黄藤染色体核型及基因组大小分析

Karyotype and Genome Size Analyses of Daemonorops jenkinsiana

摘要:

为探究黄藤（Daemonorops jenkinsiana）染色体核型和基因组的大小，采用体细胞染色体常规制片法与显微摄影技术相结合的方法，对黄藤染色体进行了核型分析，同时以番茄（Lycopersicon esculentum）为内标，应用流式细胞术对黄藤叶片基因组大小、DNA含量和DNA倍性进行了测定。结果表明，黄藤茎尖是理想的染色体制片材料；黄藤的染色体数为2n=24，核型公式为K（2n）=1M+17m+5sm+1st，核型类型为2C；核型不对称系数61.20%；黄藤的DNA含量为1.57 pg，基因组大小为1 539.53 Mb，黄藤的DNA倍性为二倍体（2n）。这是首次报道黄藤的核型和基因组大小，为深入开展黄藤属及其近缘属植物的核型和基因组比较分析提供了参考依据。

Abstract:

In order to explore the karyotype and genome size of Daemonorops jenkinsiana, the root and stem tips from its seedlings were used as experimental materials. The karyotype of D. jenkinsiana, including chromosome number and morphology were analyzed by combining the conventional method of somatic cell chromosome preparation with microphotography. Meanwhile, the DNA content, genome size and ploidy of leaves were determined by flow cytometry using Lycopersicon esculentum as an internal reference. The results showed that the stem tip of D. jenkinsiana was an ideal material for chromosome preparation, and the chromosome number was 2n=24, along with the formula of karyotype was K(2n)=1M+17m+5sm+1st, and the karyotype was "2C". The karyotype asymmetry index of D. jenkinsiana was 61.20%. The DNA content of D. jenkinsiana was about 1.57 pg with the genome size of 1 535.53 Mb, and the DNA ploidy was diploid (2n). This was reported the karyotype and genome size of D. jenkinsiana for the first time, which provided a reference for studying the karyotype and genome of other species in Daemonorops and related genus.