云南莲瓣兰主栽品种SSR指纹图谱的构建和遗传差异分析

Construction of SSR Fingerprint and Genetic Variance Analysis on Cymbidium tortisepalum Cultivars in Yunnan Province

摘要:

为了解云南莲瓣兰(Cymbidium tortisepalum)的遗传多样性,利用SSR 技术对32 个莲瓣兰主栽品种进行遗传变异分析,并构建莲瓣兰栽培品种的指纹图谱.结果表明,筛选出的12 对多态性高、稳定性好的引物共检测到95 个等位基因,每对引物检测到4~18 个等位基因,有效等位基因数(N_E)为61.489,平均有效等位基因数(N_A)为5.124,Shannon 信息指数(I)和多态性信息含量(PIC)分别为0.806~2.624 和0.789~ 0.953.12 对引物中,以引物SSR03 的等位基因数、N_E、观测杂合度、I 和PIC 最高.32 个品种在12 对引物上都具有不同的特异性条带,可以彼此区别.从12 对引物中筛选出3 对核心引物SSR02、SSR03 和SSR12 构建了莲瓣兰主栽品种SSR 分子指纹图谱,这3 对核心引物组合即可鉴定32 个莲瓣兰栽培品种.这为莲瓣兰的品种鉴定、遗传多样性分析和分子育种研究提供理论基础和技术支持.

Abstract:

In order to understand the genetic diversity of Cymbidium tortisepulum, the fingerprints of 32 C. tortisepulum cultivars were constructed by SSR. The results showed that there were 95 alleles were detected by selected 12 pairs of primers, the number of alleles selected by one primers ranged from 4 to 18, and the number of mean alleles (N_A) and effective alleles (N_E) were 61.489 and 5.124, respectively. The Shannon information index (I) and polymorphism information content values (PIC) of 32 cultivars ranged from 0.806 to 2.624, and 0.789 to 0.953, respectively. Among 12 paris of primer, the primer SSR03 was the highest in N_A, N_E, observed heterozygosity (H_O), I and PIC. All of 32 cultivars had different specific bands by 12 pairs of primer. The SSR molecular fingerprints of 32 cultivars were built by 3 core pairs of primers, including SSR02, SSR03 and SSR12. All of 32 cultivars could be distinguished each other by combined primers of SSR02 + SSR03 + SSR123. These would provide theoretical basis and technical support for studying in the identification of C. tortisepalum cultivars, genetic diversity analysis and molecular breeding.