不同成熟度树葡萄叶片中类黄酮合成转录组基因分析

Transcriptome Analysis of Flavonoid Synthesis Related Genes in Different Maturity Leaves of Myrciaria cauliflora

摘要:

为了解树葡萄（Myrciaria cauliflora）类黄酮合成相关酶差异表达基因信息，对其幼叶和成熟叶进行全转录组测序并比较分析。结果表明，从树葡萄幼叶和成熟叶中获得59 321条单基因簇（Unigenes），在8大数据库共注释到32 912条Unigenes信息，其中类黄酮合成代谢相关酶基因77个，在成熟叶片中显著下调表达的基因6个，包括2个CHI、1个CHS、1个F3H、1个2-羟基异黄酮脱水酶基因和1个ANS。5个差异表达基因经qRT-PCR验证的结果与转录组测序结果相符合。因此，树葡萄叶片中含有大量不同种类黄酮合成代谢相关酶家族基因，成熟叶片中类黄酮含量显著减少是由于少量相关基因显著下调。

Abstract:

The flavonoid content in immature leaves was significantly higher than that in mature leaves of Myrciaria cauliflora. In order to understand the information of differentially expressed genes related flavonoid synthesis in M. cauliflora, the transcriptome of 2 kinds of leaves were sequenced by Illumina HiSeq method. The results showed that there were 59 321 Unigenes obtained from young and mature leaves, among which 32 912 unigenes were annotated by 8 databases. There were 77 Unigenes related flavonoid anabolism, and 6 genes were down-regulated significantly in mature leaves, including two CHIs, one CHS, one F3H, one 2-hydroxyiso-flavanone dehydratase gene and one ANS. The RT-PCR verified results of five differentially expressed genes were agreed with the transcriptome sequencing. So, it was suggested that there were a large number of family genes related flavonoid anabolism in the leaves of M. cauliflora, but the decrease of flavonoid content in mature leaves was due to several down-regulated genes.