毛竹7个新近发表种下类型的AFLP分析

Studies on the Relationship of Newly Published Infra-species of Phyllostachys edulis by AFLP Marker

摘要:

为了解毛竹（Phyllostachys edulis）种下类型的遗传关系，对花龟竹（P.edulis‘Mira’）、斑毛竹（‘Porphyrosticta’）、安吉锦毛竹（‘Anjiensis’）、麻衣竹（‘Exaurita’）、黄皮毛竹（‘Holochrysa’）、绿槽龟甲竹（‘Lücaoguijiazhu’）、孝丰紫筋毛竹（‘Purpureosulcata’）等7个新近发表的种下类型进行了AFLP分子标记分析。结果表明，采用筛选的8对引物组合共扩增了1728条条带，多态性条带百分率达98.77%，检测到多态性位点881个。Nei’s遗传多样性指数（H）为0.2736，Shannon信息指数（I）为0.4291。这丰富了毛竹所有23个种下类型的分子亲缘关系研究数据。

Abstract:

In order to understand the genetic relationship of Phyllostachys edulis, seven newly published infra-species, including‘Mira’,‘Porphyrosticta’,‘Anjiensis’,‘Exaurita’,‘Holochrysa’,‘Lücaoguijiazhu’, and‘Purpureosulcata’, were studied using AFLP molecular marker. The results showed that there were 1728 bands amplified by selected 8 pairs of primer with 98.77% polymorphic bands and 881 polymorphic loci. The Nei's genetic diversity index was 0.2736 and Shannon information index was 0.4291. These supplied the molecular research data of all 23 infra-species of P.edulis.