油茶砧和茶穗嫁接后苗期叶片形态和次级代谢物含量的变化

Changes in Morphological Characters and Secondary Metabolite Contents in Leaves of Grafting Seedlings with Camellia sinensis as Scions and C. oleifera as Stocks

摘要:

为了解茶/油茶嫁接苗叶片在形态和代谢产物上的变化，以茶‘舒茶早’品种（Camellia sinensis ‘Shuchazao’）为接穗，大别山野生油茶（C. oleifera）为砧木进行根颈嫁接，对嫁接体、茶树和油茶的叶片形态和次生代谢产物含量进行了研究。结果表明，茶/油茶嫁接体叶片在形态上更接近于茶，而嫁接体叶片形态学指标却受到了油茶的影响。嫁接体叶片中的氨基酸、嘌呤碱和多酚含量比油茶叶片高，其中茶氨酸含量比油茶显著提高。嫁接体叶片中含有酚酸，而在油茶中未被检出。嫁接体叶片中咖啡碱含量比茶树叶片显著下降。这对茶、油茶的栽培，茶树次级代谢及次级代谢物的转运机理研究具有一定的积极作用。

Abstract:

In order to understand the changes in morphology and metabolites in leaves of grafted tea seedlings, Camellia sinensis ‘Shuchazao’ as scions and C. oleifera (wild oil tea in Dabieshan) as stocks were grafted, and the morphology and secondary metabolite contents in their leaves were studied. The results showed that the morphology of grafted seedling leaves was more close to C. sinensis, and its morphological indexes of leaves were influenced by C. oleifera. The contents of amino acids, purine alkaloids and polyphenols in grafted seedling leaves greatly increased compared with those of C. oleifera. The content of theanine in grafted seedling leaves was significantly higher than that of C. oleifera. Moreover, phenolic acids were detected in grafted seedling leaves but not in leaves of C. oleifera. Caffeine content in grafted seedling leaves decreased obviously compared with that of C. sinensis. These results would produce positive impact on the cultivation of C. sinensis and C. oleifera, and play positive roles in the biosynthesis and transportation mechanisms of secondary metabolites in tea plants.