云南芒果种质基因组大小测定与变异分析
作者:
作者单位:

云南省热带作物科学研究所,云南省热带作物科学研究所,云南省热带作物科学研究所,云南省热带作物科学研究所

基金项目:

农业部热带作物种质资源保护项目(14RZZY-41, 15RZZY-41)资助


Genome Size and Variation Analysis of Mango (Mangifera indica L.) Germplasms in Yunnan by Flow Cytometry
Author:
Affiliation:

Yunnan Institute of Tropical Crops,Yunnan Institute of Tropical Crops,Yunnan Institute of Tropical Crops,Yunnan Institute of Tropical Crops

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [19]
  • |
  • 相似文献 [20]
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    为了解云南芒果(Mangifera indica L.)种质资源的基因组的变异情况, 采用流式细胞术对35 份云南芒果种质资源的基因组大小进行了测定和变异分析。结果表明, 云南芒果种质资源的基因组大小存在一定差异, 基因组的平均C值是0.445110 pg, 0.4353177×109 bp, 最小的是采自景洪的半栽培种YSM-44 (0.434567 pg, 0.4250060×109 bp), 最大的是采自红河的野生种YSM-25 (0.458679 pg, 0.4485881×109 bp)。基因组C值变异程度最大的是野生种(CV=1.65%), 其次为半野生种(CV=1.26%)、半栽培种(CV=1.21%)和栽培种(CV=0.11%)。与芒果具有相近基因组大小的多为苔藓植物, 与"C值悖论"观点相一致。因此, 应用流式细胞术能准确、快捷地测定芒果基因组大小, 而且云南野生、半野生及半栽培芒果种质资源遗传变异类型丰富, 有较大的挖掘利用潜力。

    Abstract:

    In order to understand the variation of mango (Mangifera indica L.) germplasms in Yunnan, the genome size of 35 germplasms was determined by flow cytometry and their variation was analyzed. The results showed that the mean genome size among the 35 germplasms was 0.445110 pg and 0.4353177×109 bp, which the minimum one (0.434567 pg, 0.4250060×109 bp) was YSM-44 from Jinghong, and the maximum one (0.458679 pg, 0.44485881×109 bp) was YSM-25 from Honghe. The genome size variation of wild germplasms was the largest (CV=1.65%), followed by semi-wild germplasms (CV=1.26%), semi-cultivated germplasms (CV=1.21%) and cultivated germplasms (CV=0.11%). The bryophytes had similar genome size to mango, which is consistent with the "C-value paradox" theory. Therefore, flow cytometry method could accurately and fastly measure genome size of mango, and the genetic variation in wild, semi-wild and semi-cultivated germplasms was rich, these could be used for mango breeding.

    参考文献
    [1] Mo R, Luo Y H, Zhou S M, et al. Polyembryony in mango (Mangifera indica L.) and genetic analysis [J]. J Trop Subtrop Bot, 2005, 13(6): 475-479. 莫饶, 罗远华, 周世民, 等. 几个芒果品种的胚性及多胚苗遗传分析 [J]. 热带亚热带植物学报, 2005, 13(6): 475-479.
    [2] Ma W H, Yao Q S, Sun G M. Study on diversity of important economic traits of mango fruits in the mango gene pool [J]. Chin J Trop Crops, 2005, 26(3): 7-11. 马蔚红, 姚全胜, 孙光明. 芒果种质资源果实重要经济性状多样性分析 [J]. 热带作物学报, 2005, 26(3): 7-11.
    [3] Wang Y, Jin Z Q, Chen Y Y, et al. Construction of AFLP technological system in Mangifera indica L. [J]. J Anhui Agri Sci, 2009, 37(33): 16305-16307. 王园, 金志强, 陈业渊, 等. 芒果AFLP分子标记体系的建立及应用 [J]. 安徽农业科学, 2009, 37(33): 16305-16307.
    [4] Lei X T, Chen Y Y, Zhang J B, et al. Research progress on molecular markers of Mangifera indica Linn. germplasm resources[J]. J Anhui Agri Sci, 2009, 37(32): 15722-15724,15850. 雷新涛, 陈业渊, 张建斌, 等. 芒果种质资源分子标记研究进展[J]. 安徽农业科学, 2009, 37(32): 15722-15724,15850.
    [5] Lei X T, Yao Q S, Xu X R, et al. The wild mango germplasm resources in China and their AFLP molecular markers [J]. Chin J Trop Crops, 2009, 30(10): 1408-1412. 雷新涛, 姚全胜, 徐雪荣, 等. 中国野生芒果种质资源及其AFLP 分子标记 [J]. 热带作物学报, 2009, 30(10): 1408-1412.
    [6] Fan W G, Luo Y, Wu S F, et al. Morphological characteristics and distribution of wild germplasm resources of Mangifera indica in south and north Pan River Valley [J]. SW China J Agri Sci, 2012, 25(6): 2244-2247. 樊卫国, 罗燕, 吴素芳, 等. 南北盘江河谷野生芒果种质资源的分布与形态特征 [J]. 西南农业学报, 2012, 25(6): 2244-2247.
    [7] Zheng M, Min T L. Anacardiaceae [M]// Flora Reipublicae Popularis Sinicae, Tomus 45(1). Beijing: Science Press, 1980: 74-78. 郑勉, 闵天禄. 漆树科 [M]// 中国植物志, 第45卷第1分册. 北京: 科学出版社, 1980: 74-78.
    [8] Ni Z G, Xie D H, Yu Y C, et al. Present situation and suggestions of mango industry in Yunnan [J]. Chin J Trop Agri, 2009, 29(6): 52-56. 尼章光, 解德宏, 俞艳春, 等. 云南芒果产业现状及对策 [J]. 热带农业科学, 2009, 29(6): 52-56.
    [9] Ni Z G, Zhang L H, Luo X P, et al. Investigation and analysis of the wild mango (Mangiferal indica L.) germplasm resources in Nujiang Low and Hot River Valley [J]. SW China J Agri Sci, 2008, 21(2): 436-439. 尼章光, 张林辉, 罗心平, 等. 怒江低热河谷芒果种质资源调查与分析 [J]. 西南农业学报, 2008, 21(2): 436-439.
    [10] Beaulieu J M, Leitch I J, Patel S, et al. Genome size is a strong predictor of cell size and stomatal density in angiosperms [J]. New Phytol, 2008, 179(4): 975-986.
    [11] Gruner A, Hoverter N, Smith T, et al. Genome size is a strong predictor of root meristem growth rate [J/OL]. J Bot, 2010, 2010: Article ID 390414 [2014-05-20]. http://dx.doi.org/10.1155/ 2010/390414.
    [12] Biémont C. Genome size evolution: Within-species variation in genome size [J]. Heredity, 2008, 101(4): 297-298.
    [13] Petrov D A. Evolution of genome size: New approaches to an old problem [J]. Trends Genet, 2001, 17(1): 23-28.
    [14] Royal Botanic Gardens, Kew. Plant DNA C-values Database, Ver. 6.0 [EB/OL]. (2012-12-01) [2014-07-20]. http://data. kew.org/cvalues.
    [15] Friedrich O. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA [J]. Method Cell Biol, 1990, 33(1): 105-110.
    [16] Liu J, Kong G H, Ni S B, et al. Estimation of genomic C value of Macadamia integrifolia spp. by flow cytometry [J]. Chin Agri Sci Bull, 2013, 29(34): 96-101. 柳觐, 孔广红, 倪书邦, 等. 基于流式细胞术的澳洲坚果基因组 C值测定 [J]. 中国农学通报, 2013, 29(34): 96-101.
    [17] Fang J G, Liu D J, Ma Z Q. Constructing mango (Mangifera indica L.) genetic map using markers for double heterozygous loci [J]. Mol Plant Breed, 2003, 1(3): 313-319. 房经贵, 刘大钧, 马正强. 利用双杂合位点标记资料构建芒果遗传图谱 [J]. 分子植物育种, 2003, 1(3): 313-319.
    [18] Greilhuber J, Doležel J, Lysák M, et al. The origin, evolution and proposed stabilization of the terms ‘genome size' and ‘C-value' to describe nuclear DNA content [J]. Ann Bot, 2005, 95(1): 255-260.
    [19] Bennett M D, Leitch I J. The origin, genome size evolution in plants [M]// Gregory T R. The Evolution of the Genome. San Diego: Elsevier, 2005: 89-162.
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

柳觐,李开雄,孔广红,倪书邦.云南芒果种质基因组大小测定与变异分析[J].热带亚热带植物学报,2015,23(4):386~390

复制
分享
文章指标
  • 点击次数:2026
  • 下载次数: 2517
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2014-10-24
  • 最后修改日期:2014-12-26
  • 录用日期:2015-03-09
  • 在线发布日期: 2015-07-15
文章二维码