遮荫对裸花紫珠苗期光合特性及总黄酮含量的影响
作者:
基金项目:

“十二五”国家科技支撑计划项目(2012BAD22B0504)资助


Effect of Shading on Photosynthetic Characteristics and Total Flavonoid Content of Callicarpa nudiflora Seedlings
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [45]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为了解遮荫对裸花紫珠(Callicarpa nudiflora)幼苗生长和药材质量的影响,研究了遮荫处理(遮荫30%、50%、70%和对照0%)下2年生裸花紫珠苗的光合气体交换参数、叶绿素荧光参数、光合色素和总黄酮含量的变化。结果表明,无遮荫或遮荫30%下,裸花紫珠的叶片净光合速率(Pn)日变化曲线呈典型的双峰型,而随遮荫程度的增加,Pn日变化曲线逐渐转变为单峰型。蒸腾速率(Tr)和气孔导度(Gs)日变化曲线呈单峰型,且峰值随着遮荫程度的增加而降低。PSⅡ最大光化学效率(Fv/Fm)和潜在活性(Fv/Fo)的日变化曲线呈“V”型,且两者均随遮荫程度的增加而升高。幼苗叶片的叶绿素a (Chl a)、叶绿素b (Chl b)、类胡萝卜素(Car)含量随遮荫程度的增加而显著增加(P < 0.05),而Chl a/b和Car/Chl却随遮荫程度的增加而下降。随遮荫程度的增加,叶片中总黄酮含量呈先上升后下降的趋势,以遮荫30%为最大。因此,裸花紫珠幼苗最适宜生长在无遮荫条件下,但为获得更高的总黄酮含量,可以选择30%遮荫处理。

    Abstract:

    In order to understand the effect of shading on growth and quality of Callicarpa nudiflora seedlings, the photosynthetic characteristics, chlorophyll fluorescence parameter, the content of chlorophyll and total flavonoid of two-year-old seedlings were studied under different shading treatments, including shading 30%, 50%, 70% and 0%, respectively. The results showed that the diurnal variation curve of net photosynthetic rate (Pn) of C. nudiflora was bimodal type under 0% and 30% shading, and with the increment of shading, diurnal variation curve of Pn was from bimodal into unimodal type gradually. The transpiration rate (Tr) and stomatal conductance (Gs) were unimodal type, and the peak value also gradually decreased with the increment of shading. The diurnal changes in PSⅡ maximal efficiency (Fv/Fm) and potential efficiency (Fv/Fo) showed ‘V’ type, those average value under shading treatments were significantly higher than those under 0% shading, and significantly increased (P < 0.05) with the increment of shade. The contents of chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoid (Car) in the leaves significantly increased with the increment of shading (P < 0.05), but the Chl a/b and Car/Chl decreased with the increment of shading, the content of total flavonoids in the leaves increased at first and then decreased, and reached up to maximum under shade of 30%. Therefore, from the photosynthetic characteristics, C. nudiflora seedling would be suitable for growth under 0% shade, but in order to gain high flavonoids, it would be a good choice under 30% shading.

    参考文献
    [1] Benvenuti S, Macchia M, Stefani A. Effects of shade on reproduction and some morphological characteristics of Abutilon theophrasti Medicos, Datura stramonium L. and Sorghum halepense L. Pers[J]. Weed Res, 1994, 34(4):283-288. doi:10.1111/j.1365-3180. 1994.tb01996.x.
    [2] DENG Y M, SHAO Q S, LI C C, et al. Differential responses of double petal and multi petal jasmine to shading:Ⅱ. Morphology, anatomy and physiology[J]. Sci Hort, 2012, 144:19-28. doi:10.1016/j.scienta. 2012.06.031.
    [3] CHAI S F, ZHUANG X Y, WEI X, et al. Effects of light intensity on photosynthesis and physiological characteristics in seedlings of Camellia pubipetala[J]. Acta Bot Boreali-Occid Sin, 2013, 33(3):547-554. doi:10.3969/j.issn.1000-4025.2013.03.018. 柴胜丰, 庄雪影, 韦霄, 等. 光照强度对濒危植物毛瓣金花茶光合生理特性的影响[J]. 西北植物学报, 2013, 33(3):547-554. doi:10.3969/j.issn.1000-4025.2013.03.018.
    [4] FAVARETTO V F, MARTINEZ C A, SORIANI H H, et al. Differential responses of antioxidant enzymes in pioneer and late-successional tropical tree species grown under sun and shade conditions[J]. Environ Exp Bot, 2011, 70(1):20-28. doi:10.1016/j.envexpbot.2010.06.003.
    [5] SONG Y, LIAO L, LIU T, et al. Response of photosynthesis and nitrogen distribution of Torreya grandis ‘Merrilli’ seedlings in different light regimes[J]. Sci Silv Sin, 2016, 52(5):55-63. doi:10.11707/j. 1001-7488.20160507. 宋洋, 廖亮, 刘涛, 等. 不同遮荫水平下香榧苗期光合作用及氮分配的响应机制[J]. 林业科学, 2016, 52(5):55-63. doi:10.11707/j. 1001-7488.20160507.
    [6] OSUNKOYA O O, ASH J E, HOPKINS M S, et al. Influence of seed size and seedling ecological attributes on shade-tolerance of rain-forest tree species in northern Queensland[J]. J Ecol, 1994, 82(1):149-163. doi:10.2307/2261394.
    [7] WU G L, CHEN M, DU G Z. Effects of nutrient and light on seedlings morphological plasticity of four Saussurea species with different ecological breadth[J]. Chin J Appl Ecol, 2008, 19(8):1708-1713. 武高林, 陈敏, 杜国祯. 营养和光照对不同生态幅风毛菊属植物幼苗形态可塑性的影响[J]. 应用生态学报, 2008, 19(8):1708-1713.
    [8] YAN X L, WANG D L. Effects of shading on the leaves and photosynthetic characteristics of Ligustrum robustum[J]. Acta Ecol Sin, 2014, 34(13):3538-3547. doi:10.5846/stxb201306241761. 闫小莉, 王德炉. 遮荫对苦丁茶树叶片特征及光合特性的影响[J]. 生态学报, 2014, 34(13):3538-3547. doi:10.5846/stxb201306241761.
    [9] ZHENG J, WU C H, CHEN Q X, et al. Influence of shading on growth and physiology of Dalbergia odorifera seedlings[J]. Sci Silv Sin, 2016, 52(12):50-57. doi:10.11707/j.1001-7488.20161206. 郑坚, 吴朝辉, 陈秋夏, 等. 遮荫对降香黄檀幼苗生长和生理的影响[J]. 林业科学, 2016, 52(12):50-57. doi:10.11707/j.1001-7488. 20161206.
    [10] DENG B, SHANG X L, FANG S Z, et al. Integrated effects of light intensity and fertilization on growth and flavonoid accumulation in Cyclocarya paliurus[J]. J Agric Food Chem, 2012, 60(25):6286-6292.
    [11] Editorial Committee of flora. Flora of China, Vol. 65[M]. Beijing:Science Press, 1982:37-38中国科学院植物志编辑委员会. 中国植物志, 第65卷[M]. 北京:科学出版社, 1982:37-38.
    [12] WANG Z N, HAN Z, CUI H B, et al. Chemical constituents from Callicarpa nudiflora[J]. J Trop Subtrop Bot, 2007, 15(4):359-362. doi:10.3969/j.issn.1005-3395.2007.04.014. 王祝年, 韩壮, 崔海滨, 等. 裸花紫珠的化学成分[J]. 热带亚热带植物学报, 2007, 15(4):359-362. doi:10.3969/j.issn.1005-3395.2007. 04.014.
    [13] ZHANG Q L, PANG Y X, YANG Q, et al. Herbal textual research of Callicarpa nudiflora[J]. J Guangdong Pharm Univ, 2014, 30(5):667-671. doi:10.3969/j.issn.1006-8783.2014.05.032. 张绮玲, 庞玉新, 杨全, 等. 裸花紫珠的本草考证[J]. 广东药学院学报, 2014, 30(5):667-671. doi:10.3969/j.issn.1006-8783.2014.05.032.
    [14] LIANG J J, QI J L, LI L, et al. Flavonoids from Callicarpa nudiflora leaves[J]. Chem Nat Comp, 2011, 47(1):110-111. doi:10.1007/s 10600-011-9846-z.
    [15] HUANG B, FU H Z, CHEN W K, et al. Hepatoprotective triterpenoid saponins from Callicarpa nudiflora[J]. Chem Pharm Bull, 2014, 62(7):695-699. doi:10.1248/cpb.c14-00159.
    [16] ZHENG D K, CHEN W K, MA S C, et al. Study on HPLC fingerprint of Callicarpa nudiflora and determination of ten components[J]. China J Chin Mat Med, 2015, 40(9):1776-1782. doi:10.4268/cjcmm 20150928. 郑东昆, 陈伟康, 马双成, 等. 裸花紫珠指纹图谱研究及10种成分的含量测定[J]. 中国中药杂志, 2015, 40(9):1776-1782. doi:10. 4268/cjcmm20150928.
    [17] ZHANG L, JIANG D Q, WENG C Y, et al. Study on the cutting technology of Callicarpa nudiflora, a medical plant used by the li people in Hainan[J]. Chin Agric Sci Bull, 2013, 29(13):197-200. 张浪, 姜殿强, 翁春雨, 等. 海南黎药裸花紫株扦插育苗技术研究[J]. 中国农学通报, 2013, 29(13):197-200.
    [18] ZHOU Z Z, LIU S C, ZHANG J H, et al. Effects of IBA treatment on adventitious rooting and endogenous hormones contents of shoot cuttings of Callicarpa nudiflora[J]. Chin J Trop Crops, 2016, 37(6):1075-1080. doi:10.3969/j.issn.1000-2561.2016.06.004. 周再知, 刘式超, 张金浩, 等. 外源IBA对裸花紫珠扦插生根和内源激素含量变化的影响[J]. 热带作物学报, 2016, 37(6):1075-1080. doi:10.3969/j.issn.1000-2561.2016.06.004.
    [19] GENTY B, BRIANTAIS J M, BAKER N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochim Biophys Acta, 1989, 990(1):87-92. doi:10.1016/S0304-4165(89)80016-9.
    [20] BILGER W, BJÖRKMAN O. Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absor-bance changes, fluorescence and photosynthesis in leaves of Hedera canariensis[J]. Photosynth Res, 1990, 25(3):173-185. doi:10.1007/BF00033159.
    [21] WU X M, YIN B Z. Determination of flavone from Ginkgo biloba leaves[J]. Chin J Pharm Anal, 2001, 21(2):138-141. 吴晓明, 殷斌志. 银杏叶黄酮类化合物的测定方法[J]. 药物分析杂志, 2001, 21(2):138-141.
    [22] VOLPI N, BERGONZINI G. Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry[J]. J Pharm Biomed Anal, 2006, 42(3):354-361. doi:10.1016/j.jpba.2006.04.017.
    [23] ZHAO S, HUANG Q X, LI Y L, et al. Effects of shading treatments on photosynthetic characteristics of Juniperus sabina Ant. seedlings[J]. Acta Ecol Sin, 2014, 34(8):1994-2002. doi:10.5846/stxb201306101606. 赵顺, 黄秋娴, 李玉灵, 等. 遮荫处理对臭柏幼苗光合特性的影响[J]. 生态学报, 2014, 34(8):1994-2002. doi:10.5846/stxb201306101606.
    [24] XIAO R L, WANG J R, SHAN W X, et al. Tea plantation environment and quality under different degrees of shading[J]. Chin J Eco-Agric, 2007, 15(6):6-11. 肖润林, 王久荣, 单武雄, 等. 不同遮荫水平对茶树光合环境及茶叶品质的影响[J]. 中国生态农业学报, 2007, 15(6):6-11.
    [25] YANG J X, GUO B L, LU R Q, et al. The effect of shading on the growth and photosynthesis characteristics in American blackberry[J]. Acta Hort Sin, 2005, 32(2):292-294. doi:10.3321/j.issn:0513-353X. 2005.02.020. 杨俊霞, 郭宝林, 鲁韧强, 等. 遮荫对美国黑莓生长及光合特性的影响[J]. 园艺学报, 2005, 32(2):292-294. doi:10.3321/j.issn:0513-353X.2005.02.020.
    [26] MAXWELL K, JOHNSON G N. Chlorophyll fluorescence:A practical guide[J]. J Exp Bot, 2000, 51(345):659-668. doi:10.1093/jxb/51.345. 659.
    [27] BAKER N R. Chlorophyll fluorescence:A probe of photosynthesis in vivo[J]. Annu Rev Plant Biol, 2008, 59:89-113. doi:10.1146/annurev. arplant.59.032607.092759.
    [28] TRUEMAN S J, RICHARDSON D M. Propagation and chlorophyll fluorescence of Camptotheca acuminata cuttings[J]. J Med Plants Res, 2011, 5(1):1-6. doi:10.3923/rjmp.2011.1.20.
    [29] LIU Y Q, SUN X Y, WANG Y, et al. Effects of shades on the photo-synthetic characteristics and chlorophyll fluorescence parameters of Urtica dioica[J]. Acta Ecol Sin, 2007, 27(8):3457-3464. doi:10.3321/j.issn:1000-0933.2007.08.044. 刘悦秋, 孙向阳, 王勇, 等. 遮荫对异株荨麻光合特性和荧光参数的影响[J]. 生态学报, 2007, 27(8):3457-3464. doi:10.3321/j.issn:1000-0933.2007.08.044.
    [30] WANG J H, REN S F, SHI B S, et al. Effects of shades on the photosynthetic characteristics and chlorophyll fluorescence parameters of Forsythia suspense[J]. Acta Ecol Sin, 2011, 31(7):1811-1817. 王建华, 任士福, 史宝胜, 等. 遮荫对连翘光合特性和叶绿素荧光参数的影响[J]. 生态学报, 2011, 31(7):1811-1817.
    [31] WEN G S, TIAN H T, ZHANG M R, et al. Application of chlorophyll fluorescence analysis in forest tree cultivation[J]. Chin J Appl Ecol, 2006, 17(10):1973-1977. 温国胜, 田海涛, 张明如, 等. 叶绿素荧光分析技术在林木培育中的应用[J]. 应用生态学报, 2006, 17(10):1973-1977.
    [32] XU D Q, ZHANG Y Z, ZHANG R X. Photoinhibition of photo-synthesis in plants[J]. Plant Physiol Commun, 1992, 28(4):237-243. 许大全, 张玉忠, 张荣铣. 植物光合作用的光抑制[J]. 植物生理学通讯, 1992, 28(4):237-243.
    [33] JIA H S, LI D Q, HAN Y Q. Advances in studies on photoinhibition in photosynthesis of higher plants[J]. Chin Bull Bot, 2000, 17(3):218-224. doi:10.3969/j.issn.1674-3466.2000.03.004. 贾虎森, 李德全, 韩亚琴. 高等植物光合作用的光抑制研究进展[J]. 植物学报, 2000, 17(3):218-224. doi:10.3969/j.issn.1674-3466. 2000.03.004.
    [34] WITTMANN C, ASCHAN G, PFANZ H. Leaf and twig photosynthesis of young beech (Fagus sylvatica) and aspen (Populus tremula) trees grown under different light regime[J]. Bas Appl Ecol, 2001, 2(2):145-154. doi:10.1078/1439-1791-00047.
    [35] DAI Y J, SHEN Z G, LIU Y, et al. Effects of shade treatments on the photosynthetic capacity, chlorophyll fluorescence, and chlorophyll content of Tetrastigma hemsleyanum Diels et Gilg[J]. Environ Exp Bot, 2009, 65(2/3):177-182. doi:10.1016/j.envexpbot.2008.12.008.
    [36] HUANG C J, WEI G, JIE Y C, et al. Effect of shade on plant traits, gas exchange and chlorophyll content in four ramie cultivars[J]. Photo-synthetica, 2016, 54(3):390-395. doi:10.1007/s11099-016-0186-x.
    [37] CORTELL J M, KENNEDY J A. Effect of shading on accumulation of flavonoid compounds in (Vitis vinifera L.) pinot noir fruit and extraction in a model system[J]. J Agric Food Chem, 2006, 54(22):8510-8520.
    [38] SU W H, ZHANG G F, LI X H, et al. Relationship between accumu-lation of secondary metabolism in medicinal plant and environmental condition[J]. Chin Trad Herb Drugs, 2005, 36(9):1415-1418. doi:10. 3321/j.issn:0253-2670.2005.09.052. 苏文华, 张光飞, 李秀华, 等. 植物药材次生代谢产物的积累与环境的关系[J]. 中草药, 2005, 36(9):1415-1418. doi:10.3321/j.issn:0253-2670.2005.09.052.
    [39] CUSHNIE T P T, LAMB A J. Antimicrobial activity of flavonoids[J]. Int J Antimicrob Agents, 2005, 26(5):343-356. doi:10.1016/j.ijanti micag.2005.09.002.
    [40] XU Y, WANG H L, WANG G B, et al. Effects of temperature and light intensity on flavonoid biosynthesis of ginkgo (Ginkgo biloba L.) leaves[J]. J CS Univ For Technol, 2016, 36(4):30-34. doi:10.14067/j.cnki. 1673-923x.2016.04.006. 徐友, 王欢利, 汪贵斌, 等. 温度和光照强度对银杏叶黄酮合成的影响[J]. 中南林业科技大学学报, 2016, 36(4):30-34. doi:10. 14067/j.cnki.1673-923x.2016.04.006.
    [41] LI Z L, GUO K X, ZHOU S B, et al. Effects of light intensity on biological characteristics, physiological indexes and flavone content of Kalimeris indica[J]. Acta Pratacult Sin, 2014, 23(4):162-170. doi:10. 11686/cyxb20140420. 李中林, 郭开秀, 周守标, 等. 光强对马兰形态、生理及黄酮类化合物含量的影响[J]. 草业学报, 2014, 23(4):162-170. doi:10.11686/cyxb20140420.
    [42] ZHU X F, ZHOU S B, YANG J H, et al. Effects on leaf characteristics and total flavone content of Dichondra repens under different light intensity[J]. Acta Laser Biol Sin, 2009, 18(1):62-66. doi:10.3969/j.issn.1007-7146.2009.01.012. 朱肖锋, 周守标, 杨集辉, 等. 不同光照强度对马蹄金叶的特征及总黄酮含量的影响[J]. 激光生物学报, 2009, 18(1):62-66. doi:10. 3969/j.issn.1007-7146.2009.01.012.
    [43] WANG H T, XIE B D, JIANG Y Z, et al. Effect of solar irradiation intensity on leaf development and flavonoid and terpene content in Ginkgo biloba leaves[J]. Acta Agric Univ Jiangxi (Nat Sci), 2002, 24(5):617-622. doi:10.3969/j.issn.1000-2286.2002.05.013. 王华田, 谢宝东, 姜岳忠, 等. 光照强度对银杏叶片发育及黄酮和内酯含量的影响[J]. 江西农业大学学报(自然科学版), 2002, 24(5):617-622. doi:10.3969/j.issn.1000-2286.2002.05.013.
    [44] WU Y Y, LIU Y H, LI Z, et al. Dynamic variety study on the biomass and nutrients of Poaulaca oleracea L. in different illumination intensities[J]. Sci Technol Food Ind, 2008, 29(6):102-103,106. 吴爰爰, 刘迎辉, 李震, 等. 马齿苋在不同光照强度下生物量及营养成分的动态变化研究[J]. 食品工业科技, 2008, 29(6):102-103, 106.
    [45] SUN S, LIU W G, PAN F S, et al. The effect of ecological conditions on flavonoid accumulation in Ginkgo biloba leaves[J]. J Plant Resour Environ, 1998, 7(3):1-7. 孙视, 刘晚苟, 潘福生, 等. 生态条件对银杏叶黄酮积累的影响[J]. 植物资源与环境学报, 1998, 7(3):1-7.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

李晨晨,周再知,周树平,黄桂华,梁坤南,刘炳妤,宋晓琛.遮荫对裸花紫珠苗期光合特性及总黄酮含量的影响[J].热带亚热带植物学报,2017,25(6):569~578

复制
分享
文章指标
  • 点击次数:1504
  • 下载次数: 1314
  • HTML阅读次数: 624
  • 引用次数: 0
历史
  • 收稿日期:2017-04-10
  • 最后修改日期:2017-07-15
  • 在线发布日期: 2017-12-04
文章二维码