超低温冷冻对羯布罗香种子结构和生理生化特性的影响

Effect of Cryopreservation on Structure, Physiology and Biochemical Characteristics of Dipterocarpus turbinatus Seeds

摘要:

为长期、稳定、有效地保存羯布罗香（Dipterocarpus turbinatus Gaertn. F.）种子，探讨了低温冷藏对种子活力、结构和生理生化指标的影响。结果表明，45%的含水量是种子的安全储藏含水量；玻璃化冷冻和缓慢冷冻对种子结构损害较大，且冷冻后的种子活力下降幅度大；快速冷冻法是种子超低温保存的最佳冷冻方式；液氮冷冻降低了种子过氧化物酶、过氧化氢酶和脱氢酶活性，提高了α-淀粉酶活性，而对丙二醛含量无显著影响。因此，超低温保存羯布罗香种子是科学可行的。

Abstract:

In order to preserve effectively and stably the seeds of Dipterocarpus turbinatus Gaertn. F., the influence of low temperature storage on seed vigor, structure and physiological and biochemical indexes was studied. The results showed that the water content of seeds with 45% was safe for storage. The vitrification and slow freezing had more damage to seed structure, and the seed vigor decreased greatly. So, the best method for cryopreservation of seeds was the rapid freezing. The liquid nitrogen frozen reduced the activities of POD, CAT and dehydrogenase, and increased the activity of α-amylase, but had no significant effects on the content of MDA. Therefore, ultralow temperature preservation is scientific and feasible method for the seeds of D. turbinatus.