冰菜盐胁迫下的转录组分析

Transcriptome Analysis of Mesembryanthemum crystallinum under Salt Stress

摘要:

为了解冰菜（Mesembryanthemum crystallinum）叶片抗盐相关基因组学，利用Illumina Hi-seq TM2500高通量测序技术研究冰菜叶片在400 mmol L^-1 NaCl胁迫下转录组基因的差异表达。结果表明，从400 mmol L^-1 NaCl胁迫和对照的冰菜叶片中共获得13.01 Gb Clean data，Q30碱基均大于90.08%。共获得123个差异表达基因（DEGs），包括73个上调基因，50个下调基因，其中功能注释的基因有96个。根据Unigene库序列进行GO、COG和KEGG注释，筛选出8个与抗盐性相关差异表达基因，植物激素代谢相关基因，脱落酸8'-羟基化酶、吲哚-3-乙酰酸酰胺合成酶和茉莉酮酸酯ZIM结构域蛋白基因均下调表达，生长素响应蛋白、细胞分裂素合酶基因则上调表达，糖代谢相关基因棉子糖合成酶基因上调表达，质膜H⁺-ATPase基因上调表达，脱水蛋白基因下调表达。这为冰菜耐盐基因组学和分子生物学的研究奠定基础。

Abstract:

In order to understand the salt related genomics in leaves of Mesembryanthemum crystallinum, the expression of transcriptome genes was studied under 400 mmol L^-1 NaCl stress by using Illumina Hiseq TM2500 high-throughput sequencing technology. The results showed that there were 13.01 Gb Clean Data from the leaves treated with 400 mmol L^-1 NaCl and untreated, the Q30 base was more than 90.08%. Among them, there were 123 differential expression genes (DEGs), including 73 up-regulated genes and 50 down-regulated genes, in which 96 genes were annotated. The eight salt-resistance DEGs of were screened out based on GO, COG and KEGG annotation. The plant hormone metabolism-related genes, including abscisic acid 8'-hydroxylase gene, indole-3-acetamide synthase gene, jasmonate ZIM structure domain protein gene were down-regulated, auxin responsive protein and cytokinin synthase genes were up-regulated. The sugar metabolism-related raffinose synthase genes was up-regulated, and plasma membrane H⁺-ATPase and dehydrated protein genes were up and down-regulated, respectively. These would provide a foundation for studing on salt tolerance genomics and molecular biology of M. crystallinum.