首页 > 过刊浏览>2016年第24卷第3期 >273-279. DOI:10.11926/j.issn.1005-3395.2016.03.005
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内源抗坏血酸对水稻种子萌发及幼苗生长的影响
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作者单位:

生命科学学院,生命科学学院,生命科学学院,生命科学学院,生命科学学院

基金项目:

国家自然科学基金项目(31270287,31570398);广东省自然科学基金重点项目(2015A030311023)资助


Effects of Endogenous Ascorbic Acid on Seed Germination and Seedling Growth of Rice
Author:
Affiliation:

School of Life Sciences,School of Life Sciences,School of Life Sciences,School of Life Sciences,School of Life Sciences

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    摘要:

    为了解内源抗环血酸在水稻(Oryza sativa)种子中的作用,以野生型品种‘中花11’(ZH-11)、抗坏血酸合成关键酶GLDH基因的上调(超表达)株系GO-2及下调(干涉)株系GI-2为材料,研究了抗环血酸含量对其种子萌发和幼苗生长的影响。结果表明,GO-2株系的种子萌发率比野生型ZH-11高6%,比干涉型GI-2高60%。外源添加1 mmol L-1抗坏血酸后,干涉型GI-2萌发率提高了22%,GO-2及ZH-11则没有明显增加。GO-2株系在幼苗的根长、株高、分蘖数和鲜重等指标上均高于ZH-11和GI-2。实时荧光定量测定结果表明,GO-2株系叶片的GLDH基因表达量显著上调,而GI-2株系则显著下调。这说明抗坏血酸有助于维持水稻种子活力和促进水稻种子发芽和幼苗生长。

    Abstract:

    The aim was to understand the function of endogenous ascorbic acid in rice (Oryza sativa) seeds. The homozygote transgenic lines GO-2 with the up-regulated GLDH (the key enzyme that catalyzes the final step of Asc biosynthesis in plants gene) and GI-2 with the down-regulated GLDH had been established from the cultivar ‘Zhonghua 11’ (ZH-11). The effect of endogenous ascorbic acid on seed germination and seedling growth of rice was studied among ZH-11, GO-2 and GI-2. The results showed that the germination rate of GO-2 was 6% and 60% higher than that of wild type (ZH-11), and GI-2, respectively. After treatment with 1 mmol L-1 exogenous ascorbic acid, the germination rate of GI-2 increased by 22%, but no significant effect was observed in that of GO-2 and the wild type (ZH-11). The root length, plant height, tiller number and fresh weight of seedling in GO-2 were higher than those in ZH-11 or GI-2. The expression of GLDH gene in GO-2 leaves was higher than that in ZH-11 or GI-2, too. So, these indicate ascorbic acid plays an important role in maintaining seed vigor and improving germination and seedling growth of rice.

    参考文献
    [1] MAPSON L W. Metabolism of ascorbic acid in plants: Part I. Function [J]. Annu Rev Plant Physiol, 1958, 9(1): 119-150. doi:10.1146/annurev.pp.09.060158.001003.
    [2] SHANG R G, WANG B Y. Effect of L-ascorbic acid on the germination of the old Maca (Lepidum meyenii Walp.) seeds [J]. Seed, 2013, 32(11): 38-41. doi:10.3969/j.issn.1001-4705.2013.11.011. 尚瑞广, 王兵益. 抗坏血酸对玛咖陈种子萌发的影响 [J]. 种子, 2013, 32(11): 38-41. doi:10.3969/j.issn.1001-4705.2013.11.011.
    [3] HUANG Z Y, OSBORNE D J. Endo-glycanhydrolases activities in Artemisia sphaerocephala (Asteraceae) mucilaginous achene germination process [J]. Acta Bot Sin, 2002, 44(6): 753-756.
    [4] LEON-KLOOSTERZIEL K M, van DE BUNT G A, ZEEVAART J A D, et al. Arabidopsis mutants with a reduced seed dormancy [J]. Plant Physiol, 1996, 110(1): 233-240. doi:10.1104/pp.110.1.233.
    [5] RAGHAVAN V. Induction of vivipary in Arabidopsis by silique culture: implications for seed dormancy and germination [J]. Amer J Bot, 2002, 89(5): 766-776. doi:10.3732/ajb.89.5.766.
    [6] FAN M H, ZHANG Y X, SHI G, et al. Effects of exogenous ascorbic acid on seed germination and growth of Brassica napus under seawater stress [J]. Chin J Oil Crop Sci, 2009, 31(1): 34-38. doi:10.3321/j.issn:1007-9084.2009.01.007. 范美华, 张义鑫, 石戈, 等. 外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响 [J]. 中国油料作物学报, 2009, 31(1): 34-38. doi:10.3321/j.issn:1007-9084.2009.01.007.
    [7] KE D S, SUN G C, WANG A G, et al. Relationship between ascorbic acid and germination of seeds [J]. Chin J Appl Environ Biol, 2003, 9(5): 497-500. doi:10.3321/j.issn:1006-687X.2003.05.012. 柯德森, 孙谷畴, 王爱国, 等. 抗坏血酸与种子萌发的关系 [J]. 应用与环境生物学报, 2003, 9(5): 497-500. doi:10.3321/j.issn:1006- 687X.2003.05.012.
    [8] YU L, LIU Y H, PENG X X. Cloning, prokaryotic expression of rice L-galactono-1,4-lactone dehydrogenase gene and preparation of anti-GLDH antibodies [J]. J Hunan Agri Univ (Nat Sci), 2010, 36(4): 381-384,429. doi:10.3724/SP.J.1238.2010.00381. 俞乐, 刘拥海, 彭新湘. 水稻L-半乳糖内酯脱氢酶基因的克隆和原核表达及抗体的制备 [J]. 湖南农业大学学报: 自然科学版, 2010, 36(4): 381-384,429. doi:10.3724/SP.J.1238.2010.00381.
    [9] GILLESPIE K M, AINSWORTH E A. Measurement of reduced, oxidized and total ascorbate content in plants [J]. Nat Protoc, 2007, 2(4): 871-874. doi:10.1038/nprot.2007.101.
    [10] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J]. Methods, 2001, 25(4): 402-408. doi:10.1006/meth.2001.1262.
    [11] WHEELER G L, JONES M A, SMIRNOFF N. The biosynthetic pathway of vitamin C in higher plants [J]. Nature, 1998, 393(6683): 365-369. doi:10.1038/30728.
    [12] CONKLIN P L. Recent advances in the role and biosynthesis of ascorbic acid in plants [J]. Plant Cell Environ, 2001, 24(4): 383-394. doi:10.1046/j.1365-3040.2001.00686.x.
    [13] YAN Q Z. Seed Science [M]. Beijing: China Agriculture Press, 2001: 91-118. 颜启传. 种子学 [M]. 北京: 中国农业出版社, 2001: 91-118.
    [14] SHEN Y H, XU Z J, LI W H, et al. Effects of vitamin C soaking on germination of alfalfa seeds under different salinity stress [J]. Seed, 2009, 28(7): 42-44. doi:10.3969/j.issn.1001-4705.2009.07.013. 申玉华, 徐振军, 李文辉, 等. 维生素C浸种对盐胁迫下紫花苜蓿种子发芽特性的影响 [J]. 种子, 2009, 28(7): 42-44. doi:10.3969/j. issn.1001-4705.2009.07.013.
    [15] YANG P, LUO Q X, ZHANG K K, et al. Effects of exogenous vitamin on germination of cucumber seed in salt stress [J]. J Changjiang Veget, 2010(7): 41-42. doi:10.3865/j.issn.1001-3547.2010.07.024. 杨萍, 罗庆熙, 张珂珂, 等. 外源维生素对盐胁迫下黄瓜种子萌发及幼苗生长的影响 [J]. 长江蔬菜, 2010(7): 41-42. doi:10.3865/j. issn.1001-3547.2010.07.024.
    [16] KHAN A, IQBAL I, SHAH A, et al. Alleviation of adverse effects of salt stress in Brassica (Brassica campestris) by pre-sowing seed treatment with ascorbic acid [J]. Amer-Euras J Agri Environ Sci, 2010, 7(5): 557-560.
    [17] JIANG X W, LI H Q, WANG J H. Physiological response of Scutellaria baicalensis seed germination and seedling to exogenous ascorbic acid under salt stress [J]. Plant Physiol J, 2015, 51(2): 166-170. doi:10.13592/j.cnki.ppj.2014.0501. 江绪文, 李贺勤, 王建华. 盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应 [J]. 植物生理学报, 2015, 51(2): 166-170. doi:10.13592/j.cnki.ppj.2014.0501.
    [18] DONG X H, SONG M. Reasons of seed inferior change and its prevention and restoration [J]. China Seed Ind, 2002(1): 39-40. doi:10.3969/j.issn.1671-895X.2002.01.025. 董新红, 宋明. 种子劣变的原因及其防止与修复 [J]. 中国种业, 2002(1): 39-40. doi:10.3969/j.issn.1671-895X.2002.01.025.
    [19] GALLIE D R. Increasing vitamin C content in plant foods to improve their nutritional value: Successes and challenges [J]. Nutrients, 2013, 5(9): 3424-3446. doi:10.3390/nu5093424.
    [20] GALLIE D R. The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth [J]. J Exp Bot, 2013, 64(2): 433-443. doi:10.1093/jxb/ers330.
    [21] MÜLLER-MOULÉ P, CONKLIN P L, NIYOGI K K. Ascorbate deficiency can limit violaxanthin de-epoxidase activity in vivo [J]. Plant Physiol, 2002, 128(3): 970-977. doi:10.1104/pp.010924.
    [22] SMIRNOFF N. The function and metabolism of ascorbic acid in plant [J]. Ann Bot, 1996, 78(6): 661-669. doi:10.1006/anbo.1996.0175.
    [23] TOKUNAGA T, MIYAHARA K, TABATA K, et al. Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for L-galactono-1,4-lactone dehydrogenase [J]. Planta, 2005, 220(6): 854-863. doi:10.1007/s00425-004-1406-3.
    [24] VELJOVIC-JOVANOVIC S D, PIGNOCCHI C, NOCTOR G, et al. Low ascorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system [J]. Plant Physiol, 2001, 127(2): 426-435. doi:10.1104/pp. 010141.
    [25] TABATA K, OBA K, SUZUKI K, et al. Generation and properties of ascorbic acid-deficient transgenic tobacco cells expressing antisense RNA for L-galactono-1,4-lactone dehydrogenase [J]. Plant J, 2001, 27(2): 139-148. doi:10.1046/j.1365-313x.2001.01074.x.
    [26] LIU Y H, YU L, WANG R Z. Level of ascorbic acid in transgenic rice for L-galactono-1,4-lactone dehydrogenase overexpressing or suppressed is associated with plant growth and seed set [J]. Acta Physiol Plant, 2011, 33(4): 1353-1363. doi:10.1007/s11738-010- 0669-5.
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张启雷,刘强,高辉,陆俐娜,彭长连.内源抗坏血酸对水稻种子萌发及幼苗生长的影响[J].热带亚热带植物学报,2016,24(3):273~279

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  • 收稿日期:2015-11-10
  • 最后修改日期:2015-12-16
  • 录用日期:2016-03-01
  • 在线发布日期: 2016-05-19
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