油莎豆快速繁殖体系的研究

Studies on Rapid Propagation System of Yellow Nutsedge (Cyperus esculentus L.) in vitro

摘要:

为建立油莎豆（Cyperus esculentus）的快繁体系，以茎尖为外植体，MS为基本培养基，采用正交设计法研究了6-BA、KT和NAA组合对丛生芽增殖和生根的影响。结果表明，3种生长调节剂对油莎豆丛生芽增殖的影响为6-BA>NAA>KT，最佳生长调节剂组合为1.0 mg L^-1 6-BA+0.2 mg L^-1 KT，培养4周的增殖系数为7.58。MS培养基为最优生根培养基，生根率可达88.10%，平均生根数为2.04条。这为高效繁育油莎豆优质种苗和种质资源优化奠定基础。

Abstract:

In order to construct rapid propagation system of yellow nutsedge (Cyperus esculentus), the effect of growth regulator combinations, including 6-BA, KT and NAA, was studied by using orthogonal design method with shoot tip as explants on basic medium of MS. The results showed that the effects of growth regulators on proliferation of multiole shoots was in order of 6-BA > NAA > KT. The optimal growth regulator combination was 1.0 mg L^-1 6-BA+0.2 mg L^-1 KT with proliferation coefficient of 7.58 after cultured for 4 weeks. MS medium was optimal rooting medium with rooting rate of 88.10%, and an average roots was 2.04. So, these would lay a foundation for efficient breeding of high quality seedlings and optimization of germplasm resource of yellow nutsedge.