龙眼采后果肉劣变的差异表达蛋白分析

Analysis of Differential Expression Proteins during Postharvest Deterioration Process of Longan Aril

摘要:

为探索龙眼(Dimocarpus longan)果肉常温劣变过程中蛋白质的变化, 采用蛋白质组学的方法, 在‘福眼’龙眼果肉常温劣变过程中发现共有24 个2 倍差异表达蛋白, 其中21 个蛋白被成功鉴定。这些蛋白参与了应激反应与防御(56%)、能量与碳代谢(19%)、初级代谢(5%)、蛋白转运(5%)和细胞骨架(5%)等细胞代谢活动。大多数与抗氧化作用密切相关的蛋白质都下调表达, 说明龙眼采后果肉的抗氧化能力有所下降, 不能有效地缓解ROS 积累引起的毒性作用, 从而使果肉劣变。此外, 与能量代谢相关的蛋白全部下调表达, 表明龙眼果实采后果肉劣变可能与能量供应不足有关。这些结果为龙眼采后保鲜技术研究提供科学依据。

Abstract:

The aim was to investigate the changes in proteins during postharvest deterioration process of longan (Dimocarpus longan) under normal temperature. The results showed that twenty-four differentially expressed proteins in longan aril were detected from cultivar ‘Fuyan’ by using proteomics technique during deteriorating process, of which 21 proteins were successfully identified. Based on their physiological functions, all identified proteins could be involved in stress response and defense (accounting for 56%), energy and carbon metabolism (19%), primary metabolites pathway (5%), protein transport (5%), cytoskeleton (5%) and some other metabolic processes. Among them, most proteins related to antioxidant system were down-regulated. It was implied that the ability of antioxidant of longan aril was decreased in postharvest, which could not effectively alleviate the toxicity caused by ROS accumulation. In addition, the enzymes associated with energy metabolism were also downregulated, suggesting that the deterioration of postharvest longan aril might be related to the insufficient of energy supply. So, these results will provide a scientific basis for studying preservation technology of postharvest longan.