H2O2对蛇足石杉离体培养物诱变效应的研究

Studies on H2O2Induced Effect on Huperzia serrata in vitro

摘要:

为了获得高产石杉碱甲（Huperzine A，HupA）的蛇足石杉[Huperzia serrata（Thunb.） Trev.]叶状体，对H₂O₂诱变后的叶状体进行了研究。结果表明，诱变后叶状体株系的HupA含量显著提高，并获得高产株系SH42，其相对生长率和HupA含量分别达到4499.28%和261.17 μg g^–1DW，比起始叶状体分别提高了2.35倍和2.43倍；且株系间可溶性蛋白质谱带和SOD同工酶谱均存在差异，经过连续9代培养，变异叶状体可以稳定遗传。因此，H₂O₂对叶状体细胞具有良好的诱变效应，可以用于筛选高产HupA株系。

Abstract:

In order to gain high yield strain of huperzine A (HupA) from Huperzia serrata (Thunb.) Trev. in vitro, the hydrogen peroxide (H₂O₂) induced thallus was studied. The results showed that the HupA content in induced thallus significantly increased. The strain SH42 had highest yield of HupA with relative growth rate and HupA content for 4499.28% and 261.17 μg g^-1 DW, respectively, which increased 2.35 times and 2.43 times of the initial strain, respectively. Moreover, there were significant difference spectral band in SOD isozymes and soluble protein among strains. The variation characteristics were stable after nine generations. Therefore, it was suggested that H₂O₂ had good induced effect on thallus of Huperzia serrata, which could be used for screening high-yield strain of HupA in vitro.