, NAGY I, SASVÁRI Z, et al. The design of Capsicum spp. SSR assays via analysis of in silico DNA sequence, and their potential utility for genetic mapping[J]. Plant Sci, 2006, 172(3):640-648. doi:10.1016/j.plantsci.2006.11.016.
    [27] ZHENG D J, YUN T H, ZHANG Z L, et al. Study on genetic diversity and relationship for the Hainan island landraces of Cucurbita moschata[J]. J Nucl Agric Sci, 2016, 30(5):869-877. doi:10.11869/j.issn.100-8551.2016.05.0869. 郑道君, 云天海, 张治礼, 等. 中国南瓜海南农家品种资源遗传多样性和亲缘关系研究[J]. 核农学报, 2016, 30(5):869-877. doi:10. 11869/j.issn.100-8551.2016.05.0869.
    [28] XU H, LIU M G, DONG S J, et al. Diversity and geographical varia-tions of germplasm resources of Armeniaca mandshurica[J]. Chin J Plant Ecol, 2019, 43(7):585-600. doi:10.17521/cjpe.2019.0060. 徐豪, 刘明国, 董胜君, 等. 东北杏种质资源多样性及其地理变化[J]. 植物生态学报, 2019, 43(7):585-600. doi:10.17521/cjpe.2019.0060.
    [29] ZHAN Y F, YANG H, TU X M, et al. Genetic diversity of hot pepper germplasm resources and its cluster analysis[J]. Guizhou Agric Sci, 2010, 38(11):12-15. doi:10.3969/j.issn.1001-3601.2010.11.004. 詹永发, 杨红, 涂祥敏, 等. 辣椒品种资源的遗传多样性和聚类分析[J]. 贵州农业科学, 2010, 38(11):12-15. doi:10.3969/j.issn.1001-3601.2010.11.004.
    [30] HE W, ZHANG H, WANG Y. Analysis of morphology and SRAP of pepper germplasm resources[J]. Heilongjiang Agric Sci, 2019(5):7-12. doi:10.11942/j.issn1002-2767.2019.05.007. 赫卫, 张慧, 王莹. 辣椒种质资源的形态学和SRAP分析[J]. 黑龙江农业科学, 2019(5):7-12. doi:10.11942/j.issn1002-2767.2019.05. 007.
    [31] LI N, YANG T, PATIGULI, et al. Genetic diversity analysis of Xinjiang Dried-Orientation type pepper by SRAP and SCoT[J]. Mol Plant Breed, 2017, 15(8):3331-3340. doi:10.13271/j.mpb.015.003331. 李宁, 杨涛, 帕提古丽, 等. 适于新疆制干类型辣椒种质资源遗传多样性的SRAP和SCoT分析[J]. 分子植物育种, 2017, 15(8):3331-3340. doi:10.13271/j.mpb.015.003331.
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张强强,梁赛,王艳,贾利,方凌,张其安,江海坤,隋益虎,董言香.基于表型性状和SSR标记的57份辣椒种质遗传多样性分析[J].热带亚热带植物学报,2020,28(4):356~366

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  • 收稿日期:2019-12-17
  • 最后修改日期:2020-04-13
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