薯瘟菌对甘薯叶片光合特性及叶绿体结构的影响
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福建省农业科学院,福建省农业科学院,福建省农业科学院,福建省农林大学生命科学院,福建省农林大学生命科学院

基金项目:

福建省自然科学基金项目(2008J04009);福建省农业科学院原生蔬菜科技创新团队项目(CXTD-2-1320)资助


Photosynthetic Characteristics and Chloroplast Ultrastructure of Sweet Potato Leaves Infected by Ralstonia solanacearum
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Fujian Academy of Agricultural Sciences,Zhangzhou Fujian,Fujian Academy of Agricultural Sciences,Zhangzhou Fujian,Fujian Academy of Agricultural Sciences,Zhangzhou Fujian,College of Life Science,Fujian Agriculture and Forestry University,College of Life Science,Fujian Agriculture and Forestry University

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    摘要:

    为探讨甘薯(Dioscorea esculenta)被薯瘟菌(Ralstonia solanacearum)侵染后的响应,以抗病品种‘湘薯75-55'和感病品种‘胜利百号'为材料,对甘薯叶片的光合特性和叶绿体结构进行了研究。结果表明,薯瘟菌侵染后,‘胜利百号'叶片的细胞膜透性(MP)明显增大,叶绿素(Chl)含量下降,净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)下降,胞间二氧化碳浓度(Ci)和内源ABA 含量急剧升高,且细胞膜完整性被破坏,叶绿体数目减少、膨胀模糊、基粒片层松散解体以及淀粉粒变小;而‘湘薯75-55'的各个指标变化不大,细胞膜结构完整,叶绿体基粒片层排列整齐。因此,薯瘟菌侵染后不同抗病品种甘薯叶片在光合特性和叶绿体结构上表现出显著的差异。

    Abstract:

    In order to understand the response of sweet potato (Dioscorea esculenta) to Ralstonia solanacearum, two varieties, including resistant ‘Xiangshu 75-55' and sensitive ‘Shenglibaihao’, were used as materials, the changes in photosynthetic characteristics, abscisic acid (ABA) content and chloroplast ultrastructure were studied after infected by R. solanacearum. The results showed that the membrane permeability (MP), intercellular CO2 concentration (Ci) and endogenous ABA content in ‘Shenglibaihao’ infected by R. solanacearum increased markedly, but chlorophyll (Chl) content, net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) obviously decreased. Besides, the integrity of chloroplast membrane system was damaged, the number of chloroplast decreased, and expansion to collapse, the grana lamella loosed and disintegrated, and starch grains got smaller in ‘Shenglibaihao’ leaves affected. In contrast, for ‘Xiangshu 75-55', the changes in the related physiological indexes were not different significantly, and the plasma membrane and lamellar still kept integration. Therefore, there were distinct differences in the PMP, Chl content, ABA content, Pn and chloroplast structure between the two sweet potato varieties with different disease-resistance.

    参考文献
    [1] Liu Z H, Yu H, Fang S M, et al. Preliminary study on the two different pathotypes of Ralsionia solanacearum [J]. Fujian J Agri Sci, 2011, 26(6): 1016-1020. 刘中华, 余华, 方树民, 等. 甘薯瘟两种不同致病型的初步研究[J]. 福建农业学报, 2011, 26(6): 1016-1020.
    [2] Fang S M, Chen Y S, Guo X D. Identifying and screening sweet potato varieties resistance to bacterial wilt and fusarium wilt diseases [J]. Sci Plant Gene Res, 2001, 2(1): 37-39. 方树民, 陈玉森, 郭小丁. 甘薯兼抗薯瘟病和蔓割病种质筛选鉴 定 [J]. 植物遗传资源科学, 2001, 2(1): 37-39.
    [3] Yuan Z N, Chen X Y, Zhang Z J, et al. Screening of the molecular markers linked to type I bacterial wilt resistant gene of sweet potato [J]. Acta Agri Univ Jiangxi, 2005, 27(6): 861-863. 袁照年, 陈选阳, 张招娟, 等. 甘薯抗I型薯瘟病的RAPD标记筛 选 [J]. 江西农业大学学报, 2005, 27(6): 861-863.
    [4] Wang W, Ruan M H, Qiu Y X, et al. Phenylaprapanoid metabolism of sweet potato against Pseudomonas solanacearum [J]. Chin J Eco-Agri, 2009, 17(5): 944-948. 王伟, 阮妙鸿, 邱永祥, 等. 甘薯抗薯瘟病的苯丙烷类代谢研究[J]. 中国生态农业学报, 2009, 17(5): 944-948.
    [5] Ruan M H, Wang W, Qiu Y X, el at. Changes of salicylic acid and reactive oxygen species metabolism in sweet potato against Pseudomonas solanacearum [J]. Chin J Trop Crops, 2008, 29(1): 14-17. 阮妙鸿, 王伟, 邱永祥, 等. 甘薯抗薯瘟病的水杨酸与活性氧的 代谢变化 [J]. 热带作物学报, 2008, 29(1): 14-17.
    [6] Zou Q. Plant Physiological and Biochemical Experiments [M]. Beijing: Chinese Agricultural Press, 1995: 1-108. 邹琦. 植物生理生化实验指导 [M]. 北京: 中国农业出版社, 1995: 1-108.
    [7] Zhang M S, Xie B, Tan F. Relationship between changes on endogenous hormone of sweet potato under water stress and drought resistance [J]. Scientia Agricultura Sinica, 2002, 35(5): 498-501. 张明生, 谢波, 谈锋. 水分胁迫下甘薯内源激素的变化与品种抗 旱性的关系 [J]. 中国农业科学, 2002, 35(5): 498-501.
    [8] Li Z T, Zhou X. Plant Hormones and Immune Detection Technology[M]. Nanjing: Jiangsu Science and Technology Press, 1996: 1-345. 李宗霆, 周燮. 植物激素及其免疫检测技术 [M]. 南京: 江苏科 学技术出版社, 1996: 1-345.
    [9] Su J W, Wang X P. Cadmium-induced membrane lipid peroxidation and programmed cell death in tea seedling [J]. J Plant Physiol Mol Biol, 2002, 28(4): 292-298. 苏金为, 王湘平. 镉诱导的茶树苗膜脂过氧化和细胞程序性死 亡 [J]. 植物生理与分子生物学学报, 2002, 28(4): 292-298.
    [10] Liu H C, Sun Z Y, Peng Z H. Effects of salt stress on the growth and some physiological characteristics in Parthenocissus quinquefolia cuttings [J]. Sci Silv Sin, 2004, 40(6): 63-67. 刘会超, 孙振元, 彭镇华. NaCl胁迫对五叶地锦生长及某些生 理特性的影响 [J]. 林业科学, 2004, 40(6): 63-67.
    [11] Wang C, Zhu Y L, Yang L F, et al. Screening of vegetable soybean cultivars for salt tolerance and their physiological characteristics[J]. Jiangsu J Agri Sci, 2009, 25(3): 621-627. 王聪, 朱月林, 杨立飞, 等. 菜用大豆耐盐品种的筛选及其耐盐 生理特性 [J]. 江苏农业学报, 2009, 25(3): 621-627.
    [12] Guo X M, Li Q, Wang X, et al. Effects of salt stress on the physiological characteristics of sweet potato seedling [J]. Jiangsu Agri Sci, 2011, 39(3): 107-109. 过晓明, 李强, 王欣, 等. 盐胁迫对甘薯幼苗生理特性的影响[J]. 江苏农业科学, 2011, 39(3): 107-109.
    [13] Yan H X, Xu B L, Liang Q L, et al. Effects of chlorophyll content and stoma density on pumpkin resistance to powdery mildew [J]. Plant Protect, 2009, 35(1): 79-81. 颜惠霞, 徐秉良, 梁巧兰, 等. 南瓜品种对白粉病的抗病性与 叶绿素含量和气孔密度的相关性 [J]. 植物保护, 2009, 35(1): 79-81.
    [14] Ou Z Y. Relationship between chlorophyll content and disease resistance of plant [J]. Anhui Agri Sci Bull, 2007, 13(6): 135-135. 欧志远. 叶绿素含量与植物抗病性的关系 [J]. 安徽农学通报, 2007, 13(6): 135-135.
    [15] Xu B L, Li M Q, Yu J H, et al. The relation between the contents of chlorophyll in alfalfa varieties and the resistance to powder mildew of Alfalfa [J]. Pratacult Sci, 2005, 22(4): 72-74. 徐秉良, 李敏权, 郁继华, 等. 苜蓿对白粉病的抗性与叶绿素含 量的关系 [J]. 草业科学, 2005, 22(4): 72-74.
    [16] Morillon R, Chrispeels M J. The role of ABA and the transpiration stream in the regulation of the osmotic water permeability of leaf cells [J]. Proc Natl Acad Sci USA, 2001, 98(24): 14138-14143.
    [17] Zhang H. Role of abscisic acid in plant hardiness physiology [J]. J Anhui Agri Sci, 2013, 41(2): 490-491,527.张会. 脱落酸在植物抗性生理中的作用 [J]. 安徽农业科学, 2013, 41(2): 490-491,527.
    [18] Hu X L, Liu R X, Mao X J. Signal cascads of antioxidative defenses induced by water stress-accumulated ABA [J]. Acta Bot Boreal-Occid Sin, 2007, 27(5): 1061-1071. 胡秀丽, 刘瑞侠, 毛训甲. 水分胁迫积累的ABA诱导抗氧化 防护系统的信号级联 [J]. 西北植物学报, 2007, 27(5): 1061-1071.
    [19] Loik M E, Nobel P S. Exogenous abscisic acid mimics cold acclimation for cacti differing in freezing tolerance [J]. Plant Physiol, 1993, 103(3): 871-876.
    [20] Du S H, Bu Z G, Liu Y. Study on the change of endogenous hormones of jujube caused by phytoplasma infection [J]. North Hort, 2013(13): 12-15. 杜绍华, 卜志国, 刘洋. 植原体浸染对枣树内源激素含量的影 响 [J]. 北方园艺, 2013(13): 12-15.
    [21] Yang J H, Chen T, Wang X L. Effect of enhanced UV-B radiation on endogenous ABA and free proline contents in wheat leaves [J]. Acta Ecol Sin, 2000, 20(1): 39-42. 杨景宏, 陈拓, 王勋陵. 增强UV-B辐射对小麦叶片内源ABA和 游离脯氨酸的影响 [J]. 生态学报, 2000, 20(1): 39-42.
    [22] Wang X, Yu J, Yang Y, et al. Changes of endogenous hormones of winter wheat varieties with different cold-resistances under low temperature [J]. J Tritic Crops, 2009, 29(5): 827-831. 王兴, 于晶, 杨阳, 等. 低温条件下不同抗寒性冬小麦内源激素 的变化 [J]. 麦类作物学报, 2009, 29(5): 827-831.
    [23] Lafuente M T, Belver A, Guye M G, et al. Effect of temperature conditioning on chilling injury of cucumber cotyledons [J]. Plant Physiol, 1991, 95(2): 443-449.
    [24] Yu L, Guo S R, Zhu W M, et al. Effects of tomato yellow leaf curl virus on photosynthetic characteristics and chloroplast ultrastructure of the tomato leaves [J]. Acta Bot Boreal-Occid Sin, 2011, 31(7): 1355-1359. 于力, 郭世荣, 朱为民, 等. 番茄黄化曲叶病毒对番茄叶片光 合特性和叶绿体超微结构的影响 [J]. 西北植物学报, 2011, 31(7): 1355-1359.
    [25] Farguhar G D, Sharkea T D. Stomatal conductance and photosynthesis[J]. Ann Rev Plant Physiol, 1982, 33(1): 317-345.
    [26] Guo S Z, Zhang M H, Deng C Y, et al. Effect of drought stress on photosynthetic characteristics and ultrastructure of mesophyll cells in three cultivars of Jaminum sambac [J]. J Trop Subtrop Bot, 2013, 21(2): 123-129. 郭素枝, 张明辉, 邓传远, 等. 干旱胁迫对茉莉3个品种叶片光 合特性和超微结构的影响 [J]. 热带亚热带植物学报, 2013, 21(2): 123-129.
    [27] Vassileva V, Simova-Stoilova L, Demirevska K. Variety-specific respones of wheat (Triticum aestivum L.) leaf mitochondria to drought stress [J]. J Plant Res, 2009, 122(4): 445-454.
    [28] Ke Y Q, Pan T G. Effects of salt stress on the ultrastructure of chloroplast and the activities of some protective enzymes in leaves of sweet potato [J]. Acta Phytophysiol Sin, 1999, 25(3): 229-233. 柯玉琴, 潘廷国. NaCl胁迫对甘薯叶片叶绿体超微结构及一 些酶活性的影响 [J]. 植物生理学报, 1999, 25(3): 229-233.
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引用本文

王伟英,邱永祥,邱珊莲,柯玉琴,潘廷国.薯瘟菌对甘薯叶片光合特性及叶绿体结构的影响[J].热带亚热带植物学报,2014,22(6):610~616

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  • 收稿日期:2014-04-23
  • 最后修改日期:2014-06-16
  • 录用日期:2014-08-18
  • 在线发布日期: 2014-11-25
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