宁前胡内生真菌Fusarium tricinctum固体发酵代谢产物研究

Metabolites of Fusarium tricinctum, An Endophytic Fungus Isolated from Ningguo Peucedanum praeruptorum on Solid Fermentation

摘要:

为了解宁前胡内生真菌Fusarium tricinctum固体发酵代谢产物的化学成分，采用硅胶色谱、中压以及制备液相等方法对其进行分离鉴定和抗肿瘤活性研究。结果表明，从其中分离得到14个化合物，结构鉴定分别为fungerin（1）、fusagerins F（2）、6-[（3R）-3-hydroxybutan-2-yl]-5-（hydroxymethyl）-4-methoxy-2H-pyran-2-one（3）、6-[（3S）-3-hydroxybutan-2-yl]-5-（hydroxymethyl）-4-methoxy-2H-pyran-2-one（4）、6α-chlamydosporol（5）、6β-chlamydosporol（6）、（3R，4R）-（-）-4-hydroxymellein（7）、（3R）-（-）-8-hydroxymellein（8）、enniatin B（9）、enniatin B1（10）、enniatin A1（11）、enniatin A（12）、enniatin B3（13）和enniatin B2（14）。所有化合物均为首次从宁前胡内生真菌中分离得到。体外抗肿瘤活性试验表明化合物均无显著的细胞毒活性。

Abstract:

To investigate the chemical metabolites from Fusarium tricinctum, an endophytic fungus isolated from Ningguo Peucedanum praeruptorum, 14 compounds were isolated from solid fermentation and purified by silica gel column chromatography, MPLC, and preparative liquid chromatography. Based on data of MS and NMR, their structures were identified as fungerin (1), fusagerins F (2), 6-[(3R)-3-hydroxybutan-2-yl]-5-(hydroxymethyl)-4-methoxy-2H-pyran-2-one (3), 6-[(3S)-3-hydroxybutan-2-yl]-5-(hydroxymethyl)-4-methoxy-2H-pyran-2-one (4), 6α-chlamydosporol (5), 6β-chlamydosporol (6), (3R,4R)-(-)-4-hydroxymellein (7), (3R)-(-)-8-hydroxymellein (8), enniatin B (9), enniatin B1 (10), enniatin A1 (11), enniatin A (12), enniatin B3 (13), enniatin B2 (14). All these compounds are isolated from this endophytic fungus for the first time. The cytotoxicity assay of all compounds did not show anti-tumor activities.