桂花叶的化学成分及抗肿瘤活性研究

Study on Chemical Constituents and Antitumor Activities of Leaves of Osmanthus fragrans

摘要:

为了解桂花（Osmanthus fragrans）叶片的化学成分，应用多种色谱分离手段从桂花叶醇提溶液分离得到13个化合物，经波谱分析分别鉴定为香橙素（1）、染料木黄酮（2）、lupinalbin A（3）、齐墩果酸甲酯（4）、3-羰基齐墩果酸（5）、fouquierol（6）、19α-羟基-3-O-乙酰熊果酸（7）、白桦脂醇（8）、豆甾-3，5-二烯-7-酮（9）、刺五加酮（10）、对羟基苯乙酮（11）、3，16α-二羟基-对映贝壳杉烷（12）和trigoflavidone A（13）。所有化合物均为首次从桂花中分离得到。采用MTT法检测抗肿瘤活性表明，化合物2、3、10均有一定的抗肿瘤活性，且对4种肿瘤细胞株的活性检测为首次报道。

Abstract:

In order to understand the chemical constituents of Osmanthus fragrans leaves, thirteen compounds were isolated from the 95% ethanol extract. On the basis of spectral data, their structures were identified as aromadendrin (1), genistein (2), lupinalbin A (3), methy oleanolate (4), 3-ketooleanolic acid (5), fouquierol (6), 19α-hydroxyl acetylursolic acid (7), betulin (8), stigmasta-3,5-dien-7-one (9), ciwujiatone (10), p-hydroxyaceto-phenone (11), entkaurane-3,16α-diol (12), and trigoflavidone A (13). All of compounds are isolated from the leaves of O. fragrans for the first time. The compounds 2, 3, 10 showed significant antitumor activities, and their activity determination to 4 kinds of tumor cells was reported for the first time.