云南48种木兰科野生植物资源遗传多样性研究
作者:
作者单位:

1.西南林业大学林学院;2.中南林业科技大学生命科学与技术学院;3.湖南农业大学

中图分类号:

S718.46

基金项目:

湖南省重点研发计划项目(2016NK2143);长沙市科技局项目“基于ISSR分子标记技术对木兰科植物育种群体评价与筛选”


Study on Genetic Diversity of 48 Wild Species of Magnoliaceae
Author:
Affiliation:

1.SouthSWestSforestSuniversity,forestScollege;2.College of Life Science and Technology,Central South University of Forestry Technology;3.Hunan Agricultural University

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [37]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    云南省是木兰科植物的避难所和发源地,原始种和进化种相间分布,对研究木兰科植物系统进化与分类具有重要意义。本研究利用ISSR分子标记技术对云南省48种木兰科野生植物资源开展研究。结果表明,10条引物共扩增出条带151条,其中多态性条带为151条,多态性条带百分比为100%。总的观测等位基因数(Na)为2.0000,有效等位基因数(Ne)为1.5645,Nei"s基因多样性指数(H)为0.3379,Shannon"s信息指数(I)为0.5101。总的基因多样性指数(Ht)为0.3680,属间基因多样性(Dst)为0.2519,占68.4%,基因分化系数(Gst)为0.6840,基因流(Nm)为0.2310。UPGMA聚类结果将48种木兰科植物划分为7个类群,聚类结果并非按照属的划分方式聚在一起,而是不同属植物相间分布,长喙厚朴、素黄含笑和球花含笑可能为云南省木兰科植物中的原始种。云南省48种木兰科野生植物总体具有较高的遗传多样性,但属间遗传变异较高,基因流较小,存在遗传漂变的风险,并且聚类结果与刘玉壶分类系统存在分歧,研究结果从分子水平上为木兰科植物间的起源、进化与分类提供了重要依据。

    Abstract:

    Yunnan province is the refuge and birthplace of magnolias, the primitive and evolutionary species of Magnoliaceae are distributed alternately in there. It is very important to study the phylogenetic evolution and classification of Magnoliaceae.In this study, ISSR molecular markers were used to study 48 species of Magnoliaceae wild plant resources in Yunnan province.The results showed that a total of 151 bands and 151 polymorphic bands were amplified from the 10 primers, and the percentage of polymorphic bands was 100%. The total number of observational alleles (Na), effective alleles (Ne), Nei"s genetic diversity index (H) and Shannon"s information index (I) of the 48 Magnoliaceae plants were 2.0000, 1.5645, 0.3379 and 0.5101, respectively. The total genetic diversity index (Ht) was 0.3680 and the intergeneric genetic diversity index (Dst) was 0.2519. The intergeneric genetic diversity accounted for 68.4% of the total genetic diversity index. The coefficient of gene differentiation (Gst) was 0.6840, and the gene flow (Nm) was 0.2310. 48 species of Magnoliaceae plants were divided into 7 groups by the UPGMA clustering, and the clustering results were not consistent with the classification of genera, but distributed in different genera.and Magnolia rostrata, Michelia flaviflora and Michelia sphaerantha may be the ancestors of Magnoliaceae in Yunnan province.The 48 species of Magnoliaceae wild plants in Yunnan province have high genetic diversity in general, but they have high inter-genus genetic variation and small gene flow, which may lead to the risk of genetic drift. Moreover, the clustering results are different from the classification system of Liu Yuhu.The results provide an important basis for the origin, evolution and classification of Magnoliaceae at the molecular level.

    参考文献
    [1] LIU K W, YANG X H. Studies on classification and geographical distribution of Magnoliaceae species in Hunan[J]. J Wuhan Bot Res, 2001, 19(2):121-127. 刘克旺, 杨旭红. 湖南木兰科植物分类和地理分布的研究[J]. 武汉植物学研究, 2001, 19(2):121-127.
    [2] LIN Q, DUAN L D, YUAN Q. Taxonomic notes on the genus Kmeria (Pierre) Dandy (Magnoliaceae)[J]. J Wuhan Bot Res, 2005, 23(3):236-238. doi:10.3969/j.issn.2095-0837.2005.03.008. 林祁, 段林东, 袁琼. 单性木兰属(木兰科)植物的分类学订正[J]. 武汉植物学研究, 2005, 23(3):236-238. doi:10.3969/j.issn.2095-0837.2005.03.008.
    [3] LIN Q, DUAN L D, YUAN Q, et al. Taxonomic revision of the genus Parakmeria Hu & Cheng (Magnoliaceae)[J]. Bull Bot Res, 2006, 26(5):527-531,538. doi:10.7525/j.issn.1673-5102.2006.05.005. 林祁, 段林东, 袁琼, 等. 拟单性木兰属(木兰科)植物的分类学修订[J]. 植物研究, 2006, 26(5):527-531,538. doi:10.7525/j.issn.1673-5102.2006.05.005.
    [4] SUN W B, ZHOU J. A new proposal on generic division of the Chinese Magnoliaceae[J]. Acta Bot Yunnan, 2004, 26(2):139-147. doi:10.3969/j.issn.2095-0845.2004.02.002. 孙卫邦, 周俊. 中国木兰科植物分属的新建议[J]. 云南植物研究, 2004, 26(2):139-147. doi:10.3969/j.issn.2095-0845.2004.02.002.
    [5] BARANOVA M. Systematic anatomy of the leaf epidermis in the Magnoliaceae and some related families[J]. Taxon, 1972, 21(4):447-469. doi:10.2307/1219106.
    [6] ZHOU S B, ZOU G S, ZHANG X W, et al. Comparative anatomy of leaves in Magnoliaceae and ITS systematic significance[J]. J Nanjing For Univ, 1996, 20(3):31-34. doi:10.3969/j.jssn.1000-2006.1996.03.008. 周守标, 邹高顺, 张学武, 等. 木兰科叶结构的比较解剖及其系统学意义[J]. 南京林业大学学报, 1996, 20(3):31-34. doi:10.3969/j. jssn.1000-2006.1996.03.008.
    [7] HUANG L F. RAPD and ISSR analysis of 20 species in 6 genera from Magnoliaceae[D]. Fuzhou:Fujian Normal University, 2007:1-72. 黄丽峰. 木兰科6属20种植物的RAPD和ISSR分析[D]. 福州:福建师范大学, 2007:1-72.
    [8] LI J. Analysis of genetic diversity of 21 kinds of Magnoliaceae ever-green plants[J]. Zhengzhou:Henan Agricultural University, 2013:1-43. 李剑. 21种木兰科常绿植物的遗传多样性分析[D]. 郑州:河南农业大学, 2013:1-43.
    [9] KIM S, PARK C W, KIM Y D, et al. Phylogenetic relationships in family Magnoliaceae inferred from ndhF sequences[J]. Amer J Bot, 2001, 88(4):717-728. doi:10.2307/2657073.
    [10] WANG Y L, LI Y, ZHANG S Z, et al. The utility of matK gene in the phylogenetic analysis of the genus Magnolia[J]. Acta Phytotaxon Sin, 2006, 44(2):135-147. doi:10.1360/aps040013. 王亚玲, 李勇, 张寿洲, 等. 用matK序列分析探讨木兰属植物的系统发育关系[J]. 植物分类学报, 2006, 44(2):135-147. doi:10.1360/aps040013.
    [11] WANG Y L, ZHANG S Z, CUI T C. The utility of trnL intron and trnL-trnF IGS in phylogenetic analysis of Magnoliaceae[J]. Acta Bot Boreali-Occid Sin, 2003, 23(2):247-252. doi:10.3321/j.issn:1000-4025.2003.02.010. 王亚玲, 张寿洲, 崔铁成. trnL内含子及trnL-trnF间隔区序列在木兰科系统发育研究中的应用[J]. 西北植物学报, 2003, 23(2):247-252. doi:10.3321/j.issn:1000-4025.2003.02.010.
    [12] ZHAO J H, JIA Q, JIN H T, et al. Evolution analysis of the chloroplast genes atpB and rbcL of Magnolia[J]. J Changchun Norm Univ (Nat Sci), 2014, 33(6):71-74. 赵晶华, 贾倩, 金洪涛, 等. 木兰叶绿体atpB和rbcL基因的系统进化分析[J]. 长春师范大学学报(自然科学版), 2014, 33(6):71-74.
    [13] SHEN Y M, CHEN K, GU C H, et al. Comparative and phylogenetic analyses of 26 Magnoliaceae species based on complete chloroplast genome sequences[J]. Can J For Res, 2018, 48(12):1456-1469. doi:10.1139/cjfr-2018-0296.
    [14] ZIETKIEWICZ E, RAFALSKI A, LABUDA D. Genome finger-printing by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2):176-183. doi:10.1006/geno.1994.1151.
    [15] JIANG X L, ZHANG J H, LI Y X, et al. Genetic diversity analysis of Photinia based on ISSR molecular markers[J]. Zhejiang Agric Sci, 2019, 60(4):636-639. doi:10.16178/j.issn.0528-9017.20190434. 蒋笑丽, 章建红, 李玉祥, 等. 基于ISSR分子标记的石楠属植物遗传多样性分析[J]. 浙江农业科学, 2019, 60(4):636-639. doi:10.16178/j.issn.0528-9017.20190434.
    [16] LI J, DONG L J, LIN J Y, et al. The ISSR analysis of Phoebe Nees germplasm resources[J]. Mol Plant Breed, 2018, 16(19):6428-6435. doi:10.13271/j.mpb.016.006428. 李娟, 董利军, 林建勇, 等. 楠木树种种质资源的ISSR分析[J]. 分子植物育种, 2018, 16(19):6428-6435. doi:10.13271/j.mpb.016.006428.
    [17] YOU X L, LUO Y L, YIN L J, et al. Development of ISSR markers and analysis of genetic diversity of Asimina triloba[J]. For Sci Technol, 2018(11):3-6. doi:10.13456/j.cnki.lykt.2018.09.18.0001. 有祥亮, 罗玉兰, 尹丽娟, 等. 北美泡泡树ISSR标记开发及遗传多样性分析[J]. 林业科技通讯, 2018(11):3-6. doi:10.13456/j.cnki. lykt.2018.09.18.0001.
    [18] LIU J, LIAO K, ZHAO S R, et al. The core collection construction of Xinjiang wild apricot based on ISSR molecular markers[J]. Sci Agric Sin, 2015, 48(10):2017-2028. doi:10.3864/j.issn.0578-1752.2015.10.014. 刘娟, 廖康, 赵世荣, 等. 利用ISSR分子标记构建新疆野杏核心种质资源[J]. 中国农业科学, 2015, 48(10):2017-2028. doi:10.3864/j. issn.0578-1752.2015.10.014.
    [19] JU X T, PAN A Q, JIANG F J, et al. Genetic diversity of tulip germ-plasm resource revealed by ISSR markers[J]. Gen Appl Biol, 2018, 38(8):3667-3674. doi:10.13417/j.gab.038.003667. 巨秀婷, 潘阿青, 蒋福娟, 等. 郁金香种质资源遗传多样性的ISSR分析[J]. 基因组学与应用生物学, 2018, 38(8):3667-3674. doi:10.13417/j.gab.038.003667.
    [20] PENG J Q, XU Z G, CAO F X, et al. Study on genetic diversity of varieties of Hydrangea macrophylla and Hydrangea paniculata by ISSR markers[J]. Jiangsu Agric Sci, 2017, 45(9):18-22. doi:10.15889/j.issn.1002-1302.2017.09.005. 彭继庆, 徐志高, 曹福祥, 等. 绣球和圆锥绣球品种遗传多样性ISSR研究[J]. 江苏农业科学, 2017, 45(9):18-22. doi:10.15889/j. issn.1002-1302.2017.09.005.
    [21] PENG J Q, CAO F X, CAO J W, et al. Genetic diversity and finger-print construction of varieties of Hydrangea macrophylla by ISSR markers[J]. J CS Univ For Technol, 2016, 36(12):115-120. doi:10.14067/j.cnki.1673-923x.2016.12.020. 彭继庆, 曹福祥, 曹基武, 等. 绣球花品种遗传多样性的ISSR分析及指纹图谱构建[J]. 中南林业科技大学学报, 2016, 36(12):115-120. doi:10.14067/j.cnki.1673-923x.2016.12.020.
    [22] PENG J Q, CAO F X, XU R X. Establishment and optimization of ISSR-PCR reaction system of Mytilaria laosensis[J]. Chin Agric Sci Bull, 2012, 28(16):53-59. doi:10.3969/j.issn.1000-6850.2012.16.010. 彭继庆, 曹福祥, 许若娴. 壳菜果ISSR-PCR反应体系的建立与优化[J]. 中国农学通报, 2012, 28(16):53-59. doi:10.3969/j.issn.1000-6850.2012.16.010.
    [23] LIU Y H. Flora Repubulicae Popularis Sinicae, Tomus 30(1)[M]. Beijing:Science Press, 1996:151-191. 刘玉壶. 中国植物志, 第30卷第1分册[M]. 北京:科学出版社, 1996:151-191.
    [24] DONG Y C. Detection of biological diversity and crop genetic diversity[J]. Plant Var Resour, 1995(3):1-5. doi:10.19462/j.cnki.1671-895x. 1995.03.001. 董玉琛. 生物多样性及作物遗传多样性检测[J]. 作物品种资源, 1995(3):1-5. doi:10.19462/j.cnki.1671-895x.1995.03.001.
    [25] YU H H, YANG Z L, SUN B, et al. Genetic diversity and relationship of endangered plant Magnolia officinalis (Magnoliaceae) assessed with ISSR polymorphisms[J]. Biochem Syst Ecol, 2011, 39(2):71-78. doi:10.1016/j.bse.2010.12.003.
    [26] HAMRICK J L, GODT M J W, SHERMAN-BROYLES S L. Factors influencing levels of genetic diversity in woody plant species[J]. New For, 1992, 6(1):95-124. doi:10.1007/BF00120641.
    [27] SUN F, YANG M S, ZHANG J, et al. ISSR analysis of genetic diversity of Robinia pseudoacacia populations[J]. J Plant Genet Resour, 2009, 10(1):91-96. 孙芳, 杨敏生, 张军, 等. 刺槐不同居群遗传多样性的ISSR分析[J]. 植物遗传资源学报, 2009, 10(1):91-96.
    [28] YANG M S, HERTEL H, SCHNECK V. Allozyme variability of provenance populations of Robinia pseudoacacia from Middle Europe[J]. Acta Genet Sin, 2004, 31(12):1439-1447. 杨敏生, HERTEL H, SCHNECK V. 欧洲中部刺槐种源群体等位酶变异研究[J]. 遗传学报, 2004, 31(12):1439-1447.
    [29] NYBOM H, BARTISH I V. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants[J]. Persp Plant Ecol Evol Syst, 2000, 3(2):93-114. doi:10.1078/1433-8319-00006.
    [30] WRIGHT S. Evolution in Mendelian populations[J]. Bull Math Biol, 1990, 52(1/2):241-295. doi:10.1016/S0092-8240(05)80011-4.
    [31] NYBOM H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J]. Mol Ecol, 2004, 13(5):1143-1155. doi:10.1111/j.1365-294X.2004.02141.x.
    [32] QIU Y L, CHASE M W, PARKS C R. A chloroplast DNA phylogenetic study of the eastern Asia-eastern North America disjunct section Rytidospermum of Magnolia (Magnoliaceae)[J]. Amer J Bot, 1995, 82(12):1582-1588. doi:10.1002/j.1537-2197.1995.tb13861.x.
    [33] MA X, CHEN S Y, ZHANG X Q, et al. Genetic diversity of gliadin in worldwide germplasm collections of Elymus sibiricus[J]. Acta Pratacul Sin, 2009, 18(3):59-66. doi:10.3321/j.issn:1004-5759.2009.03.009. 马啸, 陈仕勇, 张新全, 等. 老芒麦种质的醇溶蛋白遗传多样性研究[J]. 草业学报, 2009, 18(3):59-66. doi:10.3321/j.issn:1004-5759.2009.03.009.
    [34] HINSINGER D D, STRIJK J S. The chloroplast genome sequence of Michelia alba (Magnoliaceae), an ornamental tree species[J]. Mito-chond DNA Part B, 2017, 2(1):9-10. doi:10.1080/23802359.2016.1275850.
    [35] NOOTEBOOM H P. Notes on Magnoliaceae I[J]. Blumea, 1985, 31(1):65-121.
    [36] WANG M C, MIN C L. A new species of Magnolia from Shanxi[J]. Acta Bot Boreali-Occid Sin, 1992, 12(1):85-86. 王明昌, 闵成林. 陕西木兰属一新种[J]. 西北植物学报, 1992, 12(1):85-86.
    [37] DING B Z, ZHAO T B, WANG S Y, et al. New species and variations of Cymbidium from Henan[J]. J Henan Agric Coll, 1983(4):6-11. doi:10.16445/j.cnki.1000-2340.1983.04.002. 丁宝章, 赵天榜, 王遂义, 等. 河南木兰属新种和新变种[J]. 河南农学院学报, 1983(4):6-11. doi:10.16445/j.cnki.1000-2340.1983.04.002.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

王猛,李娇婕,薛超,曹基武,曹福祥,彭继庆,吴毅.云南48种木兰科野生植物资源遗传多样性研究[J].热带亚热带植物学报,2020,28(3):277~284

复制
分享
文章指标
  • 点击次数:795
  • 下载次数: 632
  • HTML阅读次数: 693
  • 引用次数: 0
历史
  • 收稿日期:2019-09-30
  • 最后修改日期:2020-02-28
  • 录用日期:2020-03-05
  • 在线发布日期: 2020-06-29
文章二维码