国产绿奇楠沉香的化学成分研究

Study on Chemical Constituents of Chinese Agarwood ‘Qi-Nan’

摘要:

为了解国产绿‘奇楠’沉香的化学成分，采用色谱和波谱方法从其乙醚和乙醇提取物中分离鉴定了7个化合物，分别鉴定为顺式-7-羟基菖蒲烯（1），（5R，6R，7S，8R）-2-（苯乙基）-6，7，8-三羟基-5，6，7，8-四氢-5-[2-（2-苯乙基）色酮基-6-氧代]色酮（2），1-羟基-1，5-二苯基戊-3-酮（3），丁香树脂酚葡萄糖苷（4），（3β）-齐墩果-12-烯-3，23-二醇（5），β-谷甾醇（6）和棕榈酸-α-单甘油酯（7）。化合物1、3~5和7均为首次从沉香中分离得到，其中化合物1表现出非常甜的芳香气味。乙酰胆碱酯酶体外抑制活性测试结果表明，50 μmol L^-1的化合物1对乙酰胆碱酯酶抑制率为（49.9±1.4）%。

关键词:奇楠;沉香;化学成分;乙酰胆碱酯酶;抑制活性

Abstract:

To understand the chemical constituents of Chinese agarwood "Qi-Nan", seven compounds were isolated by various column chromatographic methods. On the basis of spectral data, they were identified as cis-7-hydroxycalamenene (1), (5R,6R,7S,8R)-2-(phenylethyl)-6,7,8-trihydroxy-5,6,7,8-tetrahydro-5-[2-(2-phenylethyl) chromonyl-6-oxy]chromone (2), 1-hydroxy-1,5-diphenylpentan-3-one (3), syringaresinol-glycoside (4), (3β)- olean-12-ene-3,23-diol (5), β-sitosterol (6) and hexadecanoic acid, 2,3-dihydroxypropyl ester (7). Compounds 1, 3-5, and 7 were isolated from agarwood for the first time, and compound 1 showed a very sweet aromatic odor. Compound 1 showed acetylcholinesterase (AChE) inhibitory activity with the inhibition rate of (49.9±1.4)% at the concentration of 50 μmol L^-1.

Key words:‘Qi-Nan’;Chinese agarwood;Chemical constituent;Acetylcholinesterase (AChE);Inhibitory activity