黄皮种子中酰胺类生物碱及其杀线虫活性研究

Amide Alkaloids from the Seeds of Clausena lansium and Their Nematicidal Activities

摘要:

为了解黄皮种子中的酰胺类生物碱及其杀线虫活性，运用多种色谱学及波谱学方法分离并鉴定了10个酰胺类生物碱，分别为：N-甲基桂皮酰胺（1），clausenalansamide A （2），3-dehydroxy-3-methoxyl-clausenalansamide A （3），clausenalansamide B （4），黄皮新肉桂酰胺B （5），N-（2-苯乙基）肉桂酰胺（6），2'-dehydroxy-2'-oxo-clausenalansamide B （7），lansamide-7（8），homoclausenamide （9），1，5-dihydro-5-hydroxy-1-methyl-3，5-diphenyl-2H-pyrrol-2-one （10）。其中，化合物3，7，10为新天然产物。首次对黄皮种子中的酰胺类生物碱2~8进行全齿复活线虫致死活性的测试，发现所测化合物均有致死活性，其中，化合物2，3，5和8有较强的致死活性，且均优于阳性对照除线磷，可为相关农药的研发提供科学依据。

Abstract:

In order to understand the nematicidal activities of amide alkaloids from the seeds of Clausena lansium, ten amide alkaloids were isolated by comprehensive chromatographic techniques. On the basis of spectral data, their structures were identified as:N-methyl-cinnamamide (1), clausenalansamide A (2), 3-dehydroxy-3-methoxyl-clausenalansamide A (3), clausenalansamide B (4), lansiumamide B (5), N-2-phenylethyl-cinnamamide (6), 2'-dehydroxy-2'-oxo-clausenalansamide B (7), lansamide-7 (8), homoclausenamide (9), 1,5-dihydro-5-hydroxy-1-methyl-3,5-diphenyl-2H-pyrrol-2-one (10). Among them, compounds 3, 7 and 10 were new natural products. Meanwhile, the nematicidal activity against Panagrellus redivivusl was tested for the first time. All of them showed the nematicidal activity, and compounds 2, 3, 5 and 8 presented stronger activities than dichlorofenthion, providing scientific evidence for the development of new pesticide.