铁皮石斛内生真菌次生代谢产物研究

Study on Secondary Metabolites of Endophytic Fungus Phyllosticta aristolochiicola from Dendrobium officinale

摘要:

为了解铁皮石斛（Dendrobium officinale）内生真菌Phyllosticta aristolochiicola的次生代谢产物，从该真菌中分离得到15个化合物，经波谱分析分别鉴定为N-methyl-2-pyrolidinone（1）、环-（甘氨酸-L-脯氨酸）（2）、环-（D-丙氨酸-L-脯氨酸）（3）、环-（L-缬氨酸-L-脯氨酸）（4）、环-（L-亮氨酸-L-脯氨酸）（5）、cyclo-（L-Leu-D-4-hydroxyprolinyl）（6）、环-（L-苯丙氨酸-L-脯氨酸）（7）、环-（L-苯丙氨酸-L-4-羟基脯氨酸）（8）、环-（L-酪氨酸-L-脯氨酸）（9）、环-（L-苯丙氨酸-L-亮氨酸）（10）、啤酒甾醇（11）、对羟基苯乙醇（12）、对羟基苯乙酸（13）、（2S，3R）-1-（4-羟基苯基）丁烷-2，3-二醇（14）和（2R，3S）-1-苯基丁烷-2，3-二醇（15）。采用MTS法检测抗肿瘤活性表明，化合物2、10和14对HL-60、A-549、SMMC-7721、MCF-7和SW-480细胞株具有一定的抑制活性。

Abstract:

In order to understand the chemical constituents of endophytic fungus Phyllosticta aristolochiicola from Dendrobium officinale, fifteen compounds were isolated. On the basis of spectral data, their structures were identified as N-methyl-2-pyrolidinone (1), cyclo-(glycine-L-proline) (2), cyclo-(D-alanine-L-proline) (3), cyclo-(L-valine-L-proline) (4), cyclo-(L-leucine-L-proline) (5), cyclo-(L-leucine-D-4-hydroxyprolinyl) (6), cyclo-(L-phenylalanine-L-proline) (7), cyclo-(L-4-hydroxyproline-L-phenylalanine) (8), cyclo-(L-tyrosine-L-proline) (9), cyclo-(L-phenylalanine-L-leucine) (10), beer sterol (11), p-hydroxyphenylethanol (12), p-hydroxyphenylacetic acid (13), (2S,3R)-1-(4-hydroxyphenyl)butane-2,3-diol (14), and (2R,3S)-1-phenylbutane-2,3-diol (15). The MTS assay showed that compounds 2, 10 and 14 had weak inhibitory activities against five human tumor cell lines, such as HL-60, A-549, SMMC-7721, MCF-7, and SW-480.