PEG-6000模拟干旱胁迫对黄秋葵种子萌发和幼苗生理特性的影响

Effect of Drought Stress with PEG-6000 on Seed Germination and Physiological Properties in Abelmoschus esculentus

摘要:

为了解黄秋葵（Abelmoschus esculentus）对干旱胁迫的应答机制，采用PEG-6000溶液模拟干旱胁迫，对‘纤指’和‘红秋葵’品种的种子萌发和幼苗的生理特性进行研究。结果表明，两品种的种子吸水率、发芽势、发芽率、发芽指数、活力指数、简易活力指数，幼苗下胚轴长、根长、苗鲜重和苗干重均随PEG-6000浓度的增加呈下降的趋势，旱害率则不断增加。PEG-6000浓度为20%时，两品种的种子均未能发芽。幼苗的SOD、POD和CAT活性和可溶性蛋白、可溶性糖、游离脯氨酸、丙二醛含量随PEG-6000浓度的增加而增加，且均PEG-6000浓度为15%时最高。因此，干旱胁迫会抑制黄秋葵种子的萌发，提高细胞内含物的含量，同时引发其抗氧化系统的响应。

Abstract:

To understand the response mechanism of okra (Abelmoschus esculentus) under drought stress, PEG-6000 solution was used to simulate drought stress, the seed germination and physiological traits of seedlings in ‘Qianzhi’ and ‘Red okra’ varieties were studied. The results showed that the water absorption, germination energy, germination rate, germination index, vigor index, simple vigor index of seeds, and hypocotyl length, root length, fresh weight and dry weight of seedlings in two varieties decreased with the concentration of PEG-6000 solution increased, while the drought-injury rate of okra increased. The seeds of two varieties failed to germinate with 20% PEG-6000. The activities of SOD, POD and CAT, and the contents of proline (Pro), soluble protein, soluble sugar and malonaldehyde (MDA) of okra seedlings increased with the concentration of PEG-6000 solution increased, and they reached up to maximum under 15% PEG-6000. So, it was suggested that under drought stress, seed germination of A. esculentus was inhibited, the contents of cell inclusion enhanced, and the antioxidant system was stimulated.