西南桦幼苗接种丛枝菌根真菌的生长与光合生理响应

Growth and Photosynthetic Physiology Response of Betula alnoides Seedlings to Inoculation of Arbuscular Mycorrhizal Fungi

摘要:

为了解丛枝菌根真菌（arbuscular mycorrhiza，AMF）对西南桦幼苗生长和光合生理的影响，对西南桦（Betula alnoides）优良无性系接种AMF菌株后的生长、光合参数、叶绿素含量和荧光参数进行了研究。结果表明，6个AMF菌株均能与西南桦无性系幼苗形成共生体，接种根内球囊霉（Glomus intraradices）菌株（AMF5）和摩西球囊霉（G. mosseae） HUN03B菌株（AMF3）显著提高了幼苗生长量、净光合速率、水分利用效率、叶绿素含量和荧光参数（P<0.05），显示出AMF5、AMF3与幼苗的亲和力明显优于其他菌株。西南桦4个无性系间的菌根侵染率差异不显著（P > 0.05），但AMF对无性系FB4、BY1的促生效应显著优于FB4+和A5。因此，适合与西南桦共生的优良菌株为AMF5和AMF3，这为西南桦菌根化育苗提供理论依据。

Abstract:

In order to understand the effect of arbuscular mycorrhizal fungi (AMF) on growth and photosynthetic physiology of Betula alnoides, four clone seedlings were inoculated with six arbuscular mycorrhizal fungi (AMF) and grown in greenhouse, and then seedling growth, photosynthetic parameters, chlorophyll contents and fluorescence parameters were investigated. The result showed that all six AMFs could form symbiote with seedlings of B. alnoides clones. After inoculated with Glomus intraradices (AMF5) and G. mosseae HUN03B strain (AMF3), the seedling growth, net photosynthetic rate, water use efficiency, chlorophyll contents and fluorescence parameters were significantly enhanced (P<0.05), indicating that affinity of AMF5 and AMF3 with clones was obviously better than that of other AMFs. There were no significant differences in inoculation rate among four clones, but the promotion effect of AMF inoculation on growth and photosynthetic physiology of FB4 and BY1 were obviously better than those of FB4+ and A5. Therefore, the optimal AMF strains were AMF5 and AMF3 for B. alnoides, which would provide theoretical foundation to its mycorrhizal seedling cultivation.