基于ISSR方法的芦笋育种亲本间亲缘关系分析

Genetic Relationships among 19 Breeding Parents of Asparagus by ISSR

摘要:

为了解芦笋（Asparagus officinalis L.）育种亲本间的遗传关系，采用ISSR方法对19份芦笋育种亲本进行亲缘关系和遗传多样性分析。结果表明，18条引物共扩增出187条谱带，多态性比率达93.6%。UPGMA聚类分析表明，亲本的相似系数为0.56~0.80，在相似系数0.62处可将亲本分成3大类群，第Ⅰ类群为美国芦笋品种‘绿龙’；第Ⅱ类群由荷兰品种‘金林’人工培育的部分亲本和美国品种‘格兰德’组成；第Ⅲ类群为其余亲本组成。同一品种不同个体间也可能存在较大的遗传距离，芦笋经倍性育种培育出的个体表现出丰富的遗传多样性。这为芦笋杂交育种中的亲本筛选提供了理论依据。

关键词:芦笋;育种;亲缘关系;ISSR

Abstract:

In order to understand the genetic relationship among of Asparagus officinalis L. germplasms, the genetic diversity of 19 asparagus parents were analyzed by ISSR. The results showed that 187 bands were amplified by 18 primers with polymorphism rate for 93.6%. UPGMA indicated the Nei's coefficient ranged from 0.56 to 0.80. These cultivars could be clustered into three groups according to average coefficient of 0.62. Asparagus officinalis ‘Green Dragon’ cultivar from US formed GroupⅠwhich had far relation with others. Group II consisted of some artificial breeding parents of ‘Gijnlim’ from Holland and ‘Grande’ from US. The other tested parents made up the Group III. The individuals derived from the same variety had large genetic distance. There were abundant genetic diversity among individuals of asparagus from ploidy breeding. Therefore, these would provide the theoretical basis and technical guidance for parent selection in hybrid breeding of asparagus.

Key words:Asparagus;Breeding;Genetic relationship;ISSR