SSR标记在桉树树种精准鉴定中的应用分析

Application of SSR Markers in Precise Identification of Eucalyptus Germplasms

摘要:

为精准鉴定桉树种质，利用4个在种内完全保守的SSR位点对28种桉树（Eucalyptus）进行鉴定。根据这4个SSR位点的微卫星重复次数和侧翼序列特异核苷酸组合，构建了28种桉树种间种质资源的鉴定条码，能够精准鉴定9种桉树。这为桉树杂交育种工作提供了生物学依据。

Abstract:

In order to precise identification Eucalyptus germplasms, twenty-eight germplasms of Eucalyptus were classified by using 4 SSR loci with complete conservation sequence. According to the multiplicity of microsatellite and the specific bases in associated flanking region (FR) sequences, the identification barcodes among the 28 species of Eucalyptus were constructed, and 9 species of Eucalyptus were precisely identified. Therefore, these would provide the biological basis for hybrid breeding of Eucalyptus.