甘蔗与斑茅BC1分子鉴定、抗黑穗病和花叶病初步评价

Molecular Identification and Resistance Evaluation to Smut and Mosaic Disease with BC1 of Saccharum×Erianthus arundinaceus

摘要:

为了解甘蔗（Saccharum）与斑茅（Erianthus arundinaceus）杂交后代作为抗病亲本的利用价值，通过特异引物PCR鉴定出78份甘蔗与斑茅杂交BC₁真实杂种；通过人工接种花叶病毒和黑穗病菌，初步评价了甘蔗和斑茅杂交BC₁的抗病表现。结果表明，甘蔗和斑茅杂交BC₁的抗花叶病具有普遍性，而黑穗病抗性则出现分离。初步筛选出BC₁无性系YCE01-48、YCE01-71、YCE01-105、YCE01-125、YCE02-184和YCE01-118可同时抗花叶病和黑穗病，有望成为甘蔗杂交利用的高抗病源亲本。

Abstract:

In order to understand the utilization value of hybrids between Saccharum and Erianthus arundinaceus as resistant parents, the resistance to smut and SCMV of some progenies BC₁ from Saccharum×E. arundinaceus were evaluated by artificial inoculation of pathogens. The results showed that most of BC₁ had the resistance to SCMV, and the resistance to smut was segregative among progenies. The BC₁ clones YCE01-48, YCE01-71, YCE01-105, YCE01-125, YCE02-184 and YCE01-118 showed co-resistance to smut and SCMV. So, they could be used as parents with high disease resistance for sugarcane breeding.