白菊木树皮的化学成分及其抗炎活性研究

Chemical Constituents from Gochnatia decora Barks and Their Anti-inflammatory Activities

摘要:

为了解白菊木[Gochnatia decora （Kurz） A.L.Cabrera]的化学成分，从其树皮甲醇提取物的石油醚萃取部位分离出11个化合物，分别鉴定为木栓酮（1）、木栓醇（2）、齐墩果酸（3）、齐墩果烯（4）、豆甾醇（5）、β-谷甾醇（6）、棕榈酸（7）、正十五烷酸（8）、十四烷酸-1-甘油酯（9）、对羟基苯甲酸甲酯（10）和没食子酸甲酯（11），所有化合物均为首次从白菊木属植物中发现。采用脂多糖（LPS）诱导小鼠巨噬细胞RAW264.7产生一氧化氮（NO）模型和中性粒细胞弹性蛋白酶抑制试验对单体化合物1~4进行了抗炎活性测试，结果表明，化合物1、3和4具有明显的NO抑制活性（IC₅₀<10 μmol L^-1），化合物2、3和4具有一定的中性粒细胞弹性蛋白酶抑制活性。

Abstract:

In order to clarify the chemical constituents and their anti-inflammatory activities of Gochnatia decora,eleven compounds were isolated from the petroleum ether fraction of methanol extracts from the barks of G.decora.These compounds were identified as friedelin (1),friedelinol (2),oleanolic acid (3),oleanene (4),stigmasterol (5),β-sitosterol (6),palmitic acid (7),n-pentadecanoic acid (8),l-O-myristoylglycerol (9),methyl 4-hydroxybenzoate (10),and methyl gallate (11).All the compounds were obtained from the genus Gochnatia for the first time.The compounds 1,3 and 4 displayed significantly high inhibitory activities against NO production (IC₅₀<10 μmol L^-1),while 2,3 and 4 exhibited moderate neutrophil elastase (NE) inhibitory activities by the assay of LPS-induced NO production in RAW264.7 cells and inhibition of NE,respectively.