木豆不同品种和叶龄对叶片氨基酸形成的影响和聚类分析
作者:
基金项目:

福建省农业科学院科技创新团队建设项目(STIT2017-2-11);福建省公益类科研院所专项(2016R1025-1);福建省重大专项(2018NZ0003-2);福建省科技重大专项(2015NZ0002-3)资助


Effects of Different Germplasms and Leaf Ages on Amino Acid Formation in Pigeonpea Leaves and Cluster Analysis
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [17]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为了解木豆(Cajanus cajan)叶片的氨基酸含量的变化,对19份种质资源和1~3叶龄的木豆叶片氨基酸含量进行研究。结果表明,不同种质资源和叶龄的木豆叶片中均含有17种氨基酸,必需氨基酸以苏氨酸、异亮氨酸和赖氨酸的含量最高,分别为0.67%、0.70%和0.48%,非必需氨基酸以丝氨酸、酪氨酸和组氨酸的含量最高,分别为0.69%、0.66%和0.44%。除赖氨酸和丙氨酸外,其余15种氨基酸含量在木豆叶片中总体上随着叶龄的增加呈现上升趋势。不同种质资源的木豆叶片中总氨基酸含量差异明显。聚类分析可将19个木豆种质资源划分为低氨基酸型(I)、中氨基酸型(II)、中高氨基酸型(III)、高氨基酸型(IV)等4大类型。这为高品质木豆叶茶的加工与高氨基酸木豆品种选育提供了理论依据。

    Abstract:

    In order to understand the changes in amino acid contents of pigeonpea (Cajanus cajan) leaves, the amino acid contents from 19 pigeonpea germplasms at 1-3-leaf-old were determined. The results showed that there were 17 kinds of amino acids in leaves of all pigeonpea germplasms at different leaf ages. The contents of threonine, isoleucine and lysine were the highest among the essential amino acids, with amounting to 0.67%, 0.70% and 0.48%, respectively, while those of serine, tyrosine and histidine were the highest among non-essential amino acids, with amounting to 0.69%, 0.66% and 0.44%, respectively. Except lysine and alanine, the other 15 kinds of amino acids in the leaves of pigeonpea generally increased along leaf age. The contents of total amino acids in the leaves were significantly different among different pigeonpea germplasms. Nineteen pigeonpea germplasms could be divided into four types by cluster analysis, such as low amino acid type (I), medium amino acid type (Ⅱ), medium and high amino acid type (Ⅲ) and high amino acid type (IV). Therefore, these would provide a theoretical basis for the processing of high-quality leaf tea and breeding of high-amino acid varieties of pigeonpea.

    参考文献
    [1] ZHONG X R. The value in use of semen cajan[J]. Res Inf Trad Chin Med, 2001, 3(8):47. doi:10.3969/j.issn.1673-4890.2001.08.023. 钟小荣. 木豆的利用价值[J]. 中药研究与信息, 2001, 3(8):47. doi:10.3969/j.issn.1673-4890.2001.08.023.
    [2] Zu Y G, Fu Y J, Liu W, et al. Simultaneous determination of four flavonoids in pigeonpea[Cajanus cajan (L.) Millsp.] leaves using RP-LC-DAD[J]. Chromatographia, 2006, 63(9/10):499-505. doi:10. 1365/s10337-006-0784-z.
    [3] FU Y J, ZU Y G, LIU W, et al. Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins[J]. J Chromatogr A, 2007, 1139(2):206-213. doi:10.1016/j.chroma.2006. 11.015.
    [4] ZHENG Y Y, YANG J, CHEN D H, et al. Effects of the extracts of Cajanus cajan L. on cell functions in human osteoblast-like TE85 cells and the derivation of osteoclast-like cells[J]. Acta Pharm Sin, 2007, 42(4):386-391. doi:10.3321/j.issn:0513-4870.2007.04.008. 郑元元, 杨京, 陈迪华, 等. 木豆叶提取物对人的类成骨细胞TE85成骨功能和体外破骨细胞分化的影响[J]. 药学学报, 2007, 42(4):386-391. doi:10.3321/j.issn:0513-4870.2007.04.008.
    [5] ZHANG J Y, QIU J, LI Z H, et al. The preliminary technological research on extraction of leaf protein concentrate from Cajanus cauan[J]. J SW For Coll, 2001, 21(1):40-44. doi:10.3969/j.issn.2095-1914. 2001.01.008. 张建云, 邱坚, 李正红, 等. 木豆叶蛋白提取工艺的初步研究[J]. 西南林学院学报, 2001, 21(1):40-44. doi:10.3969/j.issn.2095-1914. 2001.01.008.
    [6] DOU Z J, LIU H, LI M. Adaptability of pigeon pea (Cajanus cajan) introduced in the arid areas of Xinjiang[J]. Chin For Sci Technol, 2008, 22(2):60-63. doi:10.3969/j.issn.1000-8101.2008.02.017. 窦中江, 刘辉, 李铭. 新疆干旱区木豆引种适应性研究[J]. 林业科技开发, 2008, 22(2):60-63. doi:10.3969/j.issn.1000-8101.2008.02.017.
    [7] LIN M. A promising woody forage:Pigeonpea[J]. Feed Res, 1986(3):10. doi:10.13557/j.cnki.issn1002-2813.1986.03.005. 林茂. 一个有发展前途的木本饲料——木豆[J]. 饲料研究, 1986(3):10. doi:10.13557/j.cnki.issn1002-2813.1986.03.005.
    [8] CHEN Y X, ZHENG Z X, LI Z H. Tea from pigeonpea leaves and processing method:China, CN102630785A[P]. 2012-08-15. 陈昱璇, 郑子熙, 李振华. 一种木豆叶茶及其加工方法:中国, CN102630785A[P]. 2012-08-15.
    [9] RUAN Y C, WANG Y G. Biochemical basis on mellow, fresh, dense taste quality of green tea[J]. Tea Commun, 1987(4):1-4,15. 阮宇成, 王月根. 绿茶滋味品质醇、鲜、浓的生化基础[J]. 茶叶通讯, 1987(4):1-4,15.
    [10] WANG Y, LI C H. Studies on the variation of amino acid content and affecting factors in quality tea[J]. SW China J Agric Sci, 2006, 19(6):1121-1126. doi:10.3969/j.issn.1001-4829.2006.06.031. 王云, 李春华. 名优茶氨基酸含量变化规律及其影响因素研究[J]. 西南农业学报, 2006, 19(6):1121-1126. doi:10.3969/j.issn.1001-4829.2006.06.031.
    [11] FU Y P, ZHENG X B, LIU G S, et al. Studies on the changes in amino acids contents in flue-cured tobacco during leaf development[J]. J Chin Agric Univ, 1998, 3(5):95-96. 符云鹏, 郑宪滨, 刘国顺, 等. 烤烟叶片发育过程中氨基酸含量变化的研究[J]. 中国农业大学学报, 1998, 3(5):95-96.
    [12] ZHAO Z H, HE Y P. Studies on the changes in nutrient contents in leaves of Pugionium cornutum L. at different age[J]. Friends Farm, 2014(7):127-128. 赵志宏, 贺勇鹏. 沙芥不同叶龄营养成分含量变化规律的研究[J]. 农民致富之友, 2014(7):127-128.
    [13] ZHOU H L. Collecting and retention leaves of tea trees[J]. J Anhui Agric Sci, 1962(1):68-71. doi:10.13989/j.cnki.0517-6611.1962.01. 014. 周海龄. 茶树的採叶和留叶[J]. 安徽农业科学, 1962(1):68-71. doi:10.13989/j.cnki.0517-6611.1962.01.014.
    [14] CHEN Y H, LUO R H, WU Z K, et al. Analysis of genetic parameters and correlation in agronomic traits and quality characteristics in pigeonpea germplasm resources[J]. Guangxi Agric Sci, 2009, 40(11):1397-1402. doi:10.3969/j.issn.2095-1191.2009.11.002. 陈燕华, 罗瑞鸿, 吴子恺, 等. 木豆种质资源农艺与品质性状的相关性及遗传参数分析[J]. 广西农业科学, 2009, 40(11):1397-1402. doi:10.3969/j.issn.2095-1191.2009.11.002.
    [15] LUO G L, ZHOU Z G, LUO R H, et al. Analysis of agronomic and quality traits of pigeonpea CMS hybrids[J]. J Plant Gent Res, 2007, 8(4):481-485. doi:10.3969/j.issn.1672-1810.2007.04.021. 罗高玲, 周作高, 罗瑞鸿, 等. 木豆CMS杂交种资源农艺性状及品质性状分析[J]. 植物遗传资源学报, 2007, 8(4):481-485. doi:10. 3969/j.issn.1672-1810.2007.04.021.
    [16] ZHENG F Y, QIU S L, WU W J, et al. Study on the total flavonoids of the pigeonpea leaves in different growth periods[J]. Chin Agric Sci Bull, 2016, 32(28):110-115. doi:10.11924/j.issn.1000-6850.casb 16050062. 郑菲艳, 邱珊莲, 吴维坚, 等. 木豆不同生长期叶片总黄酮含量研究[J]. 中国农学通报, 2016, 32(28):110-115. doi:10.11924/j.issn. 1000-6850.casb16050062.
    [17] LIU X X, LI Z H, DENG J, et al. Study on protein content in pigeon pea leaves of different breeds and individuals[J]. Biomass Chem Eng, 2006, 40(4):19-21. doi:10.3969/j.issn.1673-5854.2006.04.006. 刘秀贤, 李正红, 邓疆, 等. 不同品种及单株木豆叶蛋白质含量的研究[J]. 生物质化学工程, 2006, 40(4):19-21. doi:10.3969/j.issn. 1673-5854.2006.04.006.
    引证文献
引用本文

徐晓俞,李爱萍,吴思逢,李程勋,黄旭旻,郑开斌.木豆不同品种和叶龄对叶片氨基酸形成的影响和聚类分析[J].热带亚热带植物学报,2018,26(6):617~626

复制
分享
文章指标
  • 点击次数:887
  • 下载次数: 718
  • HTML阅读次数: 298
  • 引用次数: 0
历史
  • 收稿日期:2018-02-28
  • 最后修改日期:2018-04-12
  • 在线发布日期: 2018-11-21
文章二维码