福建泰宁野生铁皮石斛种群的ISSR亲缘关系分析

Genetic Relationships among Wild Dendrobium officinale Populations in Taining, Fujian by ISSR

摘要:

为了解铁皮石斛(Dendrobium officinale)种质间的亲缘关系,利用ISSR技术对34份铁皮石斛种质资源进行亲缘关系和遗传多样性分析。结果表明,9条ISSR引物在34份种质中共扩增出78条带,多态位点百分率达100%。UPGMA聚类分析表明,种质的相似系数为0.61~0.92,在相似系数0.626 处,福建省泰宁的野生铁皮石斛与栽培铁皮石斛分为两大类。泰宁野生铁皮石斛种群的Nei's基因多样性(H)和遗传分化系数(G_st)分别为0.3111和0.4609,均高于栽培种群(0.3056和0.4204),表明泰宁野生铁皮石斛具有较丰富的多样性和较高的种群分化系数。AMOVA分析表明,铁皮石斛种群内变异指数为74%,种群间变异指数为26%,表明不同种群间可能存在基因交流。这些为不同地域的野生铁皮石斛资源的有效保护及利用提供理论依据及技术参考。

关键词:铁皮石斛;亲缘关系;ISSR;遗传多样性

Abstract:

In order to understand the genetic relationships among germplasms of Dendroubium officinale, the genetic relationships and genetic diversity of 34 germplasms of D. officinale were analyzed by ISSR. The results showed that total 78 bands were amplified by 9 ISSR primers with percentage of polymorphic loci at 100%. UPGMA cluster analysis indicated that the similarity coefficient ranged from 0.61 to 0.92, and wild and cultivated D. officinale were divided into 2 groups at level of 0.626. The Nei's gene diversity (H) and coefficient of gene differentiation (G_st) of wild D. officinale from Fujian Taining was 0.3111 and 0.4609, respectively, which were higher than those of cultivated D. officinale (0.3056 and 0.4204, respectively), indicated that there were high diversity and gene differentiation among Taining D. officianle populations. AMOVA analysis showed that variation coefficients between populations and within populations were 24% and 76%, respectively, which indicated that there was gene flow among different populations. These would provide theoretical basis and reference for efficient protect and utilization of wild D. officinale germplasms.

Key words:Dendrobium officinale;Genetic relationship;ISSR;Genetic diversity