基于宏基因组的茉莉花内生细菌多样性分析

Analysis of Endophytes Diversity in Jasminum sambac (L.) Ait by Metagenomic Technology

摘要:

为了解茉莉花（Jasminum sambac）的内生菌多样性，利用16S rDNA高通量测序技术对其内生细菌组成进行测定。结果表明，茉莉花的内生细菌有38门78纲150目257科434属，其中变形菌门、放线菌门、壁厚菌门、拟杆菌门和绿弯菌门的物种丰度较高。变形菌门在6-10月的丰度均最高（70.3%~85.7%）。7月份植株的细菌Shannon指数最高（3.14）、Simpson指数最小（0.13），表明细菌群落多样性最高，且高温有利于提高植株内生细菌多样性。聚类和主成分分析表明，7和8月、9和10月的植株内生细菌群落构成相似度高，6月与其他月份的差异大。冗余分析和热图分析表明，土壤营养成分影响植株内生群落组成，以全氮、全磷和有机质含量为主要。这为挖掘利用茉莉花有益的内生菌资源奠定基础。

关键词:茉莉花;内生细菌;多样性;土壤营养成分

Abstract:

In order to understand the endophytes deversity in Jasminum sambac (L.) Ait, the endophytes component was determined based on high throughput sequencing of 16S rDNA. The results showed that the endophytes in J. sambac was very rich, belonging to 38 phyla, 78 classes, 150 orders, 257 families and 434 genera. The Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Chloroflexi had high abundance. The abundance of Proteobacteria was the highest from June to October, accounting for 70.3%-85.7%. The Shannon index and Simpson index of endophytes were the biggest (3.14) and the smallest (0.13) in July, respectively, showing the highest bacterial diversity, which suggested that high temperature was beneficial to increase endophytes diversity. According to clustering and principal component analysis, the endophytes community structure in plants had high similarity in July and August, September and October, while that in June were obviously different from those in other months. Heatmap and redundancy analysis indicated that endophytes diversity were singificantly influenced by soil nutrient ingredients, and the contents of organic matter, total phosphorus and nitrogen were major factors. Therefore, these would provide a foundation for developing benefical endophytes from J. sambac.

Key words:Jasminum sambac;Endophytes;Diversity;Soil nutrient