首页 > 过刊浏览>2016年第24卷第3期 >259-266. DOI:10.11926/j.issn.1005-3395.2016.03.003
PDF HTML阅读 XML下载 导出引用 引用提醒
福建泰宁野生铁皮石斛种群的ISSR亲缘关系分析
作者:
作者单位:

三明市农业科学研究院药用植物研究所,三明市农业科学研究院药用植物研究所,三明市农业科学研究院药用植物研究所,福建泰宁红石山生态农业科技有限公司,三明市农业科学研究院药用植物研究所,三明市农业科学研究院药用植物研究所,三明市农业科学研究院药用植物研究所

基金项目:

国家级星火计划项目(2014GA720032);福建省种业创新与产业化工程项目(FJZZZX-1540);福建省三明市科技计划项目(2014-N-3);三明市农业科学研究院院立项目(2013-Y-01)资助


Genetic Relationships among Wild Dendrobium officinale Populations in Taining, Fujian by ISSR
Author:
Affiliation:

The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian,;China;Fujian TaiNing Hongshishan ecological agriculture science and technology company LTD,TaiNing,fujian,;China,The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian,The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian,Fujian TaiNing Hongshishan ecological agriculture science and technology company LTD,The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian,The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian,The Institute of Medicinal Plant,Sanming Academy of Agricultural Science,Shaxian,Fujian

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [22]
    • |
  • 相似文献 [20]
    • | | |
  • 文章评论
    摘要:

    为了解铁皮石斛(Dendrobium officinale)种质间的亲缘关系,利用ISSR技术对34份铁皮石斛种质资源进行亲缘关系和遗传多样性分析。结果表明,9条ISSR引物在34份种质中共扩增出78条带,多态位点百分率达100%。UPGMA聚类分析表明,种质的相似系数为0.61~0.92,在相似系数0.626 处,福建省泰宁的野生铁皮石斛与栽培铁皮石斛分为两大类。泰宁野生铁皮石斛种群的Nei's基因多样性(H)和遗传分化系数(Gst)分别为0.3111和0.4609,均高于栽培种群(0.3056和0.4204),表明泰宁野生铁皮石斛具有较丰富的多样性和较高的种群分化系数。AMOVA分析表明,铁皮石斛种群内变异指数为74%,种群间变异指数为26%,表明不同种群间可能存在基因交流。这些为不同地域的野生铁皮石斛资源的有效保护及利用提供理论依据及技术参考。

    Abstract:

    In order to understand the genetic relationships among germplasms of Dendroubium officinale, the genetic relationships and genetic diversity of 34 germplasms of D. officinale were analyzed by ISSR. The results showed that total 78 bands were amplified by 9 ISSR primers with percentage of polymorphic loci at 100%. UPGMA cluster analysis indicated that the similarity coefficient ranged from 0.61 to 0.92, and wild and cultivated D. officinale were divided into 2 groups at level of 0.626. The Nei's gene diversity (H) and coefficient of gene differentiation (Gst) of wild D. officinale from Fujian Taining was 0.3111 and 0.4609, respectively, which were higher than those of cultivated D. officinale (0.3056 and 0.4204, respectively), indicated that there were high diversity and gene differentiation among Taining D. officianle populations. AMOVA analysis showed that variation coefficients between populations and within populations were 24% and 76%, respectively, which indicated that there was gene flow among different populations. These would provide theoretical basis and reference for efficient protect and utilization of wild D. officinale germplasms.

    参考文献
    [1] Chinese Pharmacopoeia Commissione. Chinese Pharmacopoeia, Part 1 [M]. Beijing: Chemical Industry Press, 2010: 265-266. 国家药典委员会. 中华人民共和国药典(一部) [M]. 北京: 化学工业出版社, 2010: 265-266.
    [2] Delectis Florae Reipublicae Popularis Sinicae, Agendae Academiae Sinicae Edita. Florae Reipublicae Popularis Sinicae, Tomus 19 [M]. Beijing: Science Press, 1999: 11-17. 中国科学院中国植物志编辑委员会. 中国植物志, 第19卷 [M]. 北京: 科学出版社, 1999: 11-17.
    [3] Yang C Y, Li X L, Wang Y Q, et al. ISSR Analysis of cultivated medicinal species of Dendrobium Sw. [J]. Chin Agri Sci Bull, 2011, 27(4): 148-152. 杨春勇, 李学兰, 王云强, 等. 人工栽培石斛的ISSR标记分析 [J]. 中国农学通报, 2011, 27(4): 148-152.
    [4] Li X, He T, Chun Z. Detecting genetic diversity of Dendrobium tissue culture seedlings with ISSR method [J]. Chin J Appl Environ Biol, 2013, 19(2): 249-254. doi:10.3724/SP.J.1145.2013.00249. 李霞, 何涛, 淳泽. 石斛组培苗遗传差异性ISSR检测 [J]. 应用与环境生物学报, 2013, 19(2): 249-254. doi:10.3724/SP.J.1145.2013. 00249.
    [5] Jiang J L, Ye W, Li Y Q, et al. Growth and polysaccharides accumulation in autotetraploid Dendrobium officinale [J]. Plant Physiol J, 2014, 50(4): 519-526. 江金兰, 叶炜, 李永清, 等. 同源四倍体铁皮石斛的生长及多糖积累 [J]. 植物生理学报, 2014, 50(4): 519-526.
    [6] Shen Y, Xu C, Wan X F, et al. Application of ISSR-PCR to identification of different Dendrobium Sw. species [J]. Chin Trad Herb Drugs, 2005, 36(3): 423-427. doi:10.3321/j.issn:0253-2670.2005. 03.041. 沈颖, 徐程, 万小凤, 等. ISSR-PCR在石斛种间鉴别中的应用 [J]. 中草药, 2005, 36(3): 423-427. doi:10.3321/j.issn:0253-2670.2005. 03.041.
    [7] Shen J. The applications of DNA sequences and inter-simple sequence repeats for authenticating herbs [D]. Nanjing: Nanjing Normal University, 2005: 44-54. 沈洁. DNA序列、ISSR标记在药用植物种质鉴别中的应用 [D]. 南京: 南京师范大学, 2005: 44-54.
    [8] Shen Y. Studies of ISSR molecular marker and ITS sequences analysis in Dendrobium plants [D]. Hangzhou: Zhejiang University, 2004: 56-61. 沈颖. 石斛类ISSR标记研究与ITS序列分析 [D]. 杭州: 浙江大学, 2004: 56-61.
    [9] Lu J S, Bu Z Y, LÜ W L, et al. Analysis on genetic diversity of 20 plants of Orchidaceae by ISSR molecular marker [J]. SW China J Agri Sci, 2012, 25(6): 2252-2257. doi:10.3969/j.issn.1001-4829.2012. 06.060. 卢家仕, 卜朝阳, 吕维莉, 等. 20份兰科植物的ISSR遗传多样性分析 [J]. 西南农业学报, 2012, 25(6): 2252-2257. doi:10.3969/j.issn. 1001-4829.2012.06.060.
    [10] Lu J S, Bu Z Y, LÜ W L, et al. ISSR analysis on genetic diversity of germplasms resources in Dendrobium Sw. from different habitats [J]. Chin Trad Herb Drugs, 2013, 44(1): 96-100. doi:10.7501/j.issn.0253- 2670.2013.01.018. 卢家仕, 卜朝阳, 吕维莉, 等. 不同产地石斛属种质资源的ISSR遗传多样性分析 [J]. 中草药, 2013, 44(1): 96-100. doi:10.7501/j. issn.0253-2670.2013.01.018.
    [11] Shen J, Ding X Y, Ding G, et al. Studies on population difference of Dendrobium officinale: Ⅱ. Establishment and optimization of the method of ISSR fingerprinting mark [J]. China J Chin Mat Med, 2006, 31(4): 291-294. doi:10.3321/j.issn:1001-5302.2006.04.006. 沈洁, 丁小余, 丁鸽, 等. 铁皮石斛居群差异的研究: Ⅱ. ISSR指纹标记方法的建立与优化 [J]. 中国中药杂志, 2006, 31(4): 291-294. doi:10.3321/j.issn:1001-5302.2006.04.006.
    [12] Shen J, Xu H J, Yuan Y H, et al. Evaluation of genetic diversity of Dendrobium officinale wild population based on RAMP markers [J]. Acta Pharmac Sin, 2011, 46(9): 1156-1160. 沈洁, 徐慧君, 袁英惠, 等. 铁皮石斛野生居群基于RAMP标记的遗传多样性评价 [J]. 药学学报, 2011, 46(9): 1156-1160.
    [13] Shen J, Ding X Y, Liu D Y, et al. Intersimple sequence repeats (ISSR) molecular fingerprinting markers for authenticating populations of Dendrobium officinale Kimura et Migo [J]. Biol Pharm Bull, 2006, 29(3): 420-422. doi:10.1248/bpb.29.420.
    [14] Ma J M, Yin S H. Genetic diversity of Dendrobium fimbriatum (Orchidaceae), an endangered species, detected by inter-simple sequence repeat (ISSR) [J]. Acta Bot Yunnan, 2009, 31(1): 35-41. doi:10.3724/SP.J.1143.2009.08076. 马佳梅, 殷寿华. 西双版纳地区流苏石斛遗传多样性的ISSR分析 [J]. 云南植物研究, 2009, 31(1): 35-41. doi:10.3724/SP.J.1143.2009. 08076.
    [15] Zeng S H, Xiao F H, Zha Y H, et al. Preliminary evaluation among Dendrobium officinale populations in Yunnan wenshan [J]. Lishizhen Med Mat Med Res, 2012, 23(2): 468-470. doi:10.3969/j.issn.1008- 0805.2012.02.097. 曾淑华, 萧凤回, 查应洪, 等. 云南文山铁皮石斛居群的初步评价 [J]. 时珍国医国药, 2012, 23(2): 468-470. doi:10.3969/j.issn.1008- 0805.2012.02.097.
    [16] Li X X, Ding X Y, Chu B H, et al. Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP [J]. Genetica, 2008, 133(2): 159-166. doi:10.1007/s10709-007-9196-8.
    [17] Qian X, Wang C X, Tian M. Genetic diversity and population differentiation of Calanthe tsoongiana, a rare and endemic orchid in China [J]. Int J Mol Sci, 2013, 14(10): 20399-20413. doi:10.3390/ijms141020399.
    [18] Yao X H, Gao L, Yang B. Genetic diversity of wild Cymbidium goeringii (Orchidaceae) populations from Hubei based on inter-simple sequence repeats analysis [J]. Front Biol China, 2007, 2(4): 419-424. doi:10.1007/s11515-007-0064-9.
    [19] Wu H F, Li Z Z, Huang H W. Genetic differentiation among natural population of Gastrodia elata (Orichidaceae) in Hubei and germplasm assessment of the cultivated populations [J]. Biodiv Sci, 2006, 14(4): 315-326. doi:10.1360/biodiv.060053. 吴会芳,李作渊,黄宏文. 湖北野生天麻的遗传分化及栽培天麻种质评价 [J]. 生物多样性,2006, 14(4): 315-326. doi:10.1360/biodiv.060053.
    [20] Lü Y L, Chen Z, Zhuang X Q. A floristic analysis of Orchidaceae in Fujian Prorince, China [J]. J Nanjing For Univ (Nat Sci), 2009, 33(6): 162-165. 吕月良, 陈璋, 庄西卿. 福建省野生兰科植物区系研究 [J]. 南京林业大学学报: 自然科学版, 2009, 33(6): 162-165.
    [21] Editorial Board of Fujian Flora, Science and Technology Commission in Fujian Province. Flora Fujian [M]. Fuzhou: Fujian Science and Technology Press, 1995: 613-614. 福建省科学技术委员会《福建植物志》编辑委员会. 福建植物志 [M]. 福州: 福建科学技术出版社, 1995: 613-614.
    [22] Lin J L. Studies on distibution and habitat investigation of wild Dendrobium in Fujian [J]. For Prosp Des, 2009(2): 13-16. doi:10. 3969/j.issn.1004-2180.2009.02.004. 林建丽. 福建省野生石斛属植物分布及生境调查研究 [J]. 林业勘察设计, 2009(2): 13-16. doi:10.3969/j.issn.1004-2180.2009. 02.004.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

江金兰,叶炜,李永清,范志敏,雷伏贵,张锐,周建金.福建泰宁野生铁皮石斛种群的ISSR亲缘关系分析[J].热带亚热带植物学报,2016,24(3):259~266

复制
分享
文章指标
  • 点击次数:1961
  • 下载次数: 1786
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2015-07-13
  • 最后修改日期:2015-09-11
  • 录用日期:2015-11-18
  • 在线发布日期: 2016-05-19
文章二维码
您是第16711181 位访问者
主管单位：中国科学院
主办单位：中国科学院华南植物园 广东省植物学会
地址：广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部
电话：86 (0)20-3725 2514
热带亚热带植物学报 ® 2025 版权所有