琴叶风吹楠资源遗传多样性的AFLP分析

Genetic Diversity of Horsfieldia pandurifolia Based on AFLP Markers

摘要:

为研究濒危植物琴叶风吹楠(Horsfieldia pandurifolia)的遗传多样性，利用AFLP分子标记技术，对采自云南省西双版纳州、临沧市的8个居群共56份琴叶风吹楠样品进行了分析。结果表明，琴叶风吹楠在物种水平的多态性较高，多态性率为75.16%；在居群水平，其平均多态性率为36.20%；AMOVA分析结果显示，琴叶风吹楠的遗传变异主要存在于居群内（75.45%），而居群间的变异仅为24.55%；mantel检验结果显示，地理距离和遗传距离存在不显著的正相关(r=0.119 7，P=0.321 0)；基于遗传相似性系数，对8个居群进行了UPGMA聚类分析，以遗传相似性系数0.951为界，8个居群聚为3组。基于以上结果，提出了保护建议，为琴叶风吹楠的保护和开发利用提供了理论依据。

关键词:琴叶风吹楠;种质资源;AFLP;遗传多样性

Abstract:

In order to study the genetic diversity of Horsfieldia pandurifolia from Xishuangbanna and Lincang of Yunnan province, genetic diversities of 56 samples from 8 populations were tested by AFLP markers. The results showed that a high genetic diversity existed in H. pandurifolia, with the percentage of polymorphic sites were 75.16% at species level, and 36.20% at population level. Moreover, a high level genetic variation (75.45%) within the population was detected through AMOVA analysis, but only existed 24.55% between populations. Analysis of correlation between geographical distance and genetic distance showed that there was positive correlation (r=0.1197，p=0.3210) among the populations，but it was not significant. Based on the UPGMA clustering analysis of the coefficient of genetic similarity, the 8 populations were divided into 3 groups at a similarity coefficient threshold of 0.951. On the basis of these results, the strategy was proposed for the conservation germplasm and laid a foundation for the development and sustainable development of H. pandurifolia.

Key words:Horsfieldia pandurifolia;Germplasm resources; AFLP; Genetic Diversity