云南芒果种质基因组大小测定与变异分析

Genome Size and Variation Analysis of Mango (Mangifera indica L.) Germplasms in Yunnan by Flow Cytometry

摘要:

为了解云南芒果(Mangifera indica L.)种质资源的基因组的变异情况, 采用流式细胞术对35 份云南芒果种质资源的基因组大小进行了测定和变异分析。结果表明, 云南芒果种质资源的基因组大小存在一定差异, 基因组的平均C值是0.445110 pg, 0.4353177×10⁹ bp, 最小的是采自景洪的半栽培种YSM-44 (0.434567 pg, 0.4250060×10⁹ bp), 最大的是采自红河的野生种YSM-25 (0.458679 pg, 0.4485881×10⁹ bp)。基因组C值变异程度最大的是野生种(CV=1.65%), 其次为半野生种(CV=1.26%)、半栽培种(CV=1.21%)和栽培种(CV=0.11%)。与芒果具有相近基因组大小的多为苔藓植物, 与"C值悖论"观点相一致。因此, 应用流式细胞术能准确、快捷地测定芒果基因组大小, 而且云南野生、半野生及半栽培芒果种质资源遗传变异类型丰富, 有较大的挖掘利用潜力。

关键词:芒果;种质资源;基因组;变异

Abstract:

In order to understand the variation of mango (Mangifera indica L.) germplasms in Yunnan, the genome size of 35 germplasms was determined by flow cytometry and their variation was analyzed. The results showed that the mean genome size among the 35 germplasms was 0.445110 pg and 0.4353177×10⁹ bp, which the minimum one (0.434567 pg, 0.4250060×10⁹ bp) was YSM-44 from Jinghong, and the maximum one (0.458679 pg, 0.44485881×10⁹ bp) was YSM-25 from Honghe. The genome size variation of wild germplasms was the largest (CV=1.65%), followed by semi-wild germplasms (CV=1.26%), semi-cultivated germplasms (CV=1.21%) and cultivated germplasms (CV=0.11%). The bryophytes had similar genome size to mango, which is consistent with the "C-value paradox" theory. Therefore, flow cytometry method could accurately and fastly measure genome size of mango, and the genetic variation in wild, semi-wild and semi-cultivated germplasms was rich, these could be used for mango breeding.

Key words:Mango;Germplasms;Genome size;Variation analysis