金鱼草中央细胞中线粒体DNA拷贝数的定量
作者:
作者单位:

西北大学生命科学学院,西北大学生命科学学院,西北大学生命科学学院,西北大学生命科学学院,西北大学生命科学学院

基金项目:

国家自然科学基金青年项目(31200234);陕西省教育厅省级重点实验室科研计划项目(14JS089)资助


Quantification of Mitochondrial DNA Copies in Central Cells of Antirrhium majus
Author:
Affiliation:

College of Life Science,Northwest University,College of Life Science,Northwest University,College of Life Science,Northwest University,College of Life Science,Northwest University,College of Life Science,Northwest University

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [17]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为探究金鱼草(Antirrhinum majus)中央细胞的线粒体DNA拷贝数,采用竞争型定量PCR技术进行了测定。结果表明,金鱼草中央细胞的体积为(61570±732)μm3,平均携带(783±25)个拷贝的线粒体DNA,并且中央细胞与卵细胞具有相似的体积/线粒体DNA拷贝数比值。推测金鱼草中央细胞包含如此高丰度线粒体DNA可能是为早期胚乳细胞的发育而储备的。

    Abstract:

    The mitochondrial DNA (mtDNA) copies of Antirrhinum majus were measured by quantitative PCR method. The results showed that the mean volume of central cell of A. majus was (61570±732) μm3 with (783±25) mtDNA copies. There were similar ratio of volume to mtDNA copy between central cell and egg cell. It was presumed that the abundant mtDNA in central cell might reserve for the early development of endosperm cells.

    参考文献
    [1] WANG D Y, ZHANG Q, LIU Y, et al. The levels of male gametic mitochondrial DNA are highly regulated in angiosperms with regard tomitochondrial inheritance[J]. Plant Cell, 2010, 22(7):2402-2416. doi:10.1105/tpc.109.071902.
    [2] PREUTEN T, CINCU E, FUCHS J, et al. Fewer genes than organelles:Extremely low and variable gene copy numbers in mitochondria of somatic plant cells[J]. Plant J, 2010, 64(6):948-959. doi:10.1111/j. 1365-313X.2010.04389.x.
    [3] CAI Q, GUO L, SHEN Z R, et al. Elevation of pollen mitochondrial DNA copy number by WHIRLY2:Altered respiration and pollen tube growth in Arabidopsis[J]. Plant Physiol, 2015, 169(1):660-673. doi:10.1104/pp.15.00437.
    [4] SHEAHAN M B, MCCURDY D W, ROSE R J. Mitochondria as a connected population:Ensuring continuity of the mitochondrial genome during plant cell dedifferentiation through massive mito-chondrial fusion[J]. Plant J, 2005, 44(5):744-755. doi:10.1111/j. 1365-313X.2005.02561.x.
    [5] ARIMURA S, YAMAMOTO J, AIDA G P, et al. Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution[J]. Proc Natl Acad Sci USA, 2004, 101(20):7805-7808. doi:10.1073/pnas.0401077101.
    [6] CUMMINS J. Mitochondrial DNA in mammalian reproduction[J]. Rev Reprod, 1998, 3(3):172-182. doi:10.1530/ror.0.0030172.
    [7] EBERT K M, LIEM H, HECHT N B. Mitochondrial DNA in the mouse preimplantation embryo[J]. J Reprod Fertil, 1988, 82(1):145-149. doi:10.1530/jrf.0.0820145.
    [8] WAI T, AO A, ZHANG X Y, et al. The role of mitochondrial DNA copy number in mammalian fertility[J]. Biol Reprod, 2010, 83(1):52-62. doi:10.1095/biolreprod.109.080887.
    [9] TAKANASHI H, OHNISHI T, MOGI M, et al. Studies of mito-chondrial morphology and DNA amount in the rice egg cell[J]. Curr Genet, 2010, 56(1):33-41. doi:10.1007/s00294-009-0277-3.
    [10] TIAN H Q, ZHU X Y. Reproductive Biology of Angiosperm[M]. Beijing:Science Press, 2012:1-255. 田惠桥, 朱学艺. 被子植物生殖生物学[M]. 北京:科学出版社, 2012:1-255.
    [11] BERGER F, GRINI P E, SCHNITTGER A. Endosperm:An integrator of seed growth and development[J]. Curr Opin Plant Biol, 2006, 9(6):664-670. doi:10.1016/j.pbi.2006.09.015.
    [12] SUN M X, YANG H Y, ZHOU C. A new method for embryo sac isolation and in situ fusion of egg and synergid protoplasts in Nicotiana tabacum[J]. Acta Bot Sin, 1993, 35(12):893-900. 孙蒙祥, 杨弘远, 周嫦. 分离烟草胚囊的新方法及诱导卵细胞与助细胞原生质体的原位融合[J]. 植物学报, 1993, 35(12):893-900.
    [13] CHEN L, ZHANG Y N, QIU Y L. et al. Isolation of embryo sac cells of lettuce (Lactuca sativa)[J]. Acta Bot Yunnan, 2010, 32(4):323-327. doi:10.3724/SP.J.1143.2010.09131. 陈琳, 张亚楠, 邱义兰, 等. 莴苣胚囊细胞分离[J]. 云南植物研究, 2010, 32(4):323-327. doi:10.3724/SP.J.1143.2010.09131.
    [14] GILLILAND G, PERRIN S, BLANCHARD K, et al. Analysis of cytokine mRNA and DNA:Detection and quantitation by competitive polymerase chain reaction[J]. Proc Natl Acad Sci USA, 1990, 87(7):2725-2729. doi:10.1073/pnas.87.7.2725.
    [15] OKUDA S, TSUTSUI H, SHⅡNA K, et al. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells[J]. Nature, 2009, 458(7236):357-361. doi:10.1038/nature07882.
    [16] FAURE J E, ROTMAN N, FORTUNÉ P, et al. Fertilization in Arabidopsis thaliana wild type:Developmental stages and time course[J]. Plant J, 2002, 30(4):481-488. doi:10.1046/j.1365-313X.2002. 01305.x.
    [17] MARUYAMA D, VÖLZ R, TAKEUCHI H, et al. Rapid elimination of the persistent synergid through a cell fusion mechanism[J]. Cell, 2015, 161(4):907-918. doi:10.1016/j.cell.2015.03.018.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

曾紫风,孙欢,郭雪,高龙,王丹阳.金鱼草中央细胞中线粒体DNA拷贝数的定量[J].热带亚热带植物学报,2016,24(4):452~455

复制
分享
文章指标
  • 点击次数:1864
  • 下载次数: 1581
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2015-11-04
  • 最后修改日期:2016-01-18
  • 录用日期:2016-05-04
  • 在线发布日期: 2016-07-15
文章二维码