6种拟南芥突变体热敏感性差异的比较分析

Comparison of Thermo-sensitivity among Six Mutants of Arabidopsis thaliana

摘要:

为了解拟南芥(Arabidopsis thaliana)热敏感突变体的热敏感性，对6种常用的拟南芥热敏感突变体，hot1、apx2、fes1a、hsfa7a、hop1-2-3及hsp70-15进行了比较分析。结果表明，6种突变体的热敏感性均高于野生型，但他们之间的热敏感性有显著差异，45℃极度高温下90 min，hot1的白化死亡率最高，处理105 min后，fes1a也出现高比率的白化死亡，处理135 min后，apx2、hsfa7a和hop1-2-3表现出几乎相同的损伤现象，热损伤均比hsp70-15严重。因此, 6种突变体的热敏感性依次为hot1>fes1a>apx2、hsfa7a和hop1-2-3> hsp70-15。

Abstract:

In order to understand the thermo-sensitivity of heat sensitive mutants of Arabidopsis thaliana, the thermo-sensitivities were compared among six mutants, including hsp70-15, apx2, hop1-2-3, hsfa7a, fes1a and hot1. The results showed that the thermo-sensitivities of six mutants were higher than that of WT, but there were significant difference among them. The 8-day-old seedlings were adapted at 38℃ for 2 h at first, then they were under heat stress at 45℃. Seedlings of mutant hot1 showed albinism with high mortality under 45℃ for 90 min. Seedlings of mutant fes1a also suffered high death rate under 45℃ for 105 min. Further, seedlings of mutants apx2, hsfa7a and hop1-2-3 showed almost identical injury phenomenon under 45℃ for 135 min, their heat injury were more severe than that of mutant hsp70-15. Therefore, the thermo-sensitivities of six mutants were in the order of hot1>fes1a>apx2, hsfa7a and hop1-2-3>hsp70-15.