温敏核不育系培矮64S及其eui突变体长选3S最上节间蛋白质组差异分析

Proteomics Differences Analysis of the Uppermost Internode between TGMS Rice Line Pei'ai 64S and Its eui Mutant Changxuan 3S

摘要:

为从蛋白质表达水平了解长穗颈温敏核不育水稻（Oryza sativa）最上节间伸长机理，采用双向凝胶电泳方法对温敏核不育系培矮64S及其eui突变体长选3S抽穗前2 d的最上节间蛋白质进行了分离，并获得了分辨率和重复性较好的双向凝胶电泳图谱。选取50个差异蛋白质点进行MALDI-TOF-TOF-MS肽质谱指纹图谱分析，从中鉴定出31个已知蛋白质，相对于培矮64S，在长选3S中上调表达的11个蛋白质和下调表达的20个蛋白质。这些差异蛋白质按照其功能可分为7类。这些差异蛋白质可能与长选3S抽穗期最上节间剧烈伸长生长有关，水稻eui基因可能是通过调节抽穗期最上节间这些蛋白质的表达，从而控制最上节间细胞分裂，尤其是细胞的伸长生长。

Abstract:

In order to understand the elongation mechanism of the uppermost internode of TGMS rice (Oryza sativa L.) at protein expression level, the proteins in the uppermost internodes of eui mutant ‘Changxuan 3S’ and ‘Pei’ai 64S’ (control) before 2 days to heading were isolated by two-dimensional (2-DE) electrophoresis. A 2-DE map with a high resolution and good reproducibility were obtained by Coomasie brilliant blue staining. Fifty differentially expressed protein spots from 2-DE map were analyzed using MALDI-TOF/TOF-MS peptide mass fingerprint method, in which thirty-one differential proteins were identified. Compared with Pe'iai 64S, there were eleven proteins up-regulated in Changxuan 3S, and twenty proteins down-regulated. All of thirty-one differential proteins were divided into 7 categories according to their function. It was suggested that these differential proteins might play roles in elongation of the uppermost internode in ‘Changxuan 3S’ at heading stage, and eui gene in rice might regulate the expression of these proteins in uppermost internode at heading stage, thus promote cell division, especially cell elongation.