棕囊藻北部湾株的18S rDNA分子鉴定

Molecular Identification of Phaeocystis from Beibu Gulf Based on 18S rDNA Sequences

摘要:

为研究北部湾棕囊藻(Phaeocystis)藻华的成因,采用PCR克隆了棕囊藻北部湾株核糖体18S rDNA序列。结果表明,棕囊藻北部湾株具有游动单细胞与群体结构两种形态;其18S rDNA序列和NCBI基因库中球形棕囊藻(Phaeocystis globosa)的同源性为99%~100%,在系统进化树上与不同海域来源的球形棕囊藻聚在一大分支上,且与球形棕囊藻间的遗传距离均小于其他种。首次从分子生物学上确定棕囊藻北部湾株为球形棕囊藻。

Abstract:

In order to understand the causes of algae bloom in Beibu Gulf, the sequences of ribosome 18S rDNA of Phaeocystis were cloned by PCR. The results showed that the strains from Beibu Gulf had single-celled and group structures, and their sequences of ribosome 18S rDNA showed 99%-100% homology to those Phaeocystis globosa in NCBI GenBank. The strains from Beibu Gulf and Phaeocystis globosa with different origins get together in a big clade in the phylogenetic tree, the genetic distance with Phaeocystis globosa was less than that with other species of Phaeocystis. Therefore, it was confirmed for the first time that the strains of Phaeocystis in Beibu Gulf caused algal bloom belonged to Phaeocystis globosa.