六角果鸢尾离体快繁技术体系的构建

Construction of Rapid Propagation System in vitro of Iris hexagona

摘要:

为构建六角果鸢尾（Iris hexagona）的离体快繁体系，以其幼嫩根状茎为外植体，研究了培养基和植物生长调节剂对不定芽诱导、增殖和植株生根的影响。结果表明，根状茎用0.1% HgCl₂消毒13 min的效果较佳；不定芽诱导最适培养基为MS+6-BA 1.5 mg L^-1+NAA 0.5 mg L^-1+蔗糖30 g L^-1+琼脂7.5 g L^-1，不定芽增殖的适宜培养基为MS+6-BA 0.5 mg L^-1+NAA 0.2 mg L^-1+KT 0.3 mg L^-1+蔗糖30 g L^-1+琼脂7.5 g L^-1；在MS+IBA 1.5 mg L^-1+蔗糖30 g L^-1+琼脂7.5 g L^-1培养基上不定芽生根率可达100%；腐殖土和珍珠岩+泥炭土+蛭石（1∶2∶1）均可作为组培苗移栽的适宜基质，移栽成活率可达100%。

Abstract:

In order to construct the rapid propagation system in vitro of Iris hexagona, the effects of medium and plant growth regulators on induction, proliferation and rooting of adventitious buds were studied by using young rhizome as explant. The results showed that the rhizome was sterilized for 13 min with 0.1% HgCl₂. The optimum medium for induction was MS+1.5 mg L^-1 6-BA+0.5 mg L^-1 NAA+30 g L^-1 sucrose+7.5 g L^-1 agar, and that for adventitious bud proliferation was MS+0.5 mg L^-1 6-BA+0.2 mg L^-1 NAA+0.3 mg L^-1 KT+30 g L^-1 sucrose+7.5 g L^-1 agar. The rooting rate of plantlets could reach up to 100% cultured on MS+1.5 mg L^-1 IBA+ 30 g L^-1 sucrose+7.5 g L^-1 agar. The survival of plantlets could reach up to 100% transplanted on humic soil or perlite+peat soil+vermiculite (1∶2∶1) as substrate.