短孢角毛藻相似种类的形态学和系统学研究
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作者单位:

华南师范大学生命科学学院,华南师范大学生命科学学院

基金项目:

国家自然科学基金项目(31370235,31570205);广东省科技计划项目(2015A030401084)资助


Morphological and Phylogenetic Studies on Chaetoceros brevis Schütt and Its Allied Taxa
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Affiliation:

South China Normal University,South China Normal University

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    摘要:

    短孢角毛藻的形态特征多变,容易导致物种的错误鉴定,并且其与若干相似种类之间存在物种混淆的情况。基于建立的单克隆培养藻株,结合生活史中形态特征的连续观察,以及基于目标基因序列分析的分子系统学数据,对短孢角毛藻(Chaetoceros brevis Schütt)及其相似种类的形态学和系统学进行了研究。丰富了短孢角毛藻休眠孢子的形态学描述,报道了一个新的特征:休眠孢子壳面上刺的末端具有帽状结构,该特征未报道于其他角毛藻种类,可作为短孢角毛藻的标志性特征。报道了我国角毛藻属的1个新记录种——拟短孢角毛藻(C.pseudobrevis Pavillard),该种具有独特的形态学特征,以及独立的系统学位置,应是一个独立的物种,而非短孢角毛藻的同种异名。垂缘组角毛藻的种类在系统发育树中显示出较好的单系起源,链端角毛及其走势是否可以作为该类群的共享特征,目前尚难做出定论。

    Abstract:

    Because of the variation of some key morphological characteristics and the subjective influences from researchers,the mis-identification has occurred commonly to Chaetoceros brevis.Meanwhile,there are still some debate on the species sharing very similar morphology with C.brevis,for example whether they could be ranked as a separated species or just a variety as C.brevis var.pseudobrevis.To solve the above questions,several monoclonal stains were established,their morphology and systematic were studied based on the combination of continuously morphological observation in the life cycle and molecular data from some target genes.One new morphological characteristic was found in C.brevis,i.e.,the cap-like structure on the top of spins on the surface of resting spore,which has never been reported in other Chaetoceros species,and could be a unique feature to identify C.brevis.A newly recorded diatom species for China was observed,C.pseudobrevis.Combine the morphology and molecular data,it was shown that C.pseudobrevis should be a separate taxa,not a variety of C.brevis.In the phylogenetic trees,the taxa in section Laciniosa clustered in the same clade,which shown the monophygeny and matching with the morphological grouping.But so far it is difficult to confirm the common-sharing features for this section.

    参考文献
    [1] ROUND F E, CRAWFORD R M, MANN D G. Diatoms:Biology and Morphology of the Genera[M]. Cambridge:Cambridge University Press, 1990:747-749.
    [2] HASLE G R, SYVERTSEN E E. Marine diatoms[M]//TOMAS C R. Identifying Marine Phytoplankton. London:Academic Press, 1997:5-387.
    [3] RINES J E B, HARGRAVES P E. The Chaetoceros Ehrenberg (Bacillariophyceae) flora of Narragansett Bay, Rhode Island, U.S.A.[J]. Bibl Phycol, 1988, 79(3):1-196.
    [4] JENSEN K G, MOESTRUP Ø. The genus Chaetoceros (Bacillario-phyceae) in inner Danish coastal waters[J]. Opera Bot, 1998, 18(1):88. doi:10.1111/j.1756-1051.1998.tb01103.x.
    [5] FRYXELL G A, HASLE G R. Taxonomy of harmful diatoms[M]//HALLEGRAEFF G M, ANDERSON D M, CEMBELLA A D. Manual on Harmful Marine Microalgae. 2nd ed. Paris, France:UNESCO Publishing, 2003:465-510.
    [6] GUIRY M D, GURIY G M. AlgaeBase[EB/OL]. Galway:World-Wide Electronic Publication, National University of Ireland. http://www.algaebase.org.
    [7] SCHÜTT F. Types of chaetoceros and peragallia:A contribution to the seas Flora[J]. Rep Ger Bot Soc, 1895, 13(4):35-50.
    [8] CUPP E E. Marine Plankton Diatoms of the West Coast of North America, Vol. 5[M]. UC San Diego:Scripps Institution of Oceano-graphy, 1943:1-237.
    [9] JIN D X, CHEN J H, HUANG K G. Marine Planktonic Diatoms from China Sea[M]. Shanghai:Science and Technology Press, 1965:115-118. 金德祥, 陈金环, 黄凯歌. 中国海洋浮游硅藻类[M]. 上海:上海科学技术出版社, 1965:115-118.
    [10] HERNÁNDEZ-BECERRIL D U. A morphological study of Chaeto-ceros species (Bacillariophyta) from the plankton of the Pacific Ocean of Mexico[J]. Bull Nat Hist Mus Lond (Bot), 1996, 26(1):1-73.
    [11] GUO Y J. Flora Algarum Marinarum Sinicarum, Tomus V. Bacilla-riophyta, No. 1 Centricae[M]. Beijing:Science Press, 2003:345-346. 郭玉洁. 中国海藻志, 第5卷(硅藻门)第1册(中心纲)[M]. 北京:科学出版社, 2003:344-346.
    [12] SUNESEN I, HERNÁNDEZ-BECERRIL D U, SAR E A. Marine diatoms from Buenos Aires coastal waters (Argentina):V. Species of the genus Chaetoceros[J]. Rev Biol Mar Oceanogr, 2008, 43(2):303-326. doi:10.4067/s0718-19572008000200009.
    [13] WANG Y, NIE R, Li Y, et al. Species diversity and geographical distribution of Chaetoceros in Guangdong coastal waters[J]. Adv Mar Sci, 2010, 28(3):342-352. doi:10.3969/j.issn.1671-6647.2010.03.010. 王艳, 聂瑞, 李扬, 等. 广东沿海角毛藻(Chaetoceros)的种类多样性及其地理分布[J]. 海洋科学进展, 2010, 28(3):342-352. doi:10. 3969/j.issn.1671-6647.2010.03.010.
    [14] PAVILLARD M J. Observations on the Diatomées[J]. Bull Soc Bot France, 1911, 58(1):21-29. doi:10.1080/00378941.1911.10831437.
    [15] GUILLARD R R L, HARGRAVES P E. Stichochrysis immobilis is a diatom, not a chrysophyte[J]. Phycologia, 1993, 32(3):234-236. doi:10.2216/i0031-8884-32-3-234.1.
    [16] KOOISTRA W H C F, SARNO D, HERNÁNDEZ-BECERRIL D U, et al. Comparative molecular and morphological phylogenetic analyses of taxa in the Chaetocerotaceae (Bacillariophyta)[J]. Phycologia, 2010, 49(5):471-500. doi:10.2216/09-59.1.
    [17] CHENG Z D, GAO Y H, LIU S C. Nano-Diatoms in Fujian Coast[M]. Beijing:Ocean Press, 1993:91-95. 程兆第, 高亚辉, 刘师成. 福建沿岸微型硅藻[M]. 北京:海洋出版社, 1993:91-95.
    [18] LUNDHOLM N, DAUGBJERG N, MOESTRUP Ø. Phylogeny of the Bacillariaceae with emphasis on the genus Pseudo-nitzschia (Bacilla-riophyceae) based on partial LSU rDNA[J]. Eurp J Phycol, 2002, 37(1):115-134. doi:10.1017/s096702620100347x.
    [19] SCHOLIN C A, HERZOG M, SOGIN M, et al. Identification of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae):Ⅱ. Sequence analysis of a fragment of the LSU rRNA gene[J]. J Phycol, 1994, 30(6):999-1011. doi:10.1111/j.0022-3646. 1994.00999.x.
    [20] NUNN G B, THEISEN B F, CHRISTENSEN B, et al. Simplicity-correlated size growth of the nuclear 28S ribosomal RNA D3 expansion segment in the crustacean order Isopoda[J]. J Mol Evol, 1996, 42(2):211-223. doi:10.1007/bf02198847.
    [21] HALL T A. BioEdit:a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT[J]. Nucl Acid Symp Ser, 1999, 41(2):95-98.
    [22] NYLANDER J A A. MrModeltest v2. Program[M/OL]. Uppsala:Evolutionary Biology Centre, Uppsala University, 2004. http://www.citeulike.org/user/mbutler808/article/7578084.
    [23] MILLER M A, PFEIFFER W, SCHWART T. Creating the CIPRES science gateway for inference of large phylogenetic trees[C]//Proceedings of 2010 Gateway Computing Environments Workshop (GCE). New Orleans, LA:IEEE, 2010:8.
    [24] RONQUIST F, TESLENKO M, van der MARK P, et al. MrBayes 3.2:Efficient Bayesian phylogenetic inference and model choice across a large model space[J]. Syst Biol, 2012, 61(3):539-542. doi:10.1093/sysbio/sys029.
    [25] HENDEY N I. An Introductory Account of the Smaller Algae of British Coastal Waters, Part V:Bacillariophyceae (Diatoms). MAFF Fishery Investigations, Series 4[M]. London:Her Majesty's Stationery Office, 1964:235-239.
    [26] HALLFORT G. Checklist of Baltic Sea Phytoplankton Species (Including Some Heterotrophic Protistan Groups)[M]. Helsinki:Helsinki Commission, Baltic Marine Environment Protection Commission, 2004:121-123.
    [27] EVENSEN D L, HASLE G R. The morphology of some Chaetoceros (Bacillariophyceae) species as seen in the electron microscopes[J]. Nov Hed Beih, 1975, 53(4):153-174.
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陈作艺,李扬.短孢角毛藻相似种类的形态学和系统学研究[J].热带亚热带植物学报,2017,25(4):399~408

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  • 收稿日期:2016-11-21
  • 最后修改日期:2017-03-06
  • 录用日期:2017-04-10
  • 在线发布日期: 2017-07-18
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