光质对白芨组培苗生理特性及酶基因表达的影响

Effect of Light Quality on Physiological Characteristics and Expression of Antioxidant Enzyme Genes in Bletilla striata L. in vitro

摘要:

为探讨光质对白芨（Bletilla striata）组培苗生长发育的影响，对不同光质下白芨组培苗的生长特征、抗氧化酶活性以及酶基因表达进行了研究。结果表明，蓝光和红光对白芨生长有显著促进作用，绿光的作用不明显。除了CAT外，不同光质处理白芨的APX、POD、SOD活性均呈上升趋势，且黄光处理的白芨SOD和APX活性最高，红光处理的POD活性最高，绿光处理的抗氧化酶活性比其他光质的低，蓝光处理35~45 d对抗氧化酶基因表达具有促进作用。因此，红光和黄光促进白芨生根组培苗的长高和生根；不同光质处理总体上提高了白芨氧化酶活性；白芨抗氧化酶基因的表达在蓝光处理下最大。

Abstract:

In order to understand the effect of light quality on growth of Bletilla striata seedlings in vitro, the growth characters, activities of antioxidant enzymes and enzyme gene expression of B. striata seedlings under different light qualities were studied. The results showed that there were significant promoting effect on growth of B. striata under blue light and red light, but the effect of green light was not obvious. Except of CAT, the activities of APX, POD and SOD increased under different light qualities, in which the activities of APX and SOD were the highest under yellow light, and that of POD did under red light. The activities of antioxidant enzyme under green light were the lowest. The expression of antioxidant enzyme genes increased under blue light for 35-45 days. Therefore, red and yellow light could promote the height and rooting of B. striata, the activities of antioxidant enzyme increased overall under different light qualities, and the expression of antioxidant enzyme genes were the highest under blue light.