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SSR标记在桉树树种精准鉴定中的应用分析
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中央级公益性科研院所基本科研业务费专项资金（CAFYBB2016QA018）资助


Application of SSR Markers in Precise Identification of Eucalyptus Germplasms
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    摘要:

    为精准鉴定桉树种质，利用4个在种内完全保守的SSR位点对28种桉树（Eucalyptus）进行鉴定。根据这4个SSR位点的微卫星重复次数和侧翼序列特异核苷酸组合，构建了28种桉树种间种质资源的鉴定条码，能够精准鉴定9种桉树。这为桉树杂交育种工作提供了生物学依据。

    Abstract:

    In order to precise identification Eucalyptus germplasms, twenty-eight germplasms of Eucalyptus were classified by using 4 SSR loci with complete conservation sequence. According to the multiplicity of microsatellite and the specific bases in associated flanking region (FR) sequences, the identification barcodes among the 28 species of Eucalyptus were constructed, and 9 species of Eucalyptus were precisely identified. Therefore, these would provide the biological basis for hybrid breeding of Eucalyptus.

    参考文献
    [1] XIE Y J. Eucalyptus[M]. Beijing:China Forestry Publishing House, 2015:35-37. 谢耀坚. 真实的桉树[M]. 北京:中国林业出版社, 2015:35-37.
    [2] WILCOX M D. A Catalogue of the Eucalypts[M]. Auckland:Groome Poyry Ltd, 1997:114-116.
    [3] WILCOX M D. Supplement to a Catalogue of the Eucalypts[M]. Auckland:Groome Poyry Ltd, 1998:9-11.
    [4] QI S X. Eucalypt introduction and development status in China[J]. Guangxi For Sci, 2006, 35(4):250-252. doi:10.3969/j.issn.1006-1126.2006.04.016. 祁述雄. 中国引种桉树与发展现状[J]. 广西林业科学, 2006, 35(4):250-252. doi:10.3969/j.issn.1006-1126.2006.04.016.
    [5] GRIFFIN A R, BURGESS I P, WOLF L. Patterns of natural and manipulated hybridisation in the genus Eucalyptus L'hérit:A review[J]. Aust J Bot, 1988, 36(1):41-66. doi:10.1071/BT9880041.
    [6] QI S X. Eucalyptus in China[M]. 2nd ed. Beijing:China Forestry Publishing House, 2002:33-55. 祁述雄. 中国桉树[M]. 第2版. 北京:中国林业出版社, 2002:33-55.
    [7] HONG Y B, LIANG X Q, CHEN X P, et al. Construction of genetic linkage map in peanut (Arachis hypogaea L.) cultivars[J]. Acta Agron Sin, 2009, 35(3):395-402. doi:10.3724/SP.J.1006.2009.00395. 洪彦彬, 梁炫强, 陈小平, 等. 花生栽培种SSR遗传图谱的构建[J]. 作物学报, 2009, 35(3):395-402. doi:10.3724/SP.J.1006.2009.00395.
    [8] LIANG Y Q, ZHANG J J, LIANG J J, et al. Genetic diversity among persimmon germplasms in Henan Province[J]. Sci Silv Sin, 2015, 51(6):71-80. doi:10.11707/j.1001-7488.20150609. 梁玉琴, 张嘉嘉, 梁晋军, 等. 河南省柿种质资源的遗传多样性[J]. 林业科学, 2015, 51(6):71-80. doi:10.11707/j.1001-7488.20150609.
    [9] GUO Z D, XIA X Y, AN L J, et al. Genetic relationship analysis and cultivar identification of blueberry based on SSR markers[J]. J Plant Genet Resour, 2015, 16(5):1020-1026. doi:10.13430/j.cnki.jpgr.2015.05.013. 郭照东, 夏秀英, 安利佳, 等. 基于SSR标记的越橘亲缘关系分析及品种鉴定[J]. 植物遗传资源学报, 2015, 16(5):1020-1026. doi:10.13430/j.cnki.jpgr.2015.05.013.
    [10] ZHANG C L, GUO Z H, ZHANG X Q, et al. An analysis of species identification and genetic divergence of drooping wildrye (Elymus nutans) and siberian wildrye (E. sibiricus) by SSR markers[J]. J Plant Genet Resour, 2016, 17(3):416-422. doi:10.13430/j.cnki.jpgr.2016.03.003. 张成林, 郭志慧, 张新全, 等. 利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析[J]. 植物遗传资源学报, 2016, 17(3):416-422. doi:10.13430/j.cnki.jpgr.2016.03.003.
    [11] PRYOR L D, JOHNSON L A S. A Classification of the Eucalypts[M]. Canberra:The Australian National University, 1971:102.
    [12] HILL K D, JOHNSON L A S. Systematic studies in the Eucalypts:7. A revision of the bloodwoods, genus Corymbia (Myrtaceae)[J]. Telopea, 1995, 6(2/3):185-504. doi:10.7751/telopea19953017.
    [13] BROOKER M I H. A new classification of the genus Eucalyptus L'Hér. (Myrtaceae)[J]. Aust Syst Bot, 2000, 13(1):79-148. doi:10.1071/SB98008.
    [14] NICOLLE D. Classification of the Eucalypts (Angophora, Corymbia and Eucalyptus), Version 2[EB/OL]. (2015-04). http://www.dn.com.au/Classification-Of-The-Eucalypts.pdf.
    [15] DOYLE J J, DOYLE J L. Isolation of plant DNA from fresh tissue[J]. Focus, 1990, 12(1):13-15.
    [16] GAN S M, SHI J S, LI M, et al. Moderate-density molecular maps of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith genomes based on RAPD markers[J]. Genetica, 2003, 118(1):59-67. doi:10.1023/A:1022966018079.
    [17] LI F G. Linkage map construction and growth QTL detection in Eucalyptus urophylla and E. tereticornis based on STS markers[D]. Beijing:Chinese AcaAcademy of Forestry, 2010: 50–62. 李发根. 尾叶桉和细叶桉STS标记连锁图谱构建及生长性状QTL定位研究 [D]. 北京: 中国林业科学研究院, 2010: 50–62.
    [18] POSADA D, CRANDALL K A. MODELTEST: Testing the model of DNA substitution [J]. Bioinformatics, 1998, 14(9): 817–818. doi: 10. 1093/bioinformatics/14.9.817.
    [19] BARTHE S, GUGERLI F, BARKLEY N A, et al. Always look on both sides: Phylogenetic information conveyed by simple sequence repeat allele sequences [J]. PLoS One, 2012, 7(7): e40699. doi: 10.1371/ journal.pone.0040699.
    [20] HU T Y, LI X Q. Research on introduction of Encalyptus grandis and its normal region [J]. J Sichuan For Sci Technol, 1999, 20(4):8-13. doi:10.16779/j.cnki.1003-5508.1999.04.002. 胡天宇, 李晓清. 巨桉引种栽培及适生区域的研究[J]. 四川林业科技, 1999, 20(4):8-13. doi:10.16779/j.cnki.1003-5508.1999.04.002.
    [21] WANG H R. A Chinese Appreciation of Eucalypts[M]. Beijing:Science Press, 2010:1-16,93. 王豁然. 桉树生物学概论[M]. 北京:科学出版社, 2010:1-16,93.
    [22] DONG J X, WU C W. Towards horticultural and ornamental value of 27 introduced Eucalyptus species[J]. Yunnan For Sci Technol, 2003(4):39-41. doi:10.16473/j.cnki.xblykx1972.2003.04.007董静曦, 吴赤卫. 27种引种桉树的园艺观赏价值[J]. 云南林业科技, 2003(4):39-41. doi:10.16473/j.cnki.xblykx1972.2003.04.007
    [23] HAN Z, LIN J Y, HUANG Y F. The introduced resource and application on gardening virescence of Eucalypt in China[J]. Guang-dong For Sci Technol, 2007, 23(1):109-111. 韩宙, 林家怡, 黄永芳. 我国桉树引种资源及其在园林绿化上的应用[J]. 广东林业科技, 2007, 23(1):109-111.
    [24] WANG J Y. Development and characterization of microsatellite markers for phylogenetic analysis of Musa[D]. Haikou:Hainan University, 2010:43-71. 王静毅. SSR分子标记开发和在香蕉种质资源遗传分析中的应用[D]. 海口:海南大学, 2010:43-71.
    [25] LIU G, ZHANG D Q, XIE Y J, et al. Rapid screening and trans-ferability analysis of genomic-SSR and EST-SSR primers in Eucalypt[J]. Sci Silv Sin, 2013, 49(2):127-133. doi:10.11707/j.1001-7488.20130219. 刘果, 张党权, 谢耀坚, 等. 桉树genomic-SSR和EST-SSR引物的快速筛选与通用性研究[J]. 林业科学, 2013, 49(2):127-133. doi:10.11707/j.1001-7488.20130219.
    [26] MATSUOKA Y, MITCHELL S E, KRESOVICH S, et al. Micro- satellites in Zea: Variability, patterns of mutations, and use for evolu- tionary studies [J]. Theor Appl Genet, 2002, 104(2/3): 436–450. doi: 10. 1007/s001220100694.
    [27] SONNANTE G, CARLUCCIO A V, de PAOLIS A, et al. Identification of artichoke SSR markers: Molecular variation and patterns of diversity in genetically cohesive taxa and wild allies [J]. Genet Resour Crop Evol, 2008, 55(7): 1029–1046. doi: 10.1007/s10722-008-9310-5.
    [28] STEBBINS Jr G L. Variation and Evolution in Plants [M]. New York: Columbia University Press, 1950: 174–184.
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刘果,谢耀坚,吴志华,陈鸿鹏. SSR标记在桉树树种精准鉴定中的应用分析[J].热带亚热带植物学报,2018,26(2):116~124

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  • 收稿日期:2017-06-30
  • 最后修改日期:2017-09-13
  • 在线发布日期: 2018-03-29
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