金鱼草中央细胞中线粒体DNA拷贝数的定量

Quantification of Mitochondrial DNA Copies in Central Cells of Antirrhium majus

摘要:

为探究金鱼草（Antirrhinum majus）中央细胞的线粒体DNA拷贝数，采用竞争型定量PCR技术进行了测定。结果表明，金鱼草中央细胞的体积为（61570±732）μm³，平均携带（783±25）个拷贝的线粒体DNA，并且中央细胞与卵细胞具有相似的体积/线粒体DNA拷贝数比值。推测金鱼草中央细胞包含如此高丰度线粒体DNA可能是为早期胚乳细胞的发育而储备的。

关键词:中央细胞;线粒体DNA;定量PCR;金鱼草

Abstract:

The mitochondrial DNA (mtDNA) copies of Antirrhinum majus were measured by quantitative PCR method. The results showed that the mean volume of central cell of A. majus was (61570±732) μm³ with (783±25) mtDNA copies. There were similar ratio of volume to mtDNA copy between central cell and egg cell. It was presumed that the abundant mtDNA in central cell might reserve for the early development of endosperm cells.

Key words:Central cell;mtDNA;Quantitative PCR;Antirrhinum majus